Paper · 1983 · Journal of Experimental Botany

Nucleotide Levels and Loss of Vigour and Viability in Germinating Wheat Embryos

SHEILA A. STANDARD; DAVID PERRET; CLIFFORD M. BRAY

7
claims
13
verbatim quotes
28
citations
1
research areas
—
Not open access

Abstract

At an optimal germination temperature loss of viability in wheat seed is reflected in reduced levels of nucleoside triphosphates and nucleotide sugars in the embryo compared to the levels found in high viability embryos during the early hours of imbibition. These differences are magnified on germination at 10° C and are accompanied by a greatly reduced rate of protein synthesis in embryos of low viability compared with the corresponding rate in embryos of high viability. Loss of vigour precedes loss of viability in seed but differences in biochemical parameters between high and medium vigour seed do not become apparent unless germination under stress conditions, e. g. low temperature, occurs. Under these stress conditions, wheat seed of reduced vigour can be distinguished from high vigour seed lots of similar viability by the decreased levels of nucleotides and nucleotide sugars found in embryos from reduced vigour seed lots during the early hours of imbibition and by reduced rates of protein synthesis in these embryos during this same period. Nucleotide analysis is a potentially useful method for rapid assessment of the viability or vigour of a seed lot.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

At optimal germination temperature, low-viability wheat embryos had reduced levels of nucleoside triphosphates and nucleotide sugars compared with high-viability embryos in early imbibition.

Organ / stage
embryoe0
Outcome
levels of nucleoside triphosphates and nucleotide sugarse0
Direction
reducede0
Qualifier
during the early hours of imbibitione0
Comparator
high viability embryose0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At an optimal germination temperature loss of viability in wheat seed is reflected in reduced levels of nucleoside triphosphates and nucleotide sugars in the embryo compared to the levels found in high viability embryos during the early hours of imbibition”

✓ located verbatim in the source · abstract, characters 0–256 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Germination at 10° C magnified the reduction in the rate of protein synthesis in low-viability wheat embryos compared with high-viability embryos.

Organ / stage
embryose1
Treatment
germination at 10° Ce1
Outcome
rate of protein synthesise1
Direction
greatly reducede1
Comparator
embryos of high viabilitye1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At an optimal germination temperature loss of viability in wheat seed is reflected in reduced levels of nucleoside triphosphates and nucleotide sugars in the embryo compared to the levels found in high viability embryos during the early hours of imbibition”

✓ located verbatim in the source · abstract, characters 0–256 · show in text ↑
e1

“These differences are magnified on germination at 10° C and are accompanied by a greatly reduced rate of protein synthesis in embryos of low viability compared with the corresponding rate in embryos of high viability”

✓ located verbatim in the source · abstract, characters 258–474 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Under stress conditions, reduced-vigour wheat seed lots had decreased levels of nucleotides and nucleotide sugars in embryos compared with high-vigour lots of similar viability.

Organ / stage
embryose1
Treatment
stress conditionse1
Outcome
levels of nucleotides and nucleotide sugarse1
Direction
decreasede1
Comparator
high vigour seed lots of similar viabilitye1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At an optimal germination temperature loss of viability in wheat seed is reflected in reduced levels of nucleoside triphosphates and nucleotide sugars in the embryo compared to the levels found in high viability embryos during the early hours of imbibition”

✓ located verbatim in the source · abstract, characters 0–256 · show in text ↑
e1

“Under these stress conditions, wheat seed of reduced vigour can be distinguished from high vigour seed lots of similar viability by the decreased levels of nucleotides and nucleotide sugars found in embryos from reduced vigour seed lots during the early hours of imbibition and by reduced rates of protein synthesis in these embryos during this same period”

✓ located verbatim in the source · abstract, characters 702–1058 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Under stress conditions, reduced-vigour wheat embryos had reduced rates of protein synthesis compared with high-vigour lots of similar viability.

Organ / stage
embryose1
Treatment
stress conditionse1
Outcome
rates of protein synthesise1
Direction
reducede1
Comparator
high vigour seed lots of similar viabilitye1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At an optimal germination temperature loss of viability in wheat seed is reflected in reduced levels of nucleoside triphosphates and nucleotide sugars in the embryo compared to the levels found in high viability embryos during the early hours of imbibition”

✓ located verbatim in the source · abstract, characters 0–256 · show in text ↑
e1

“Under these stress conditions, wheat seed of reduced vigour can be distinguished from high vigour seed lots of similar viability by the decreased levels of nucleotides and nucleotide sugars found in embryos from reduced vigour seed lots during the early hours of imbibition and by reduced rates of protein synthesis in these embryos during this same period”

✓ located verbatim in the source · abstract, characters 702–1058 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Loss of vigour precedes loss of viability in wheat seed.

Outcome
Loss of vigoure1
Direction
precedese1
Qualifier
loss of viabilitye1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At an optimal germination temperature loss of viability in wheat seed is reflected in reduced levels of nucleoside triphosphates and nucleotide sugars in the embryo compared to the levels found in high viability embryos during the early hours of imbibition”

✓ located verbatim in the source · abstract, characters 0–256 · show in text ↑
e1

“Loss of vigour precedes loss of viability in seed but differences in biochemical parameters between high and medium vigour seed do not become apparent unless germination under stress conditions, e. g. low temperature, occurs”

✓ located verbatim in the source · abstract, characters 476–700 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Biochemical differences between high- and medium-vigour wheat seed did not appear unless germination occurred under stress conditions.

Treatment
germination under stress conditionse1
Outcome
differences in biochemical parameterse1
Direction
do not become apparente1
Comparator
high and medium vigour seede1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At an optimal germination temperature loss of viability in wheat seed is reflected in reduced levels of nucleoside triphosphates and nucleotide sugars in the embryo compared to the levels found in high viability embryos during the early hours of imbibition”

✓ located verbatim in the source · abstract, characters 0–256 · show in text ↑
e1

“Loss of vigour precedes loss of viability in seed but differences in biochemical parameters between high and medium vigour seed do not become apparent unless germination under stress conditions, e. g. low temperature, occurs”

✓ located verbatim in the source · abstract, characters 476–700 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Nucleotide analysis is a potentially useful method for rapid assessment of the viability or vigour of wheat seed lots.

Outcome
rapid assessment of the viability or vigour of a seed lote1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At an optimal germination temperature loss of viability in wheat seed is reflected in reduced levels of nucleoside triphosphates and nucleotide sugars in the embryo compared to the levels found in high viability embryos during the early hours of imbibition”

✓ located verbatim in the source · abstract, characters 0–256 · show in text ↑
e1

“Nucleotide analysis is a potentially useful method for rapid assessment of the viability or vigour of a seed lot”

✓ located verbatim in the source · abstract, characters 1060–1172 · show in text ↑

processing · quality: protein · Triticum aestivum (wheat)Positive and negative results

25 papers: 13 positive, 12 negative or no effect

11 papers in the same direction as this paper · 13 in the opposite direction

  1. positive
  2. positive
  3. positive

21 papers more in this group

See all 25 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.