Abstract
Fructans are the major storage carbohydrate in vegetative tissues of wheat (Triticum aestivum L.). Fructan:fructan fructosyl transferase (FFT) catalyzes fructosyl transfer between fructan molecules to elongate the fructan chain. The objective of this research was to isolate this activity in wheat. Wheat (cv Caldwell) plants grown at 25 degrees C for 3 weeks were transferred to 10 degrees C to induce fructan synthesis. From the leaf blades kept at 10 degrees C for 4 days, fructosyl transferase activity was purified using salt precipitation and a series of chromatographic procedures including size exclusion, anion-exchange, and affinity chromatography. The transferase activity was free from invertase and other fructan-metabolizing activities. Fructosyl transferase had a broad pH spectrum with a peak activity at 6.5. The temperature optimum was 30 degrees C. The activity was specific for fructosyl transfer from beta(2-->1)-linked 1-kestose or fructan to sucrose and beta(2-->1) fructosyl transfer to other fructans (1-FFT). Fructosyl transfer from oligofructans to sucrose was most efficient when 1-kestose was used as donor molecule and declined as the degree of polymerization of the donor increased from 3 to 5. 1-FFT catalyzed the in vitro synthesis of inulin tetra- and penta-saccharides from 1-kestose; however, formation of the tetrasaccharide was greatly reduced at high sucrose concentration. 6-Kestose could not act as donor molecule, but could accept a fructosyl moiety from 1-kestose to produce bifurcose and a tetrasaccharide having a beta(2-->1) fructose attached to the terminal fructose of 6-kestose. The role of this FFT activity in the synthesis of fructan in wheat is discussed.
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Claims 10
Claim · model-written summary
Purified fructosyl transferase activity was free of invertase and other fructan-metabolizing activities.
- Taxon
- Wheat
Triticum aestivumt0 - Gene
- transferasee0
- Outcome
- free from invertase and other fructan-metabolizing activitiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transferase activity was free from invertase and other fructan-metabolizing activities”
t0“Wheat”
Claim · model-written summary
Fructosyl transferase activity peaked at pH 6.5.
- Taxon
- WheatTriticum aestivumt0
- Gene
- Fructosyl transferasee0
- Outcome
- peak activitye0
- Value
- 6.5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fructosyl transferase had a broad pH spectrum with a peak activity at 6.5”
t0“Wheat”
Claim · model-written summary
The temperature optimum was 30 degrees C.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- temperature optimume0
- Value
- 30 degrees Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The temperature optimum was 30 degrees C”
t0“Wheat”
Claim · model-written summary
The enzyme specifically transferred fructosyl groups from beta(2-->1)-linked donors to sucrose and other fructans.
- Taxon
- WheatTriticum aestivumt0
- Gene
- 1-FFTe0
- Outcome
- fructosyl transfer from beta(2-->1)-linked 1-kestose or fructan to sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity was specific for fructosyl transfer from beta(2-->1)-linked 1-kestose or fructan to sucrose and beta(2-->1) fructosyl transfer to other fructans (1-FFT)”
t0“Wheat”
Claim · model-written summary
Fructosyl transfer from oligofructans to sucrose was most efficient with 1-kestose donor.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- 1-kestosee0
- Outcome
- Fructosyl transfer from oligofructans to sucrosee0
- Direction
- most efficiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fructosyl transfer from oligofructans to sucrose was most efficient when 1-kestose was used as donor molecule”
t0“Wheat”
Claim · model-written summary
Transfer efficiency declined as donor polymerization increased from 3 to 5.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- degree of polymerization of the donore0
- Outcome
- Fructosyl transfere0
- Direction
- declinede0
- Value
- 3 to 5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fructosyl transfer from oligofructans to sucrose was most efficient when 1-kestose was used as donor molecule and declined as the degree of polymerization of the donor increased from 3 to 5”
t0“Wheat”
Claim · model-written summary
1-FFT synthesized inulin tetra- and penta-saccharides from 1-kestose in vitro.
- Taxon
- WheatTriticum aestivumt0
- Gene
- 1-FFTe0
- Outcome
- synthesis of inulin tetra- and penta-saccharidese0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1-FFT catalyzed the in vitro synthesis of inulin tetra- and penta-saccharides from 1-kestose”
t0“Wheat”
Claim · model-written summary
High sucrose concentration greatly reduced tetrasaccharide formation.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- high sucrose concentratione0
- Outcome
- formation of the tetrasaccharidee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“formation of the tetrasaccharide was greatly reduced at high sucrose concentration”
t0“Wheat”
Claim · model-written summary
6-Kestose could not serve as a donor molecule.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- could not act as donor moleculee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6-Kestose could not act as donor molecule”
t0“Wheat”
Claim · model-written summary
6-Kestose accepted a fructosyl moiety from 1-kestose to produce bifurcose and a tetrasaccharide.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- produce bifurcose and a tetrasaccharidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“could accept a fructosyl moiety from 1-kestose to produce bifurcose and a tetrasaccharide having a beta(2-->1) fructose attached to the terminal fructose of 6-kestose”
t0“Wheat”
The same questions in other papers
sucrose · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
2 papers more in this group
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