Abstract
Somatic embryogenesis (SE) is the process by which cells become dedifferentiated and reprogram to follow an embryogenic pathway. It is important for regeneration of transgenic plants as well as for propagation of certain genotypes. However, competence for SE varies, even among genotypes of a species, and the basis for this variation is not understood. We have found that the MADS-box transcription factor (Glycine max) AGAMOUS-Like 15 [(Gm)AGL15] promotes SE in Arabidopsis and in soybean when overexpressed. In soybean, part of the promotion of SE is via GmAGL15-mediated control of ethylene biosynthesis and response. Addition of ACC, the precursor to ethylene, to culture media enhanced SE in Arabidopsis and soybean. Transcription factors important for embryogenesis responded directly to GmAGL15 and to ethylene accumulation. Here we correlate ethylene production and patterns of gene expression with SE potential of soybean genotypes. However, other results indicate that there is not a complete positive correlation between ethylene production and SE, indicating that the interactions between hormones, gene expression and developmental outcomes are complex.
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Claims 9
Claim · model-written summary
GmAGL15 overexpression promotes somatic embryogenesis in Arabidopsis.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“[(Gm)AGL15] promotes SE in Arabidopsis and in soybean when overexpressed”
Claim · model-written summary
GmAGL15 overexpression promotes somatic embryogenesis in soybean.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“[(Gm)AGL15] promotes SE in Arabidopsis and in soybean when overexpressed”
Claim · model-written summary
GmAGL15 promotes soybean somatic embryogenesis partly through ethylene biosynthesis and response.
- Taxon
- soybean
Glycine maxe0 - Gene
- GmAGL15e0
- Compound
- ethylenee0
- Outcome
- promotion of SEe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In soybean, part of the promotion of SE is via GmAGL15-mediated control of ethylene biosynthesis and response”
Claim · model-written summary
ACC addition enhanced somatic embryogenesis in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Addition of ACCe0
- Outcome
- SEe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of ACC, the precursor to ethylene, to culture media enhanced SE in Arabidopsis and soybean”
Claim · model-written summary
ACC addition enhanced somatic embryogenesis in soybean.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Addition of ACCe0
- Outcome
- SEe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of ACC, the precursor to ethylene, to culture media enhanced SE in Arabidopsis and soybean”
Claim · model-written summary
Embryogenesis transcription factors responded directly to GmAGL15.
- Taxon
- Glycine maxGlycine maxt0
- Gene
- GmAGL15e0
- Outcome
- Transcription factors important for embryogenesis responded directlye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcription factors important for embryogenesis responded directly to GmAGL15 and to ethylene accumulation”
t0“Glycine max”
Claim · model-written summary
Embryogenesis transcription factors responded directly to ethylene accumulation.
- Taxon
- Glycine maxGlycine maxt0
- Compound
- ethylenee0
- Outcome
- Transcription factors important for embryogenesis responded directlye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcription factors important for embryogenesis responded directly to GmAGL15 and to ethylene accumulation”
t0“Glycine max”
Claim · model-written summary
Ethylene production and gene-expression patterns correlate with somatic embryogenesis potential among soybean genotypes.
- Taxon
- soybeanGlycine maxe0
- Material
- soybean genotypese0
- Compound
- ethylenee0
- Outcome
- SE potentiale0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we correlate ethylene production and patterns of gene expression with SE potential of soybean genotypes”
Claim · model-written summary
Ethylene production is not completely positively correlated with somatic embryogenesis.
- Taxon
- Glycine maxGlycine maxt0
- Compound
- ethylenee0
- Outcome
- SEe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there is not a complete positive correlation between ethylene production and SE”
t0“Glycine max”
The same questions in other papers
1-aminocyclopropanecarboxylic acid · growth · Glycine max (soybean)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1-aminocyclopropanecarboxylic acid · growth · ArabidopsisPositive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.