Abstract
The root of Arabidopsis thaliana is used as a model system to unravel the molecular nature of cell elongation and its arrest. From a micro-array performed on roots that were treated with aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene, a Small auxin-up RNA (SAUR)-like gene was found to be up regulated. As it appeared as the 76th gene in the family, it was named SAUR76. Root and leaf growth of overexpression lines ectopically expressing SAUR76 indicated the possible involvement of the gene in the division process. Using promoter::GUS and GFP lines strong expression was seen in endodermal and pericycle cells at the end of the elongation zone and during several stages of lateral root primordia development. ACC and IAA/NAA were able to induce a strong up regulation of the gene and changed the expression towards cortical and even epidermal cells at the beginning of the elongation zone. Confirmation of this up regulation of expression was delivered using qPCR, which also indicated that the expression quickly returned to normal levels when the inducing IAA-stimulus was removed, a behaviour also seen in other SAUR genes. Furthermore, confocal analysis of protein-GFP fusions localized the protein in the nucleus, cytoplasm and plasma membrane. SAUR76 expression was quantified in several mutants in ethylene and auxin-related pathways, which led to the conclusion that the expression of SAUR76 is mainly regulated by the increase in auxin that results from the addition of ACC, rather than by ACC itself.
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Claims 8
Claim · model-written summary
ACC treatment upregulated the SAUR-like gene later named SAUR76 in roots.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Treatment
- aminocyclopropane-1-carboxylic acid (ACC)e0
- Gene
- Small auxin-up RNA (SAUR)-like genee0
- Outcome
- up regulatede0
- Direction
- up regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“roots that were treated with aminocyclopropane-1-carboxylic acid (ACC), the precursor of ethylene, a Small auxin-up RNA (SAUR)-like gene was found to be up regulated”
e1“The root of Arabidopsis thaliana”
Claim · model-written summary
Overexpression line growth suggested SAUR76 involvement in cell division.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- overexpression linese0
- Organ / stage
- Root and leafe0
- Gene
- SAUR76e0
- Outcome
- possible involvement of the gene in the division processe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root and leaf growth of overexpression lines ectopically expressing SAUR76 indicated the possible involvement of the gene in the division process”
e1“Arabidopsis thaliana”
Claim · model-written summary
SAUR76 was strongly expressed in endodermal and pericycle cells and developing lateral root primordia.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Organ / stage
- endodermal and pericycle cellse0
- Gene
- SAUR76e2
- Outcome
- strong expressione0
- Qualifier
- during several stages of lateral root primordia developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strong expression was seen in endodermal and pericycle cells at the end of the elongation zone and during several stages of lateral root primordia development”
e1“Arabidopsis thaliana”
e2“it was named SAUR76”
Claim · model-written summary
ACC and IAA/NAA induced strong SAUR76 upregulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- ACC and IAA/NAAe0
- Gene
- SAUR76e2
- Outcome
- up regulation of the genee0
- Direction
- up regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC and IAA/NAA were able to induce a strong up regulation of the gene”
e1“Arabidopsis thaliana”
e2“it was named SAUR76”
Claim · model-written summary
ACC and IAA/NAA shifted SAUR76 expression toward cortical and epidermal cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- ACC and IAA/NAAe0
- Gene
- SAUR76e2
- Outcome
- expression towards cortical and even epidermal cellse0
- Direction
- changede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ACC and IAA/NAA were able to induce a strong up regulation of the gene and changed the expression towards cortical and even epidermal cells at the beginning of the elongation zone”
e1“Arabidopsis thaliana”
e2“it was named SAUR76”
Claim · model-written summary
SAUR76 expression quickly returned to normal after IAA removal.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- IAA-stimulus was removede0
- Gene
- SAUR76e2
- Outcome
- expressione0
- Direction
- returned to normal levelse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression quickly returned to normal levels when the inducing IAA-stimulus was removed”
e1“Arabidopsis thaliana”
e2“it was named SAUR76”
Claim · model-written summary
SAUR76 protein localized to the nucleus, cytoplasm and plasma membrane.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Gene
- SAUR76e3
- Outcome
- localized the protein in the nucleus, cytoplasm and plasma membranee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the protein in the nucleus, cytoplasm and plasma membrane”
e1“confocal analysis of protein-GFP fusions localized the protein in the nucleus, cytoplasm and plasma membrane”
e2“Arabidopsis thaliana”
e3“it was named SAUR76”
Claim · model-written summary
SAUR76 expression appears mainly regulated by ACC-induced auxin increase rather than ACC itself.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- SAUR76e0
- Compound
- auxine0
- Outcome
- expression of SAUR76e0
- Qualifier
- rather than by ACC itselfe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of SAUR76 is mainly regulated by the increase in auxin that results from the addition of ACC, rather than by ACC itself”
e1“Arabidopsis thaliana”
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