Abstract
The effect of salt stress on polypeptide and mRNA levels in roots of two barley (Hordeum vulgare L.) cultivars differing in salt tolerance (cv CM 72, tolerant; cv Prato, sensitive) was analyzed using two-dimensional polyacrylamide gel electrophoresis. Preliminary experiments indicated that germination of Prato was inhibited significantly in the presence of NaCl, but growth of the surviving Prato seedlings was not substantially different from that of CM 72. Fluorographs of two-dimensional gels containing in vivo labeled polypeptides or in vitro translation products were computer analyzed to identify and quantitate changes that resulted when plants were grown in the presence of 200 millimolar NaCl for 6 days. The patterns of in vivo labeled polypeptides and in vitro products of CM 72 and Prato were qualitatively the same. Salt caused quantitative changes in numerous polypeptides and translatable mRNAs, but, overall, the changes were relatively small. Salt did not induce the synthesis of unique polypeptides or translatable mRNAs and did not cause any to disappear. Because of the similarities of the two cultivars with respect to growth and polypeptide patterns and the slight changes in polypeptide and translation product levels caused by salt, specific polypeptides or translatable mRNAs that are related to salt tolerance in barley could not be identified.
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Claims 8
Claim · model-written summary
NaCl significantly inhibited Prato germination.
- Taxon
- barley (Hordeum vulgare L.)
.jpg?width=60)
Hordeum vulgaree1 - Material
- Pratoe0
- Treatment
- NaCle0
- Outcome
- germinatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“germination of Prato was inhibited significantly in the presence of NaCl”
e1“barley (Hordeum vulgare L.)”
Claim · model-written summary
Surviving Prato seedlings grew similarly to CM 72.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Material
- Pratoe0
- Outcome
- growthe0
- Direction
- not substantially differente0
- Comparator
- CM 72e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“growth of the surviving Prato seedlings was not substantially different from that of CM 72”
e1“barley (Hordeum vulgare L.)”
Claim · model-written summary
The cultivars had qualitatively the same in vivo polypeptide patterns.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Material
- CM 72 and Pratoe0
- Outcome
- patterns of in vivo labeled polypeptidese0
- Direction
- qualitatively the samee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The patterns of in vivo labeled polypeptides and in vitro products of CM 72 and Prato were qualitatively the same”
e1“barley (Hordeum vulgare L.)”
Claim · model-written summary
The cultivars had qualitatively the same in vitro translation products.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Material
- CM 72 and Pratoe0
- Outcome
- in vitro productse0
- Direction
- qualitatively the samee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The patterns of in vivo labeled polypeptides and in vitro products of CM 72 and Prato were qualitatively the same”
e1“barley (Hordeum vulgare L.)”
Claim · model-written summary
Salt caused relatively small quantitative changes in many polypeptides and translatable mRNAs.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- Salte0
- Outcome
- numerous polypeptides and translatable mRNAse0
- Direction
- changese0
- Qualifier
- relatively smalle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salt caused quantitative changes in numerous polypeptides and translatable mRNAs, but, overall, the changes were relatively small”
e1“barley (Hordeum vulgare L.)”
Claim · model-written summary
Salt did not induce unique polypeptides or translatable mRNAs.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- Salte0
- Outcome
- synthesis of unique polypeptides or translatable mRNAse0
- Direction
- did not inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salt did not induce the synthesis of unique polypeptides or translatable mRNAs”
e1“barley (Hordeum vulgare L.)”
Claim · model-written summary
Salt did not eliminate any polypeptides or translatable mRNAs.
- Taxon
- barley (Hordeum vulgare L.)Hordeum vulgaree1
- Treatment
- Salte0
- Outcome
- any to disappeare0
- Direction
- did not causee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salt did not induce the synthesis of unique polypeptides or translatable mRNAs and did not cause any to disappear”
e1“barley (Hordeum vulgare L.)”
Claim · model-written summary
The study could not identify specific proteins or mRNAs linked to barley salt tolerance.
- Taxon
- barleyHordeum vulgaree0
- Outcome
- specific polypeptides or translatable mRNAse0
- Direction
- could not be identifiede0
- Qualifier
- salt tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“specific polypeptides or translatable mRNAs that are related to salt tolerance in barley could not be identified”
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See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.