Paper · 2010 · Journal of Agricultural and Food Chemistry

Relationship between Fresh-Packaged Spinach Leaves Exposed to Continuous Light or Dark and Bioactive Contents: Effects of Cultivar, Leaf Size, and Storage Duration

Gene E. Lester; Donald J. Makus; D. Mark Hodges

5
claims
13
verbatim quotes
90
citations
1
research areas
—
Not open access

Abstract

Current retail marketing conditions allow produce to receive artificial light 24 h per day during its displayed shelf life. Essential human-health vitamins [ascorbic acid (vit C), folate (vit B(9)), phylloquinone (vit K(1)), alpha-tocopherol (vit E), and the carotenoids lutein, violaxanthin, zeaxanthin, and beta-carotene (provit A)] also are essential for photosynthesis and are biosynthesized in plants by light conditions even under chilling temperatures. Spinach leaves, notably abundant in the aforementioned human-health compounds, were harvested from flat-leaf 'Lazio' and crinkle-leafed 'Samish' cultivars at peak whole-plant maturity as baby (top- and midcanopy) and larger (lower-canopy) leaves. Leaves were placed as a single layer in commercial, clear-polymer retail boxes and stored at 4 degrees C for up to 9 days under continuous light (26.9 micromol.m(2 ).s) or dark. Top-canopy, baby-leaf spinach generally had higher concentrations of all bioactive compounds, on a dry weight basis, with the exception of carotenoids, than bottom-canopy leaves. All leaves stored under continuous light generally had higher levels of all bioactive compounds, except beta-carotene and violaxanthin, and were more prone to wilting, especially the flat-leafed cultivar. All leaves stored under continuous darkness had declining or unchanged levels of the aforementioned bioactive compounds. Findings from this study revealed that spinach leaves exposed to simulated retail continuous light at 4 degrees C, in clear plastic containers, were overall more nutritionally dense (enriched) than leaves exposed to continuous darkness.

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Claims 5

Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Top-canopy, baby-leaf spinach generally had higher concentrations of bioactive compounds than bottom-canopy leaves, except carotenoids.

Organ / stage
Top-canopy, baby-leafe1
Outcome
bioactive compoundse1
Direction
highere1
Qualifier
with the exception of carotenoidse1
Comparator
bottom-canopy leavese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Spinach Leaves”

✓ located verbatim in the source · title, characters 36–50 · show in text ↑
e1

“Top-canopy, baby-leaf spinach generally had higher concentrations of all bioactive compounds, on a dry weight basis, with the exception of carotenoids, than bottom-canopy leaves”

✓ located verbatim in the source · abstract, characters 885–1062 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Leaves stored under continuous light generally had higher levels of bioactive compounds than leaves stored under continuous darkness, except beta-carotene and violaxanthin.

Treatment
continuous lighte1
Outcome
bioactive compoundse1
Direction
highere1
Qualifier
except beta-carotene and violaxanthine1
Comparator
continuous darknesse2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Spinach Leaves”

✓ located verbatim in the source · title, characters 36–50 · show in text ↑
e1

“All leaves stored under continuous light generally had higher levels of all bioactive compounds, except beta-carotene and violaxanthin”

✓ located verbatim in the source · abstract, characters 1064–1198 · show in text ↑
e2

“All leaves stored under continuous darkness had declining or unchanged levels of the aforementioned bioactive compounds”

✓ located verbatim in the source · abstract, characters 1269–1388 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Leaves stored under continuous light were more prone to wilting than leaves stored under continuous darkness, especially the flat-leafed cultivar.

Material
flat-leafed cultivare2
Treatment
continuous lighte1
Outcome
wiltinge2
Direction
more pronee2
Comparator
continuous darknesse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Spinach Leaves”

✓ located verbatim in the source · title, characters 36–50 · show in text ↑
e1

“All leaves stored under continuous light generally had higher levels of all bioactive compounds, except beta-carotene and violaxanthin”

✓ located verbatim in the source · abstract, characters 1064–1198 · show in text ↑
e2

“were more prone to wilting, especially the flat-leafed cultivar”

✓ located verbatim in the source · abstract, characters 1204–1267 · show in text ↑
e3

“All leaves stored under continuous darkness had declining or unchanged levels of the aforementioned bioactive compounds”

✓ located verbatim in the source · abstract, characters 1269–1388 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Leaves stored under continuous darkness had declining or unchanged levels of bioactive compounds compared with continuous light.

Treatment
continuous darknesse1
Outcome
bioactive compoundse1
Direction
declining or unchangede1
Comparator
continuous lighte2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Spinach Leaves”

✓ located verbatim in the source · title, characters 36–50 · show in text ↑
e1

“All leaves stored under continuous darkness had declining or unchanged levels of the aforementioned bioactive compounds”

✓ located verbatim in the source · abstract, characters 1269–1388 · show in text ↑
e2

“All leaves stored under continuous light generally had higher levels of all bioactive compounds”

✓ located verbatim in the source · abstract, characters 1064–1159 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Spinach leaves exposed to simulated retail continuous light at 4 degrees C in clear plastic containers were overall more nutritionally dense than leaves in continuous darkness.

Treatment
simulated retail continuous lighte0
Outcome
nutritionally dense (enriched)e0
Direction
moree0
Comparator
continuous darknesse0
Setting
clear plastic containerse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“spinach leaves exposed to simulated retail continuous light at 4 degrees C, in clear plastic containers, were overall more nutritionally dense (enriched) than leaves exposed to continuous darkness”

✓ located verbatim in the source · abstract, characters 1429–1625 · show in text ↑

light · disease · Spinacia oleracea (spinach)Positive and negative results

4 papers: 1 positive, 3 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative
See all 4 papers →

light · quality: phytochemicals · Spinacia oleracea (spinach)Positive and negative results

9 papers: 8 positive, 1 negative or no effect

7 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.