Abstract
We have previously reported the expression of four different maize D cyclins during seed germination and showed that cytokinins and auxins stimulate the expression of every cyclin in a differential way. In this paper we characterize the behavior at the protein level of two of these cyclins, CycD5 and CycD4;1. Antibodies were raised against CycD5;2 (which very likely also recognizes D5;1) and CycD4;1 and Western blot studies demonstrated that neither BA nor indol-3 acetic acid (IAA) stimulate cyclin accumulation during germination, compared with control levels. However, phytohormones, particularly IAA, modify the kinase activity associated to D cyclins preferentially at early hours of germination. The associated kinase moiety to D cyclins appears to be of a Cdk-A type because this protein immunoprecipitates with D cyclins and because kinase activity is strongly inhibited by both olomoucine and also by a peptide corresponding to the carboxy end of a maize kip related protein (KRP) protein. There is thus no correlation between mRNA and protein expression for these maize D cyclins during seed germination, although phytohormones may stimulate a signaling cascade that stimulates activation of protein kinase activity in cyclin-Cdk complexes.
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Claims 7
Claim · model-written summary
BA did not stimulate D cyclin accumulation during germination versus controls.
- Organ / stage
- germinatione0
- Treatment
- BAe0
- Gene
- cycline0
- Outcome
- cyclin accumulatione0
- Direction
- neithere0
- Comparator
- control levelse0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“neither BA nor indol-3 acetic acid (IAA) stimulate cyclin accumulation during germination, compared with control levels”
t0“maize”
Claim · model-written summary
IAA did not stimulate D cyclin accumulation during germination versus controls.
- Organ / stage
- germinatione0
- Treatment
- indol-3 acetic acid (IAA)e0
- Gene
- cycline0
- Outcome
- cyclin accumulatione0
- Direction
- neithere0
- Comparator
- control levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“neither BA nor indol-3 acetic acid (IAA) stimulate cyclin accumulation during germination, compared with control levels”
t0“maize”
Claim · model-written summary
IAA modified D cyclin associated kinase activity, particularly early in germination.
- Treatment
- IAAe0
- Gene
- D cyclinse0
- Outcome
- kinase activity associated to D cyclinse0
- Qualifier
- early hours of germinatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phytohormones, particularly IAA, modify the kinase activity associated to D cyclins preferentially at early hours of germination”
t0“maize”
Claim · model-written summary
A Cdk-A type protein immunoprecipitated with D cyclins.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this protein immunoprecipitates with D cyclins”
t0“maize”
Claim · model-written summary
Olomoucine strongly inhibited D cyclin associated kinase activity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“kinase activity is strongly inhibited by both olomoucine and also by a peptide corresponding to the carboxy end of a maize kip related protein (KRP) protein”
Claim · model-written summary
A maize KRP peptide strongly inhibited D cyclin associated kinase activity.
- Treatment
- a peptide corresponding to the carboxy end of a maize kip related protein (KRP) proteine0
- Gene
- maize kip related protein (KRP) proteine0
- Outcome
- kinase activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“kinase activity is strongly inhibited by both olomoucine and also by a peptide corresponding to the carboxy end of a maize kip related protein (KRP) protein”
Claim · model-written summary
D cyclin mRNA and protein expression did not correlate during germination.
- Organ / stage
- seed germinatione0
- Gene
- maize D cyclinse0
- Outcome
- protein expressione0
- Direction
- no correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no correlation between mRNA and protein expression for these maize D cyclins during seed germination”
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