Abstract
Translational regulation was evaluated for over 2000 genes by measurement of the proportion of individual mRNA species in polysomal (PS) complexes in leaves of non-stressed and moderately dehydration-stressed Arabidopsis. The amount of each mRNA in polysomes ranged from 23 to 97% in non-stressed leaves and was significantly reduced for a large portion of the genes (71%) in response to dehydration. The effect of dehydration on translational status varied extensively between mRNA species. Sixty per cent of the dehydration-inducible mRNAs with twofold or greater increase in abundance maintained PS levels in response to water-deficit stress, while 40% showed impaired ribosome loading (RL). PS association declined significantly for 92% of the mRNAs that displayed a strong decrease in abundance, indicating a relationship between translation and decreased gene transcription and/or mRNA stability. Interestingly, many mRNAs that encode proteins of similar biological function displayed coordinate translational regulation. Thus, the abundance of PS mRNA may provide a more accurate estimate of gene expression than total cellular mRNA because of extensive differential translational regulation.
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Claims 8
Claim · model-written summary
Polysomal mRNA proportions ranged from 23 to 97% in non-stressed leaves.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Organ / stage
- leavese0
- Outcome
- amount of each mRNA in polysomese0
- Value
- 23 to 97%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amount of each mRNA in polysomes ranged from 23 to 97% in non-stressed leaves”
t0“Arabidopsis thaliana”
Claim · model-written summary
Dehydration significantly reduced polysomal mRNA levels for 71% of genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- dehydratione0
- Outcome
- amount of each mRNA in polysomese1
- Direction
- reducede0
- Value
- 71%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significantly reduced for a large portion of the genes (71%) in response to dehydration”
e1“The amount of each mRNA in polysomes”
t0“Arabidopsis thaliana”
Claim · model-written summary
The translation response to dehydration varied among mRNA species.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- translational statuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of dehydration on translational status varied extensively between mRNA species.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Sixty percent of strongly induced mRNAs maintained polysomal levels under water deficit.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- PS levelse0
- Direction
- maintainede0
- Value
- Sixty per cente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sixty per cent of the dehydration-inducible mRNAs with twofold or greater increase in abundance maintained PS levels in response to water-deficit stress”
t0“Arabidopsis thaliana”
Claim · model-written summary
Forty percent of strongly induced mRNAs had impaired ribosome loading.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- ribosome loadinge0
- Direction
- impairede0
- Value
- 40%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while 40% showed impaired ribosome loading (RL)”
e1“dehydration-inducible mRNAs with twofold or greater increase in abundance”
t0“Arabidopsis thaliana”
Claim · model-written summary
Polysome association fell significantly for 92% of mRNAs whose abundance fell strongly.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- water-deficit stresse1
- Outcome
- PS associatione0
- Direction
- declinede0
- Value
- 92%e0
- Qualifier
- strong decrease in abundancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PS association declined significantly for 92% of the mRNAs that displayed a strong decrease in abundance”
e1“response to water-deficit stress”
t0“Arabidopsis thaliana”
Claim · model-written summary
Many mRNAs encoding proteins of similar function showed coordinate translation regulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- coordinate translational regulatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“many mRNAs that encode proteins of similar biological function displayed coordinate translational regulation”
t0“Arabidopsis thaliana”
Claim · model-written summary
The authors suggest polysomal mRNA abundance may estimate gene expression more accurately than total cellular mRNA.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- estimate of gene expressione0
- Direction
- more accuratee0
- Comparator
- total cellular mRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the abundance of PS mRNA may provide a more accurate estimate of gene expression than total cellular mRNA”
t0“Arabidopsis thaliana”
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