Abstract
Plant cell wall modification is a critical component in stress responses. Endo-1,4-β-glucanases (EGs) take part in cell wall editing processes, e.g. elongation, ripening and abscission. Here we studied the infection response of Solanum lycopersicum and Arabidopsis thaliana with impaired EGs. Transgenic TomCel1 and TomCel2 tomato antisense plants challenged with Pseudomonas syringae showed higher susceptibility, callose priming and increased jasmonic acid pathway marker gene expression. These two EGs could be resistance factors and may act as negative regulators of callose deposition, probably by interfering with the defence-signalling network. A study of a set of Arabidopsis EG T-DNA insertion mutants challenged with P. syringae and Botrytis cinerea revealed that the lack of other EGs interferes with infection phenotype, callose deposition, expression of signalling pathway marker genes and hormonal balance. We conclude that a lack of EGs could alter plant response to pathogens by modifying the properties of the cell wall and/or interfering with signalling pathways, contributing to generate the appropriate signalling outcomes. Analysis of microarray data demonstrates that EGs are differentially expressed upon many different plant-pathogen challenges, hormone treatments and many abiotic stresses. We found some Arabidopsis EG mutants with increased tolerance to osmotic and salt stress. Our results show that impairing EGs can alter plant-pathogen interactions and may contribute to appropriate signalling outcomes in many different biotic and abiotic plant stress responses.
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Claims 7
Claim · model-written summary
Tomato TomCel1 and TomCel2 antisense plants had higher susceptibility to Pseudomonas.
- Taxon
- tomato
Solanum lycopersicume0 - Material
- Transgenic TomCel1 and TomCel2 tomato antisense plantse0
- Treatment
- challenged with Pseudomonas syringaee0
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee0pathogen - Gene
- TomCel1 and TomCel2e0
- Outcome
- susceptibilitye0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic TomCel1 and TomCel2 tomato antisense plants challenged with Pseudomonas syringae showed higher susceptibility, callose priming and increased jasmonic acid pathway marker gene expression”
Claim · model-written summary
The challenged tomato antisense plants showed callose priming.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- Pseudomonas syringaePseudomonas syringaee0pathogen
- Outcome
- callose priminge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic TomCel1 and TomCel2 tomato antisense plants challenged with Pseudomonas syringae showed higher susceptibility, callose priming and increased jasmonic acid pathway marker gene expression”
Claim · model-written summary
The challenged tomato antisense plants increased jasmonic acid pathway marker gene expression.
- Taxon
- tomatoSolanum lycopersicume1
- Interactor
- Pseudomonas syringaePseudomonas syringaee2pathogene0
- Gene
- jasmonic acid pathway marker genee0
- Outcome
- jasmonic acid pathway marker gene expressione0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased jasmonic acid pathway marker gene expression”
e1“tomato antisense plants challenged with Pseudomonas syringae”
e2“Pseudomonas syringae”
Claim · model-written summary
Arabidopsis EG mutants altered infection phenotype and callose deposition.
- Taxon
- Arabidopsis
Arabidopsise1 - Interactor
- P. syringaePseudomonas syringaee2pathogene0
- Gene
- EGse0
- Outcome
- infection phenotype, callose depositione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lack of other EGs interferes with infection phenotype, callose deposition, expression of signalling pathway marker genes and hormonal balance”
e1“Arabidopsis EG T-DNA insertion mutants”
e2“P. syringae and Botrytis cinerea”
Claim · model-written summary
Arabidopsis EG mutants altered signalling gene expression and hormonal balance.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- EGse0
- Outcome
- expression of signalling pathway marker genes and hormonal balancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lack of other EGs interferes with infection phenotype, callose deposition, expression of signalling pathway marker genes and hormonal balance”
e1“Arabidopsis EG T-DNA insertion mutants”
Claim · model-written summary
EGs were differentially expressed under plant-pathogen, hormone and abiotic challenges.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- EGse0
- Outcome
- EGs are differentially expressede0
- Direction
- differentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EGs are differentially expressed upon many different plant-pathogen challenges, hormone treatments and many abiotic stresses”
e1“Arabidopsis EG T-DNA insertion mutants”
Claim · model-written summary
Some Arabidopsis EG mutants had increased osmotic and salt stress tolerance.
- Taxon
- ArabidopsisArabidopsise0
- Material
- Arabidopsis EG mutantse0
- Outcome
- tolerance to osmotic and salt stresse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some Arabidopsis EG mutants with increased tolerance to osmotic and salt stress”
The same questions in other papers
Pseudomonas syringae · disease · Solanum lycopersicum (tomato)Positive and negative results
2 in the opposite direction
- positive
- positive
infection · disease · Solanum lycopersicum (tomato)Positive and negative results
26 papers in the same direction as this paper · 29 in the opposite direction
- positive
- positive
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See all 56 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.