Abstract
TaLr35PR5 gene was obtained from the gDNA and cDNA of TcLr35 wheat. It was induced by Puccinia triticina, ABA and SA, but TaLr35PR5 was induced earlier and its expression level was higher in the incompatible interaction than that in the compatible interaction. In addition, the accumulations of TaLr35PR5 increased stably and showed significant peak challenged by P. triticina at different growth and development periods of TcLr35 wheat while it maintained similar level and changed little in mock inoculated. Western blottings were conducted to confirm that TaLr35PR5 be induced by P. triticina infection at the protein expression level. Similar to the expression patterns of TaLr35PR5 at RNA levels, the accumulations of TaLr35PR5 protein were weak in the seedling stage, then increased to the peak and kept constant levels at the mature stage which is consistent with the expression feature of Lr35 gene as an adult plant resistance gene.
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Claims 10
Claim · model-written summary
TaLr35PR5 was obtained from TcLr35 wheat genomic DNA and cDNA.
- Taxon
- wheat
Triticum aestivume0 - Material
- TcLr35 wheate0
- Gene
- TaLr35PR5e0
- Outcome
- gene was obtainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaLr35PR5 gene was obtained from the gDNA and cDNA of TcLr35 wheat.”
Claim · model-written summary
Puccinia triticina induced TaLr35PR5.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Puccinia triticinae0
- Interactor
- Puccinia triticina
Puccinia triticinae0pathogen - Gene
- TaLr35PR5e0
- Outcome
- TaLr35PR5e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was induced by Puccinia triticina, ABA and SA, but TaLr35PR5 was induced earlier and its expression level was higher in the incompatible interaction than that in the compatible interaction.”
t0“wheat”
Claim · model-written summary
ABA induced TaLr35PR5.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was induced by Puccinia triticina, ABA and SA, but TaLr35PR5 was induced earlier and its expression level was higher in the incompatible interaction than that in the compatible interaction.”
t0“wheat”
Claim · model-written summary
SA induced TaLr35PR5.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was induced by Puccinia triticina, ABA and SA, but TaLr35PR5 was induced earlier and its expression level was higher in the incompatible interaction than that in the compatible interaction.”
t0“wheat”
Claim · model-written summary
TaLr35PR5 induction was earlier in incompatible than compatible interactions.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- P. triticinaPuccinia triticinae1pathogen
- Gene
- TaLr35PR5e0
- Outcome
- TaLr35PR5 was inducede0
- Direction
- earliere0
- Comparator
- compatible interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaLr35PR5 was induced earlier and its expression level was higher in the incompatible interaction than that in the compatible interaction.”
t0“wheat”
e1“challenged by P. triticina at different growth and development periods of TcLr35 wheat”
Claim · model-written summary
TaLr35PR5 expression was higher in incompatible than compatible interactions.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaLr35PR5e0
- Outcome
- expression levele0
- Direction
- highere0
- Comparator
- compatible interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaLr35PR5 was induced earlier and its expression level was higher in the incompatible interaction than that in the compatible interaction.”
t0“wheat”
Claim · model-written summary
TaLr35PR5 accumulation increased and peaked after pathogen challenge at different wheat developmental periods.
- Taxon
- wheatTriticum aestivume0
- Material
- TcLr35 wheate0
- Treatment
- challenged by P. triticinae0
- Interactor
- P. triticinaPuccinia triticinae0pathogen
- Gene
- TaLr35PR5e0
- Outcome
- accumulations of TaLr35PR5e0
- Direction
- increased stablye0
- Comparator
- mock inoculatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulations of TaLr35PR5 increased stably and showed significant peak challenged by P. triticina at different growth and development periods of TcLr35 wheat while it maintained similar level and changed little in mock inoculated.”
Claim · model-written summary
Mock-inoculated TaLr35PR5 accumulation changed little.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- mock inoculatede0
- Outcome
- levele0
- Direction
- changed littlee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while it maintained similar level and changed little in mock inoculated.”
t0“wheat”
Claim · model-written summary
Western blotting confirmed TaLr35PR5 protein induction by P. triticina infection.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- P. triticina infectione0
- Interactor
- P. triticinaPuccinia triticinae0pathogen
- Gene
- TaLr35PR5e0
- Outcome
- protein expression levele0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Western blottings were conducted to confirm that TaLr35PR5 be induced by P. triticina infection at the protein expression level.”
t0“wheat”
Claim · model-written summary
TaLr35PR5 protein was weak in seedlings, rose to a peak, and remained constant at maturity.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- seedling stagee0
- Gene
- TaLr35PR5e0
- Outcome
- accumulations of TaLr35PR5 proteine0
- Direction
- increased to the peake0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulations of TaLr35PR5 protein were weak in the seedling stage, then increased to the peak and kept constant levels at the mature stage”
t0“wheat”
The same questions in other papers
Puccinia triticina · molecular: expression · Triticum aestivum (wheat)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
infection · molecular: expression · Triticum aestivum (wheat)Positive and negative results
29 papers in the same direction as this paper · 8 in the opposite direction
- no effect
- negative
- no effect
34 papers more in this group
See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.