Abstract
An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada. Response was highly dependent on the season, with above 69 % of culture developing adventitious shoots during spring. Greatest regeneration and multiplication was observed in modified Murashige and Skoog (MS) medium supplemented with 13.31 μM benzyladenine and 2.68 μM α-naphthalene acetic acid (NAA) in C. longa or 2.46 μM indolebutyric acid in C. amada. Effect of sugars and agar at different concentrations were also studied and 2 % maltose and 0.7 % agar were found optimum for shoot multiplication and regeneration. Most plantlets developed roots simultaneously but others formed roots when subcultured in 1/2 MS medium supplemented with 2.68 μM NAA. Plants were successfully hardened in greenhouse with 80 % survival. The genetic purity of micropropagated plantlets was analyzed using RAPD and protein profiles.
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Claims 8
Claim · model-written summary
Adventitious shoot development in Curcuma rhizome cultures was highly dependent on season, with above 69 % of cultures developing shoots during spring.
- Organ / stage
- rhizomese0
- Outcome
- developing adventitious shootse1
- Value
- above 69 %e1
- Qualifier
- highly dependent on the seasone1
- Setting
- during springe1
CurcumaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada”
e1“Response was highly dependent on the season, with above 69 % of culture developing adventitious shoots during spring”
Claim · model-written summary
In C. longa, the greatest regeneration and multiplication was obtained in modified MS medium with 13.31 μM benzyladenine and 2.68 μM NAA.
- Taxon
- C. longa
_W_IMG_2440.jpg?width=60)
Curcuma longae2 - Treatment
- 13.31 μM benzyladenine and 2.68 μM α-naphthalene acetic acid (NAA)e2
- Outcome
- regeneration and multiplicatione2
- Direction
- Greateste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_W_IMG_2440.jpg?width=500)
Verbatim
e2“Greatest regeneration and multiplication was observed in modified Murashige and Skoog (MS) medium supplemented with 13.31 μM benzyladenine and 2.68 μM α-naphthalene acetic acid (NAA) in C. longa”
Claim · model-written summary
In C. amada, the greatest regeneration and multiplication was obtained with 2.46 μM indolebutyric acid.
- Taxon
- C. amada
Curcuma amadae3 - Treatment
- 2.46 μM indolebutyric acide3
- Outcome
- regeneration and multiplicatione2
- Direction
- Greateste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Greatest regeneration and multiplication was observed in modified Murashige and Skoog (MS) medium supplemented with 13.31 μM benzyladenine and 2.68 μM α-naphthalene acetic acid (NAA) in C. longa”
e3“2.46 μM indolebutyric acid in C. amada”
Claim · model-written summary
The optimum sugar for shoot multiplication and regeneration was 2 % maltose.
- Treatment
- 2 % maltosee4
- Outcome
- shoot multiplication and regeneratione4
- Direction
- optimume4
- Comparator
- different concentrationse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada”
e4“2 % maltose and 0.7 % agar were found optimum for shoot multiplication and regeneration”
e5“Effect of sugars and agar at different concentrations were also studied”
Claim · model-written summary
The optimum agar concentration for shoot multiplication and regeneration was 0.7 %.
- Treatment
- 0.7 % agare4
- Outcome
- shoot multiplication and regeneratione4
- Direction
- optimume4
- Comparator
- different concentrationse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada”
e4“2 % maltose and 0.7 % agar were found optimum for shoot multiplication and regeneration”
e5“Effect of sugars and agar at different concentrations were also studied”
Claim · model-written summary
Plantlets subcultured in 1/2 MS medium with 2.68 μM NAA formed roots.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada”
e6“Most plantlets developed roots simultaneously but others formed roots when subcultured in 1/2 MS medium supplemented with 2.68 μM NAA”
Claim · model-written summary
Most Curcuma plantlets developed roots simultaneously.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada”
e6“Most plantlets developed roots simultaneously but others formed roots when subcultured in 1/2 MS medium supplemented with 2.68 μM NAA”
Claim · model-written summary
Curcuma plantlets hardened in the greenhouse had 80 % survival.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An efficient plant regeneration protocol was developed from rhizomes of two Curcuma species C. longa and C. amada”
e7“Plants were successfully hardened in greenhouse with 80 % survival”
The same questions in other papers
1-naphthaleneacetic acid · pest · Curcuma longa (turmeric)Results agree
2 papers in the same direction as this paper
- positive
- positive
N-benzyladenine · pest · Curcuma longa (turmeric)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.