Abstract
Callus induction, organ formation from callus and single callus cell culture have been tried in wheat. Though kinetin showed no effects, supplements of 2,4-D (1~10mg/1) or IAA (50mg/1) to the basal media induced calluses from seedling roots of einkorn, emmer and common wheats, and from stem pieces of common wheat. The best callus growth was obtained when casein hydrolysate (1g/1) or coconut milk (1%) was added to the basal media. Callus growth was also vigorous when 2,4-D (0.5~2.0mg/1) was added. Root formation from callus took place in all kinds of tested media, except those containing no growth factors or supplemented with 2,4-D at high concentrations (1~5mg/1). Shoot formation occurred in six cases and no growth factors were found to be specifically effective on shoot differentiation. Two plants were restored and reached maturity. Calluses of common wheat consisted of eudiploid and aneuploid cells at almost the same frequencies. The great majority of aneuploid cells had 42 ± 3 chromosomes. The restored plants showed normal chromosome constitution. Single callus-cell suspensions were obtained by the liquid culture of seeds in a shaker. A filtrate of the single cell suspension was plated on a solid agar medium, and some colonies were formed. However, plating efficiency was very low and colony growth was slow.
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Claims 12
Claim · model-written summary
2,4-D or IAA induced callus from wheat seedling roots and common wheat stems.
- Taxon
- common wheat
Triticum aestivume0 - Organ / stage
- seedling rootse0
- Treatment
- 2,4-D (1~10mg/1) or IAA (50mg/1)e0
- Outcome
- callusese0
- Direction
- inducede0
- Comparator
- basal mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“supplements of 2,4-D (1~10mg/1) or IAA (50mg/1) to the basal media induced calluses from seedling roots of einkorn, emmer and common wheats, and from stem pieces of common wheat.”
Claim · model-written summary
Kinetin showed no effect on wheat callus induction.
- Taxon
- WHEATTriticum aestivumt0
- Treatment
- kinetine0
- Outcome
- callusese0
- Direction
- no effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Though kinetin showed no effects, supplements of 2,4-D (1~10mg/1) or IAA (50mg/1) to the basal media induced calluses from seedling roots of einkorn, emmer and common wheats”
t0“WHEAT TISSUES”
Claim · model-written summary
Casein hydrolysate or coconut milk yielded the best wheat callus growth.
- Taxon
- WHEATTriticum aestivumt0
- Treatment
- casein hydrolysate (1g/1) or coconut milk (1%)e0
- Outcome
- callus growthe0
- Direction
- beste0
- Comparator
- basal mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best callus growth was obtained when casein hydrolysate (1g/1) or coconut milk (1%) was added to the basal media.”
t0“WHEAT TISSUES”
Claim · model-written summary
2,4-D at 0.5~2.0mg/1 supported vigorous wheat callus growth.
- Taxon
- WHEATTriticum aestivumt0
- Treatment
- 2,4-D (0.5~2.0mg/1)e0
- Outcome
- Callus growthe0
- Direction
- vigorouse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus growth was also vigorous when 2,4-D (0.5~2.0mg/1) was added.”
t0“WHEAT TISSUES”
Claim · model-written summary
Wheat callus formed roots in all tested media except those lacking growth factors or containing high 2,4-D.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- Root formation from calluse0
- Qualifier
- except those containing no growth factors or supplemented with 2,4-D at high concentrations (1~5mg/1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root formation from callus took place in all kinds of tested media, except those containing no growth factors or supplemented with 2,4-D at high concentrations (1~5mg/1).”
t0“WHEAT TISSUES”
Claim · model-written summary
Wheat callus formed shoots in six cases, without a specifically effective growth factor.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- Shoot formatione0
- Value
- six casese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot formation occurred in six cases and no growth factors were found to be specifically effective on shoot differentiation.”
t0“WHEAT TISSUES”
Claim · model-written summary
Two regenerated wheat plants reached maturity.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- plants were restored and reached maturitye0
- Value
- Two plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two plants were restored and reached maturity.”
t0“WHEAT TISSUES”
Claim · model-written summary
Common wheat callus had eudiploid and aneuploid cells at nearly equal frequencies.
- Taxon
- common wheatTriticum aestivume0
- Outcome
- eudiploid and aneuploid cellse0
- Qualifier
- almost the same frequenciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calluses of common wheat consisted of eudiploid and aneuploid cells at almost the same frequencies.”
Claim · model-written summary
Most aneuploid wheat callus cells had 42 ± 3 chromosomes.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- aneuploid cellse0
- Value
- 42 ± 3 chromosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The great majority of aneuploid cells had 42 ± 3 chromosomes.”
t0“WHEAT TISSUES”
Claim · model-written summary
Regenerated wheat plants had normal chromosome constitution.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- normal chromosome constitutione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The restored plants showed normal chromosome constitution.”
t0“WHEAT TISSUES”
Claim · model-written summary
Liquid culture produced single wheat callus-cell suspensions and plated cells formed some colonies.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- some colonies were formede0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Single callus-cell suspensions were obtained by the liquid culture of seeds in a shaker. A filtrate of the single cell suspension was plated on a solid agar medium, and some colonies were formed.”
t0“WHEAT TISSUES”
Claim · model-written summary
Single wheat callus cell plating efficiency was very low and colony growth slow.
- Taxon
- WHEATTriticum aestivumt0
- Outcome
- plating efficiencye0
- Direction
- very lowe0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, plating efficiency was very low and colony growth was slow.”
t0“WHEAT TISSUES”
The same questions in other papers
2,4-D · growth · Triticum aestivum (wheat)Results differ
34 papers in the same direction as this paper · 14 in the opposite direction · 2 reporting no difference from an alternative
- negative
- negative
- negative
47 papers more in this group
See all 51 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.