Abstract
Treatment of barley seeds ( Hordeum vulgare L.) with streptomycin, an inhibitor of plastid protein synthesis, resulted in growth of the albino phenotype seedlings with ribosome-deficient undifferentiated plastids and chlorophyll (Chl) level as low as 0.1% of that in control plant leaves. A major effect of the antibiotic was almost complete suppression of the ability of plants to synthesize 5-aminolevulinic acid (ALA) intended for Chl biosynthesis. The activity of synthesis of ALA intended for heme porphyrin biosynthesis in etiolated and greening seedlings and in light-grown albinophenotype plants was insensitive to light and cytokinins. In the upper parts of leaves of streptomycin-treated plants, exhibiting 60% Chl deficit, the cells with three types of chloroplasts could be observed: normally developed chloroplasts, chloroplasts composed of single thylakoids and grana, and completely undifferentiated plastids. In this Chl-deficient tissue, ALA synthesis was found to be stimulated by kinetin but much less than in leaves of the control plants. The endogenous cytokinin content in etiolated and greening seedlings treated with streptomycin was almost the same as it was in untreated control seedlings. The cytokinin level in the white tissue of plants grown in the light was on average twice as high as that in green leaves of the control plants. The capability of kinetin to stimulate the synthesis of ALA used for Chl biosynthesis was found to correlate with the Chl content and organization of the chloroplast internal structure. This correlation confirms the hypothesis that the normally developed internal structure of plastids is essential for the adequate phytohormone response in plants.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Streptomycin produced albino seedlings with deficient plastids.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- streptomycine0
- Outcome
- albino phenotype seedlings with ribosome-deficient undifferentiated plastidse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“barley seedlings”
e0“Treatment of barley seeds ( Hordeum vulgare L.) with streptomycin, an inhibitor of plastid protein synthesis, resulted in growth of the albino phenotype seedlings with ribosome-deficient undifferentiated plastids”
Claim · model-written summary
Streptomycin-treated seedlings had chlorophyll at 0.1% of controls.
- Taxon
- barley seedlingsHordeum vulgaret0
- Treatment
- streptomycine1
- Outcome
- chlorophyll (Chl) levele0
- Direction
- as low ase0
- Value
- 0.1%e0
- Comparator
- control plant leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley seedlings”
e0“chlorophyll (Chl) level as low as 0.1% of that in control plant leaves.”
e1“Treatment of barley seeds ( Hordeum vulgare L.) with streptomycin”
Claim · model-written summary
Streptomycin almost completely suppressed ALA synthesis for chlorophyll biosynthesis.
- Taxon
- barley seedlingsHordeum vulgaret0
- Treatment
- antibiotice0
- Outcome
- ability of plants to synthesize 5-aminolevulinic acid (ALA) intended for Chl biosynthesise0
- Direction
- suppressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley seedlings”
e0“A major effect of the antibiotic was almost complete suppression of the ability of plants to synthesize 5-aminolevulinic acid (ALA) intended for Chl biosynthesis.”
Claim · model-written summary
ALA synthesis for heme porphyrin was insensitive to light and cytokinins.
- Taxon
- barley seedlingsHordeum vulgaret0
- Treatment
- light and cytokininse0
- Outcome
- activity of synthesis of ALA intended for heme porphyrin biosynthesise0
- Direction
- insensitivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley seedlings”
e0“The activity of synthesis of ALA intended for heme porphyrin biosynthesis in etiolated and greening seedlings and in light-grown albinophenotype plants was insensitive to light and cytokinins.”
Claim · model-written summary
Streptomycin-treated upper leaves with 60% chlorophyll deficit contained three chloroplast types.
- Taxon
- barley seedlingsHordeum vulgaret0
- Organ / stage
- upper parts of leavese0
- Outcome
- three types of chloroplastse0
- Value
- 60% Chl deficite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley seedlings”
e0“In the upper parts of leaves of streptomycin-treated plants, exhibiting 60% Chl deficit, the cells with three types of chloroplasts could be observed: normally developed chloroplasts, chloroplasts composed of single thylakoids and grana, and completely undifferentiated plastids.”
Claim · model-written summary
Kinetin stimulated ALA synthesis in chlorophyll-deficient tissue, less than in control leaves.
- Taxon
- barley seedlingsHordeum vulgaret0
- Treatment
- kinetine0
- Outcome
- ALA synthesise0
- Direction
- stimulatede0
- Qualifier
- much lesse0
- Comparator
- leaves of the control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley seedlings”
e0“In this Chl-deficient tissue, ALA synthesis was found to be stimulated by kinetin but much less than in leaves of the control plants.”
Claim · model-written summary
Cytokinin content in etiolated and greening treated seedlings was almost the same as untreated controls.
- Taxon
- barley seedlingsHordeum vulgaret0
- Treatment
- streptomycine0
- Outcome
- endogenous cytokinin contente0
- Direction
- almost the samee0
- Comparator
- untreated control seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley seedlings”
e0“The endogenous cytokinin content in etiolated and greening seedlings treated with streptomycin was almost the same as it was in untreated control seedlings.”
Claim · model-written summary
Cytokinin level in white light-grown tissue averaged twice that of green control leaves.
- Taxon
- barley seedlingsHordeum vulgaret0
- Organ / stage
- white tissuee0
- Outcome
- cytokinin levele0
- Direction
- twice as highe0
- Value
- twicee0
- Comparator
- green leaves of the control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley seedlings”
e0“The cytokinin level in the white tissue of plants grown in the light was on average twice as high as that in green leaves of the control plants.”
Claim · model-written summary
Kinetin stimulation of ALA synthesis correlated with chlorophyll content and chloroplast organization.
- Taxon
- barley seedlingsHordeum vulgaret0
- Treatment
- kinetine0
- Outcome
- capability of kinetin to stimulate the synthesis of ALAe0
- Qualifier
- Chl content and organization of the chloroplast internal structuree0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley seedlings”
e0“The capability of kinetin to stimulate the synthesis of ALA used for Chl biosynthesis was found to correlate with the Chl content and organization of the chloroplast internal structure.”
Claim · model-written summary
Authors infer normally developed plastid structure is essential for adequate phytohormone response.
- Taxon
- barley seedlingsHordeum vulgaret0
- Outcome
- adequate phytohormone responsee0
- Qualifier
- normally developed internal structure of plastidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“barley seedlings”
e0“the normally developed internal structure of plastids is essential for the adequate phytohormone response in plants.”
The same questions in other papers
kinetin · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
2 papers more in this group
See all 6 papers →antimicrobial agent · molecular: metabolites · Hordeum vulgare (barley)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.