Abstract
We have examined the time course for accumulation of each of 12 different nuclear gene transcripts in pea buds after irradiating dark grown seedlings with a single pulse low fluence red light (10(3) micromoles per square meter delivered in 100 seconds). The 12 time courses can be grouped into four general classes. Six transcripts (including RNAs coding for the chlorophyll a/b binding protein and ribulose-1,5-bisphosphate carboxylase) accumulate at a linear rate during 24 hours in darkness following the light pulse. Two transcripts increase rapidly at first but then reach a plateau after 3 hours and remain at that level for the next 21 hours. Another two transcripts exhibit a prolonged lag period before beginning to accumulate, and do not reach significant accumulation rates until 12 to 16 hours after the red light pulse. One transcript appears to undergo a transient increase in abundance in response to red light, but this is superimposed on a background of slowly increasing abundance of this RNA in control plants. This response, unlike all the others, exhibits reciprocity failure in experiments in which the same fluence of light is given over periods ranging between 50 and 4000 seconds.We have also examined the kinetics with which each of these 12 responses escapes from phytochrome-far-red absorbing form control by attempting to reverse the induction with far-red light given at various times after the red light pulse. Again, several different patterns are apparent for the different transcripts. The time at which far red reversibility first begins to be lost, the rate at which it is lost, and the final extent of reversibility remaining after 7 hours in the dark all differ for different transcripts. In addition, we have observed that some responses retain virtually complete photoreversibility for at least 7 hours. In some cases, a comparison of the time course and escape kinetic data indicates that relatively rapid turnover of the RNA must occur. It is not clear whether or not the rate of turnover is influenced by phytochrome.
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Claims 8
Claim · model-written summary
Six pea bud transcripts accumulated linearly for 24 hours after red light.
- Taxon
- Pea
Lathyrus oleraceust0 - Organ / stage
- Budst0
- Outcome
- transcriptse0
- Direction
- accumulatee0
- Value
- 24 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Developing Pea Buds”
e0“Six transcripts (including RNAs coding for the chlorophyll a/b binding protein and ribulose-1,5-bisphosphate carboxylase) accumulate at a linear rate during 24 hours in darkness following the light pulse.”
Claim · model-written summary
Two pea bud transcripts rose rapidly then plateaued after three hours.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- transcriptse0
- Direction
- increasee0
- Value
- 3 hourse0
- Qualifier
- remain at that level for the next 21 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Developing Pea Buds”
e0“Two transcripts increase rapidly at first but then reach a plateau after 3 hours and remain at that level for the next 21 hours.”
Claim · model-written summary
Two pea bud transcripts showed a lag and did not accumulate significantly until 12 to 16 hours.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- transcriptse0
- Direction
- accumulatee0
- Value
- 12 to 16 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Developing Pea Buds”
e0“Another two transcripts exhibit a prolonged lag period before beginning to accumulate, and do not reach significant accumulation rates until 12 to 16 hours after the red light pulse.”
Claim · model-written summary
One pea bud transcript showed a transient red-light increase against slowly rising control abundance.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- red lighte0
- Outcome
- abundancee0
- Direction
- increasee0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Developing Pea Buds”
e0“One transcript appears to undergo a transient increase in abundance in response to red light, but this is superimposed on a background of slowly increasing abundance of this RNA in control plants.”
Claim · model-written summary
The transient pea bud transcript response showed reciprocity failure over 50 to 4000 second light deliveries.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- reciprocity failuree0
- Qualifier
- between 50 and 4000 secondse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Developing Pea Buds”
e0“This response, unlike all the others, exhibits reciprocity failure in experiments in which the same fluence of light is given over periods ranging between 50 and 4000 seconds.”
Claim · model-written summary
Pea bud transcripts differed in the onset, rate and extent of loss of far-red reversibility.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- far red reversibilitye0
- Direction
- differe0
- Qualifier
- after 7 hours in the darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Developing Pea Buds”
e0“The time at which far red reversibility first begins to be lost, the rate at which it is lost, and the final extent of reversibility remaining after 7 hours in the dark all differ for different transcripts.”
Claim · model-written summary
Some pea bud transcript responses retained virtually complete photoreversibility for at least seven hours.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- virtually complete photoreversibilitye0
- Value
- 7 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Developing Pea Buds”
e0“some responses retain virtually complete photoreversibility for at least 7 hours”
Claim · model-written summary
Comparison of time courses and escape kinetics implied relatively rapid RNA turnover in some pea bud transcripts.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- relatively rapid turnover of the RNAe0
- Qualifier
- comparison of the time course and escape kinetic datae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Developing Pea Buds”
e0“In some cases, a comparison of the time course and escape kinetic data indicates that relatively rapid turnover of the RNA must occur.”
The same questions in other papers
light · pest · Lathyrus oleraceus (pea)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.