Abstract
Abstract The use of biotechnological methods is of particular interest in fruit tree crops since conventional breeding programs require long time and high resources before having impact upon commercialization. To develop an efficient plant regeneration protocol for pear rootstock Pyrodwarf, different combinations of two hormones, naphthalene-1-acetic acid (NAA) and Thidiazuron (TDZ) were studied on regeneration process. Other factors were also studied, such as darkness period and the use of different hormone concentrations during induction and expression phase. Our experiments showed that the length of darkness did not affect in vitro regeneration. Different concentrations of TDZ have different effects on regeneration: 6 μM has been shown to be more effective than 2.5 μM. A higher level of TDZ during induction period was the only important factor to induce regeneration independently to the presence of hormones during expression phase. The two hormones showed to be uneffective to induce a sufficient number of regenerating shoots from leaf explants of another pear rootstock, Fox11. Variability during regeneration was assessed using SSR markers. An identical genetic profile was observed between in vitro explants and regenerating shoots. These results are useful to include Pyrodwarf in genetic engineering experiments for breeding purposes. On the contrary, other hormones have to be evaluated for their effectiveness to induce in vitro regeneration in Fox11 leaf explants.
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Claims 6
Claim · model-written summary
Darkness duration did not affect in vitro regeneration.
- Taxon
- pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communist0 - Treatment
- length of darknesse0
- Outcome
- in vitro regeneratione0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“the length of darkness did not affect in vitro regeneration”
t0“pear”
Claim · model-written summary
TDZ at 6 μM regenerated more effectively than 2.5 μM.
- Taxon
- pearPyrus communist0
- Treatment
- 6 μMe0
- Outcome
- more effectivee0
- Direction
- more effectivee0
- Value
- 6 μMe0
- Comparator
- 2.5 μMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6 μM has been shown to be more effective than 2.5 μM”
t0“pear rootstock Pyrodwarf and assessment of SSR variability in regenerating shoots”
Claim · model-written summary
Higher TDZ during induction was the only important regeneration factor, independent of hormones in expression.
- Taxon
- pearPyrus communist0
- Treatment
- higher level of TDZ during induction periode0
- Outcome
- induce regeneratione0
- Direction
- highere0
- Qualifier
- independently to the presence of hormones during expression phasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A higher level of TDZ during induction period was the only important factor to induce regeneration independently to the presence of hormones during expression phase”
t0“pear”
Claim · model-written summary
NAA and TDZ did not induce enough shoots from Fox11 leaf explants.
- Taxon
- pearPyrus communise0
- Material
- Fox11e0
- Organ / stage
- leaf explantse0
- Treatment
- two hormonese0
- Outcome
- regenerating shootse0
- Direction
- uneffectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The two hormones showed to be uneffective to induce a sufficient number of regenerating shoots from leaf explants of another pear rootstock, Fox11”
t0“pear”
Claim · model-written summary
Explants and regenerating shoots had identical SSR profiles.
- Taxon
- pearPyrus communist0
- Outcome
- identical genetic profilee0
- Comparator
- in vitro explants and regenerating shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An identical genetic profile was observed between in vitro explants and regenerating shoots”
t0“pear”
Claim · model-written summary
The authors recommend evaluating other hormones for Fox11 regeneration.
- Taxon
- pearPyrus communist0
- Outcome
- other hormones have to be evaluated for their effectiveness to induce in vitro regeneration in Fox11 leaf explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“other hormones have to be evaluated for their effectiveness to induce in vitro regeneration in Fox11 leaf explants”
t0“pear”
The same questions in other papers
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.