Abstract
Rhizosphere soil was sampled in monocultures of Lolium perenne and Trifolium repens in June and October 2002, at two different nitrogen fertilisation levels (14 and 56 g N m(-2) year(-1)) and under two pCO(2) atmospheres (360 and 600 ppmv) at the Swiss FACE (Free Air Carbon dioxide Enrichment) site. Directly extracted soil DNA was analysed with restriction fragment length polymorphism (PCR-RFLP) by use of degenerated primers for the narG gene encoding the active site of the membrane-bound nitrate reductase. The corresponding enzyme activity of the nitrate reductase was determined colorimetrically after 24 h of anaerobic incubation. The narG PCR-RFLP fingerprints showed that the structure of the nitrate-reducing community was primarily affected by season and pH of the sampling site, whereas CO(2) enrichment, plant species or fertiliser treatment had no apparent effect. In contrast, the nitrate reductase activity responded to N fertilisation, CO(2) enrichment and plant species in October, whereas in June drought stress most likely kept the enzyme activity at a low level in all treatments. Apparently, the respiratory nitrate-reducing community adapted to different treatments primarily by altered enzyme activity.
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Claims 7
Claim · model-written summary
Season and pH of the sampling site primarily affected the structure of the nitrate-reducing community in the rhizosphere.
- Taxon
- Lolium perenne and Trifolium repens
Trifolium repens
Lolium perennee1 - Outcome
- structure of the nitrate-reducing communitye2
- Setting
- sampling sitee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rhizosphere soil was sampled in monocultures of Lolium perenne and Trifolium repens in June and October 2002”
e2“the structure of the nitrate-reducing community was primarily affected by season and pH of the sampling site, whereas CO(2) enrichment, plant species or fertiliser treatment had no apparent effect”
Claim · model-written summary
Elevated CO2, plant species and fertiliser treatment had no apparent effect on the structure of the nitrate-reducing community.
- Taxon
- Lolium perenne and Trifolium repensTrifolium repensLolium perennee1
- Treatment
- CO(2) enrichment, plant species or fertiliser treatmente2
- Outcome
- structure of the nitrate-reducing communitye2
- Direction
- no apparent effecte2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rhizosphere soil was sampled in monocultures of Lolium perenne and Trifolium repens in June and October 2002”
e2“the structure of the nitrate-reducing community was primarily affected by season and pH of the sampling site, whereas CO(2) enrichment, plant species or fertiliser treatment had no apparent effect”
Claim · model-written summary
Nitrate reductase activity responded to N fertilisation in October.
- Taxon
- Lolium perenne and Trifolium repensTrifolium repensLolium perennee1
- Treatment
- N fertilisatione3
- Outcome
- nitrate reductase activitye3
- Qualifier
- in Octobere3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rhizosphere soil was sampled in monocultures of Lolium perenne and Trifolium repens in June and October 2002”
e3“In contrast, the nitrate reductase activity responded to N fertilisation, CO(2) enrichment and plant species in October”
Claim · model-written summary
Elevated CO2 enrichment was associated with changed nitrate reductase activity in October.
- Taxon
- Lolium perenne and Trifolium repensTrifolium repensLolium perennee1
- Treatment
- CO(2) enrichmente3
- Outcome
- nitrate reductase activitye3
- Qualifier
- in Octobere3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rhizosphere soil was sampled in monocultures of Lolium perenne and Trifolium repens in June and October 2002”
e3“In contrast, the nitrate reductase activity responded to N fertilisation, CO(2) enrichment and plant species in October”
Claim · model-written summary
Plant species was associated with changed nitrate reductase activity in October.
- Taxon
- Lolium perenne and Trifolium repensTrifolium repensLolium perennee1
- Treatment
- plant speciese3
- Outcome
- nitrate reductase activitye3
- Qualifier
- in Octobere3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rhizosphere soil was sampled in monocultures of Lolium perenne and Trifolium repens in June and October 2002”
e3“In contrast, the nitrate reductase activity responded to N fertilisation, CO(2) enrichment and plant species in October”
Claim · model-written summary
Drought stress most likely kept nitrate reductase activity at a low level in all treatments in June.
- Taxon
- Lolium perenne and Trifolium repensTrifolium repensLolium perennee1
- Treatment
- drought stresse4
- Outcome
- enzyme activitye4
- Qualifier
- at a low levele4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rhizosphere soil was sampled in monocultures of Lolium perenne and Trifolium repens in June and October 2002”
e4“in June drought stress most likely kept the enzyme activity at a low level in all treatments”
Claim · model-written summary
The respiratory nitrate-reducing community adapted to the treatments primarily by altered enzyme activity.
- Taxon
- Lolium perenne and Trifolium repensTrifolium repensLolium perennee1
- Outcome
- adapted to different treatmentse5
- Qualifier
- altered enzyme activitye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rhizosphere soil was sampled in monocultures of Lolium perenne and Trifolium repens in June and October 2002”
e5“Apparently, the respiratory nitrate-reducing community adapted to different treatments primarily by altered enzyme activity”
The same questions in other papers
co2 · nutrient: content · Trifolium repens (white clover)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
co2 · nutrient: content · Lolium perenne (perennial ryegrass)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
fertilization · nutrient: content · Trifolium repens (white clover)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →fertilization · nutrient: content · Lolium perenne (perennial ryegrass)Positive and negative results
4 papers in the same direction as this paper · 12 in the opposite direction
- positive
- positive
- positive
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.