Abstract
Production of microspore-derived embryos from cultured anthers is now a well-established technique for the isolation of homozygous lines in sunflower. Experiment was conducted to optimize the culture composition for wild Helianthus and inerspecific hybrids. Anthers of uninucleate microspores were cultured in basal MS media with different hormonal combinations. The induction of callus was quick and proliferation high with the presence of 2,4-D, low cytokinin and auxin contents. Excess cytokinin and auxin had no significance in callus induction. Regeneration potential increased with the increased/optimum amount of casein hydrolysate and benzyl amino purine. Kinetin had no specific influence of regeneration frequency of callus. Genotype had a significant effect on the capacity of callus induction and plantlet formation. H.annuus × H.tuberosus responded well and produced plantlets in optimum culture medium. Androgenesis of interspecific hybrid H.annuus × H.tuberosus formed plantlets that surpassed the embryoid stage.
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Claims 7
Claim · model-written summary
Callus induction from sunflower anthers was quick and proliferation high with 2,4-D and low cytokinin and auxin contents.
- Taxon
- sunflower
Helianthus annuuse1 - Treatment
- 2,4-D, low cytokinin and auxin contentse4
- Outcome
- induction of calluse4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“isolation of homozygous lines in sunflower”
e4“The induction of callus was quick and proliferation high with the presence of 2,4-D, low cytokinin and auxin contents”
Claim · model-written summary
Excess cytokinin and auxin had no significant effect on callus induction.
- Taxon
- sunflowerHelianthus annuuse1
- Treatment
- Excess cytokinin and auxine5
- Outcome
- callus inductione5
- Direction
- no_changeinferrede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“isolation of homozygous lines in sunflower”
e5“Excess cytokinin and auxin had no significance in callus induction”
Claim · model-written summary
Regeneration potential increased with increased or optimum casein hydrolysate and benzyl amino purine.
- Taxon
- sunflowerHelianthus annuuse1
- Treatment
- casein hydrolysate and benzyl amino purinee6
- Outcome
- Regeneration potentiale6
- Direction
- increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“isolation of homozygous lines in sunflower”
e6“Regeneration potential increased with the increased/optimum amount of casein hydrolysate and benzyl amino purine”
Claim · model-written summary
Kinetin had no specific influence on the regeneration frequency of callus.
- Taxon
- sunflowerHelianthus annuuse1
- Treatment
- Kinetine7
- Outcome
- regeneration frequency of calluse7
- Direction
- no_changeinferrede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“isolation of homozygous lines in sunflower”
e7“Kinetin had no specific influence of regeneration frequency of callus”
Claim · model-written summary
Genotype had a significant effect on the capacity of callus induction and plantlet formation.
- Taxon
- sunflowerHelianthus annuuse1
- Material
- Genotypee2
- Outcome
- capacity of callus induction and plantlet formatione2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“isolation of homozygous lines in sunflower”
e2“Genotype had a significant effect on the capacity of callus induction and plantlet formation”
Claim · model-written summary
The interspecific hybrid H.annuus × H.tuberosus responded well and produced plantlets in optimum culture medium.
- Taxon
- sunflowerHelianthus annuuse1
- Material
- H.annuus × H.tuberosuse2
- Treatment
- optimum culture mediume2
- Outcome
- produced plantletse2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“isolation of homozygous lines in sunflower”
e2“H.annuus × H.tuberosus responded well and produced plantlets in optimum culture medium”
Claim · model-written summary
Androgenesis of the interspecific hybrid H.annuus × H.tuberosus formed plantlets that surpassed the embryoid stage.
- Taxon
- sunflowerHelianthus annuuse1
- Material
- H.annuus × H.tuberosuse2
- Organ / stage
- embryoid stagee2
- Outcome
- formed plantlets that surpassed the embryoid stagee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“isolation of homozygous lines in sunflower”
e2“Androgenesis of interspecific hybrid H.annuus × H.tuberosus formed plantlets that surpassed the embryoid stage”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.