Abstract
Flow-cytometric analysis was carried out to determine the effect of low temperature on the changes in proportion of 2C:4C complement of DNA in root cells from three different zones (meristem apex, elongation and differentiation) in seedlings of Medicago sativa cv. Sitel. 2C:4C complement of DNA in cells were estimated after the treatments of the roots at control temperature of24°C, elevated temperatures of26°C, 30°C, 37°C and low temperatures—10°C and 5°C (in three different periods of duration 15, 38, 45 days). The observed results showed that at control temperature percentage of nuclei 2C:4C in meristem apex is 65:35, in elongation zone 50:50 and in differentiation zone is 40:60. The elevated temperatures (26°C, 30°C, 37°C) influenced this proportion very slightly while treatments of roots with low temperature especially in a period of 45 days changed 2C:4C complement of DNA drastically. It was found that root treated for 45 days at temperature of 10°C the 2C:4C in meristem apex is 78:22, elongation zone 74:26 and in differentiation zone is 70:30. In the case of temperature of 5°C it was 85:15 in meristem apex, 80:20 in elongation zone and 71:29 in differentiation zone.In order to visualize the impact of low temperature on cell cycle plasticity with decrease of dividing cells, roots of transgenic Medicago falcata plants were exposed at control (24°C) and low (5°C) temperatures for the period of 15 and 45 days. These plants carried gus gene under the promoters (cycl, cyc3a)from cell cycle genes After this temperature treatment roots cells were activated for division with different treatments for 1hour. GUS assay were performed for control and pre-treated root tips. The expression of marker gene was observed in highly divided cells of pre-treated root at control temperature. GUS gene was not expressed in pre-treated roots exposed to low temperature for 45 days.
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Claims 12
Claim · model-written summary
At control temperature, meristem apex nuclei had a 2C:4C proportion of 65:35.
- Taxon
- Medicago sativa
Medicago sativae1 - Material
- Sitele1
- Organ / stage
- meristem apexe0
- Treatment
- control temperaturee0
- Outcome
- percentage of nuclei 2C:4Ce0
- Value
- 65:35e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at control temperature percentage of nuclei 2C:4C in meristem apex is 65:35”
e1“Medicago sativa cv. Sitel”
Claim · model-written summary
At control temperature, elongation zone nuclei had a 2C:4C proportion of 50:50.
- Taxon
- Medicago sativaMedicago sativae2
- Material
- Sitele2
- Organ / stage
- elongation zonee1
- Treatment
- control temperaturee1
- Outcome
- percentage of nuclei 2C:4Ce1
- Value
- 50:50e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in elongation zone 50:50”
e1“at control temperature percentage of nuclei 2C:4C in meristem apex is 65:35, in elongation zone 50:50”
e2“Medicago sativa cv. Sitel”
Claim · model-written summary
At control temperature, differentiation zone nuclei had a 2C:4C proportion of 40:60.
- Taxon
- Medicago sativaMedicago sativae1
- Material
- Sitele1
- Organ / stage
- differentiation zonee0
- Treatment
- control temperaturee0
- Outcome
- percentage of nuclei 2C:4Ce0
- Value
- 40:60e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at control temperature percentage of nuclei 2C:4C in meristem apex is 65:35, in elongation zone 50:50 and in differentiation zone is 40:60”
e1“Medicago sativa cv. Sitel”
Claim · model-written summary
Elevated temperatures changed the 2C:4C nuclear proportion only slightly.
- Taxon
- Medicago sativaMedicago sativae1
- Treatment
- elevated temperatures (26°C, 30°C, 37°C)e0
- Outcome
- this proportione0
- Direction
- very slightlye0
- Comparator
- control temperature of24°Ce2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The elevated temperatures (26°C, 30°C, 37°C) influenced this proportion very slightly”
e1“Medicago sativa cv. Sitel”
e2“control temperature of24°C”
Claim · model-written summary
After 45 days at 10°C, meristem apex 2C:4C proportion was 78:22.
- Taxon
- Medicago sativaMedicago sativae1
- Organ / stage
- meristem apexe0
- Treatment
- 45 days at temperature of 10°Ce0
- Outcome
- 2C:4C in meristem apexe0
- Value
- 78:22e0
- Comparator
- control temperaturee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root treated for 45 days at temperature of 10°C the 2C:4C in meristem apex is 78:22, elongation zone 74:26 and in differentiation zone is 70:30”
e1“Medicago sativa cv. Sitel”
e2“control temperature percentage of nuclei 2C:4C in meristem apex is 65:35”
Claim · model-written summary
After 45 days at 10°C, elongation zone 2C:4C proportion was 74:26.
- Taxon
- Medicago sativaMedicago sativae1
- Organ / stage
- elongation zonee0
- Treatment
- 45 days at temperature of 10°Ce0
- Outcome
- 2C:4Ce0
- Value
- 74:26e0
- Comparator
- control temperaturee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root treated for 45 days at temperature of 10°C the 2C:4C in meristem apex is 78:22, elongation zone 74:26 and in differentiation zone is 70:30”
e1“Medicago sativa cv. Sitel”
e2“control temperature percentage of nuclei 2C:4C in meristem apex is 65:35, in elongation zone 50:50”
Claim · model-written summary
After 45 days at 10°C, differentiation zone 2C:4C proportion was 70:30.
- Taxon
- Medicago sativaMedicago sativae1
- Organ / stage
- differentiation zonee0
- Treatment
- 45 days at temperature of 10°Ce0
- Outcome
- 2C:4Ce0
- Value
- 70:30e0
- Comparator
- control temperaturee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root treated for 45 days at temperature of 10°C the 2C:4C in meristem apex is 78:22, elongation zone 74:26 and in differentiation zone is 70:30”
e1“Medicago sativa cv. Sitel”
e2“control temperature percentage of nuclei 2C:4C in meristem apex is 65:35, in elongation zone 50:50 and in differentiation zone is 40:60”
Claim · model-written summary
At 5°C, meristem apex 2C:4C proportion was 85:15.
- Taxon
- Medicago sativaMedicago sativae1
- Organ / stage
- meristem apexe0
- Treatment
- temperature of 5°Ce0
- Outcome
- 2C:4Ce2
- Value
- 85:15e0
- Comparator
- control temperaturee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“temperature of 5°C it was 85:15 in meristem apex, 80:20 in elongation zone and 71:29 in differentiation zone”
e1“Medicago sativa cv. Sitel”
e2“control temperature percentage of nuclei 2C:4C in meristem apex is 65:35”
Claim · model-written summary
At 5°C, elongation zone 2C:4C proportion was 80:20.
- Taxon
- Medicago sativaMedicago sativae1
- Organ / stage
- elongation zonee0
- Treatment
- temperature of 5°Ce0
- Outcome
- 2C:4Ce2
- Value
- 80:20e0
- Comparator
- control temperaturee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“temperature of 5°C it was 85:15 in meristem apex, 80:20 in elongation zone and 71:29 in differentiation zone”
e1“Medicago sativa cv. Sitel”
e2“control temperature percentage of nuclei 2C:4C in meristem apex is 65:35, in elongation zone 50:50”
Claim · model-written summary
At 5°C, differentiation zone 2C:4C proportion was 71:29.
- Taxon
- Medicago sativaMedicago sativae1
- Organ / stage
- differentiation zonee0
- Treatment
- temperature of 5°Ce0
- Outcome
- 2C:4Ce2
- Value
- 71:29e0
- Comparator
- control temperaturee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“temperature of 5°C it was 85:15 in meristem apex, 80:20 in elongation zone and 71:29 in differentiation zone”
e1“Medicago sativa cv. Sitel”
e2“control temperature percentage of nuclei 2C:4C in meristem apex is 65:35, in elongation zone 50:50 and in differentiation zone is 40:60”
Claim · model-written summary
GUS marker expression appeared in highly divided pre-treated roots at control temperature.
- Taxon
- Medicago falcata
Medicago sativa subsp. falcatae1 - Organ / stage
- roote0
- Treatment
- control temperaturee0
- Gene
- GUS genee2
- Outcome
- expression of marker genee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of marker gene was observed in highly divided cells of pre-treated root at control temperature”
e1“transgenic Medicago falcata plants”
e2“GUS gene”
Claim · model-written summary
GUS was not expressed in roots pre-treated at low temperature for 45 days.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae1
- Organ / stage
- rootse0
- Treatment
- low temperature for 45 dayse0
- Gene
- GUS genee0
- Outcome
- GUS gene was not expressede0
- Comparator
- control (24°C)e2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GUS gene was not expressed in pre-treated roots exposed to low temperature for 45 days”
e1“transgenic Medicago falcata plants”
e2“control (24°C) and low (5°C) temperatures”
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