Abstract
By interference of the yeast pheromone mitogen-activated protein kinase (MAPK) pathway with an alfalfa cDNA expression library, we have isolated the MP2C gene encoding a functional protein phosphatase type 2C. Epistasis analysis in yeast indicated that the molecular target of the MP2C phosphatase is Ste11, a MAPK kinase kinase that is a central regulator of the pheromone and osmosensing pathways. In plants, MP2C functions as a negative regulator of the stress-activated MAPK (SAMK) pathway that is activated by cold, drought, touch, and wounding. Although activation of the SAMK pathway occurs by a posttranslational mechanism, de novo transcription and translation of protein factor(s) are necessary for its inactivation. MP2C is likely to be this or one of these factors, because wound-induced activation of SAMK is followed by MP2C gene expression and recombinant glutathione S-transferase-MP2C is able to inactivate extracts containing wound-induced SAMK. Wound-induced MP2C expression is a transient event and correlates with the refractory period, i.e., the time when restimulation of the SAMK pathway is not possible by a second stimulation. These data suggest that MP2C is part of a negative feedback mechanism that is responsible for resetting the SAMK cascade in plants.
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Claims 9
Claim · model-written summary
An alfalfa cDNA screen isolated MP2C encoding a protein phosphatase type 2C.
- Taxon
- alfalfa
Medicago sativae0 - Gene
- MP2Ce0
- Outcome
- functional protein phosphatase type 2Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an alfalfa cDNA expression library, we have isolated the MP2C gene encoding a functional protein phosphatase type 2C”
Claim · model-written summary
Yeast epistasis implicated Ste11 as a molecular target of MP2C.
- Taxon
- alfalfaMedicago sativae1
- Gene
- MP2Ce0
- Outcome
- molecular target of the MP2C phosphatase is Ste11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Epistasis analysis in yeast indicated that the molecular target of the MP2C phosphatase is Ste11”
e1“alfalfa cDNA expression library”
Claim · model-written summary
MP2C negatively regulates the plant SAMK pathway.
- Taxon
- alfalfaMedicago sativae1
- Gene
- MP2Ce0
- Outcome
- stress-activated MAPK (SAMK) pathwaye0
- Direction
- negative regulatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In plants, MP2C functions as a negative regulator of the stress-activated MAPK (SAMK) pathway”
e1“alfalfa cDNA expression library”
Claim · model-written summary
SAMK was activated by cold, drought, touch and wounding.
- Taxon
- alfalfaMedicago sativae2
- Treatment
- cold, drought, touch, and woundinge0
- Gene
- SAMKe1
- Outcome
- stress-activated MAPK (SAMK) pathwaye1
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“activated by cold, drought, touch, and wounding”
e1“stress-activated MAPK (SAMK) pathway”
e2“alfalfa cDNA expression library”
Claim · model-written summary
SAMK inactivation required new transcription and translation of protein factors.
- Taxon
- alfalfaMedicago sativae2
- Gene
- SAMKe1
- Outcome
- inactivatione0
- Qualifier
- de novo transcription and translation of protein factor(s)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“de novo transcription and translation of protein factor(s) are necessary for its inactivation”
e1“stress-activated MAPK (SAMK) pathway”
e2“alfalfa cDNA expression library”
Claim · model-written summary
Wounding-induced SAMK activation was followed by MP2C gene expression.
- Taxon
- alfalfaMedicago sativae1
- Treatment
- wound-inducede0
- Gene
- MP2Ce0
- Outcome
- MP2C gene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wound-induced activation of SAMK is followed by MP2C gene expression”
e1“alfalfa cDNA expression library”
Claim · model-written summary
Recombinant GST-MP2C inactivated extracts containing wound-induced SAMK.
- Taxon
- alfalfaMedicago sativae1
- Treatment
- recombinant glutathione S-transferase-MP2Ce0
- Gene
- glutathione S-transferase-MP2Ce0
- Outcome
- wound-induced SAMKe0
- Direction
- inactivatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recombinant glutathione S-transferase-MP2C is able to inactivate extracts containing wound-induced SAMK”
e1“alfalfa cDNA expression library”
Claim · model-written summary
Wound-induced MP2C expression was transient and correlated with the SAMK refractory period.
- Taxon
- alfalfaMedicago sativae1
- Treatment
- Wound-inducede0
- Gene
- MP2Ce0
- Outcome
- refractory periode0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wound-induced MP2C expression is a transient event and correlates with the refractory period”
e1“alfalfa cDNA expression library”
Claim · model-written summary
Authors suggest MP2C participates in negative feedback that resets plant SAMK signaling.
- Taxon
- alfalfaMedicago sativae1
- Gene
- MP2Ce0
- Outcome
- resetting the SAMK cascadee0
- Qualifier
- negative feedback mechanisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MP2C is part of a negative feedback mechanism that is responsible for resetting the SAMK cascade in plants”
e1“alfalfa cDNA expression library”
The same questions in other papers
mechanical · quality: protein · Medicago sativa (alfalfa)Positive and negative results
1 in the opposite direction
- no effect
drought · quality: protein · Medicago sativa (alfalfa)Positive and negative results
5 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
5 papers more in this group
See all 9 papers →cold · quality: protein · Medicago sativa (alfalfa)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.