Abstract
A cDNA encoding a dehydrin was isolated from the flavedo of the chilling-sensitive Fortune mandarin fruit (Citrus clementina Hort. Ex Tanaka x Citrus reticulata Blanco) and designed as Crcor15. The predicted CrCOR15 protein is a K2S member of a closely related dehydrin family from Citrus, since it contains two tandem repeats of the unusual Citrus K-segment and one S-segment (serine cluster) at an unusual C-terminal position. Crcor15 mRNA is consistently and highly expressed in the flavedo during fruit development and maturation. The relative abundance of Crcor15 mRNA in the flavedo was estimated to be higher than 1% of total RNA. The high mRNA level remained unchanged during fruit storage at chilling (2 degrees C) and nonchilling (12 degrees C) temperatures, and it was depressed by a conditioning treatment (3 days at 37 degrees C) that induced chilling tolerance. Therefore, the expression of Crcor15 appears not to be related to the acquisition of chilling tolerance in mandarin fruits. However, Crcor15, which was barely detected in unstressed mandarin leaves, was rapidly induced in response to both low temperature and water stress. COR15 protein was expressed in Escherichia coli, and the purified protein conferred in vitro protection against freezing and dehydration inactivation. The potential role of Citrus COR15 is discussed.
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Claims 10
Claim · model-written summary
A cDNA encoding the dehydrin Crcor15 was isolated from Fortune mandarin flavedo.
Verbatim
e0“A cDNA encoding a dehydrin was isolated from the flavedo of the chilling-sensitive Fortune mandarin fruit (Citrus clementina Hort. Ex Tanaka x Citrus reticulata Blanco) and designed as Crcor15.”
Claim · model-written summary
CrCOR15 has two Citrus K-segment repeats and one C-terminal S-segment.
- Outcome
- two tandem repeats of the unusual Citrus K-segment and one S-segment (serine cluster)e0
CitrusNot reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it contains two tandem repeats of the unusual Citrus K-segment and one S-segment (serine cluster) at an unusual C-terminal position.”
t0“Dehydrin fromCitrus”
Claim · model-written summary
Crcor15 mRNA was consistently and highly expressed in flavedo during fruit development and maturation.
Verbatim
e0“Crcor15 mRNA is consistently and highly expressed in the flavedo during fruit development and maturation.”
t0“Fortune mandarin fruit”
Claim · model-written summary
Crcor15 mRNA in flavedo exceeded 1% of total RNA.
Verbatim
e0“The relative abundance of Crcor15 mRNA in the flavedo was estimated to be higher than 1% of total RNA.”
t0“Fortune mandarin fruit”
Claim · model-written summary
Crcor15 mRNA remained unchanged during chilling and nonchilling fruit storage.
- Taxon
- mandarint0
- Organ / stage
- fruite0
- Treatment
- fruit storage at chilling (2 degrees C) and nonchilling (12 degrees C) temperaturese0
- Gene
- Crcor15e1
- Outcome
- mRNA levele0
- Direction
- remained unchangede0
Not reportedComparator
Verbatim
e0“The high mRNA level remained unchanged during fruit storage at chilling (2 degrees C) and nonchilling (12 degrees C) temperatures”
t0“Fortune mandarin fruit”
e1“Crcor15 mRNA is consistently and highly expressed”
Claim · model-written summary
Conditioning that induced chilling tolerance depressed Crcor15 mRNA level.
- Taxon
- mandarint0
- Treatment
- conditioning treatment (3 days at 37 degrees C)e0
- Gene
- Crcor15e2
- Outcome
- mRNA levele1
- Direction
- depressede0
Not reportedComparator
Verbatim
e0“it was depressed by a conditioning treatment (3 days at 37 degrees C) that induced chilling tolerance.”
t0“Fortune mandarin fruit”
e1“The high mRNA level remained unchanged”
e2“Crcor15 mRNA is consistently and highly expressed”
Claim · model-written summary
Crcor15 was rapidly induced in leaves by low temperature.
Verbatim
e0“Crcor15, which was barely detected in unstressed mandarin leaves, was rapidly induced in response to both low temperature and water stress.”
Claim · model-written summary
Crcor15 was rapidly induced in leaves by water stress.
Verbatim
e0“Crcor15, which was barely detected in unstressed mandarin leaves, was rapidly induced in response to both low temperature and water stress.”
Claim · model-written summary
Purified COR15 protein protected against freezing inactivation in vitro.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the purified protein conferred in vitro protection against freezing and dehydration inactivation.”
t0“Citrus”
e1“COR15 protein was expressed in Escherichia coli”
Claim · model-written summary
Purified COR15 protein protected against dehydration inactivation in vitro.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the purified protein conferred in vitro protection against freezing and dehydration inactivation.”
t0“Citrus”
e1“COR15 protein was expressed in Escherichia coli”
The same questions in other papers
cold · molecular: expression · Citrus reticulata (tangerine)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.