Abstract
Polyphenol oxidase (PPO) activity of Russet Burbank potato was inhibited by sense and antisense PPO RNAs expressed from a tomato PPO cDNA under the control of the 35S promoter from the cauliflower mosaic virus. Transgenic Russet Burbank potato plants from 37 different lines were grown in the field. PPO activity and the level of enzymatic browning were measured in the tubers harvested from the field. Of the tubers from 28 transgenic lines that were sampled, tubers from 5 lines exhibited reduced browning. The level of PPO activity correlated with the reduction in enzymatic browning in these lines. These results indicate that expression of tomato PPO RNA in sense or antisense orientation inhibits PPO activity and enzymatic browning in the major commercial potato cultivar. Expression of tomato PPO RNA in sense orientation led to the greatest decrease in PPO activity and enzymatic browning, possibly due to cosuppression. These results suggest that expression of closely related heterologous genes can be used to prevent enzymatic browning in a wide variety of food crops without the application of various food additives.
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Claims 5
Claim · model-written summary
Tubers from 5 of 28 transgenic potato lines exhibited reduced browning.
- Taxon
- potato
Solanum tuberosume1 - Material
- transgenic linese2
- Outcome
- reduced browninge2
- Direction
- reducede2
- Value
- 5 linese2
- Setting
- fielde1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transgenic Russet Burbank potato plants from 37 different lines were grown in the field”
e2“Of the tubers from 28 transgenic lines that were sampled, tubers from 5 lines exhibited reduced browning”
Claim · model-written summary
The level of PPO activity correlated with the reduction in enzymatic browning in these potato lines.
- Taxon
- potatoSolanum tuberosume1
- Gene
- PPOe3
- Outcome
- reduction in enzymatic browninge3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transgenic Russet Burbank potato plants from 37 different lines were grown in the field”
e3“The level of PPO activity correlated with the reduction in enzymatic browning in these lines”
Claim · model-written summary
Expression of tomato PPO RNA in sense or antisense orientation inhibited PPO activity and enzymatic browning in the potato cultivar.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- expression of tomato PPO RNA in sense or antisense orientatione4
- Outcome
- PPO activity and enzymatic browninge4
- Direction
- inhibitse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transgenic Russet Burbank potato plants from 37 different lines were grown in the field”
e4“expression of tomato PPO RNA in sense or antisense orientation inhibits PPO activity and enzymatic browning in the major commercial potato cultivar”
Claim · model-written summary
Expression of tomato PPO RNA in sense orientation gave the greatest decrease in PPO activity and enzymatic browning compared with antisense orientation, possibly due to cosuppression.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- Expression of tomato PPO RNA in sense orientatione5
- Outcome
- PPO activity and enzymatic browninge5
- Direction
- greatest decreasee5
- Qualifier
- possibly due to cosuppressione5
- Comparator
- antisense orientatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transgenic Russet Burbank potato plants from 37 different lines were grown in the field”
e4“expression of tomato PPO RNA in sense or antisense orientation inhibits PPO activity and enzymatic browning in the major commercial potato cultivar”
e5“Expression of tomato PPO RNA in sense orientation led to the greatest decrease in PPO activity and enzymatic browning, possibly due to cosuppression”
Claim · model-written summary
Expression of closely related heterologous genes can be used to prevent enzymatic browning in a wide variety of food crops.
Verbatim
e6“expression of closely related heterologous genes can be used to prevent enzymatic browning in a wide variety of food crops”
The same questions in other papers
polyphenol · physiology: antioxidant enzymes · Solanum tuberosum (potato)Results agree
2 papers in the same direction as this paper
- positive
- positive
loss of function · physiology: antioxidant enzymes · Solanum tuberosum (potato)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.