Abstract
We have isolated 20 cDNA clones for which the corresponding mRNAs accumulate 2- to 20-fold within 4 hr of cytokinin addition to cytokinin-starved soybean suspension cultures. These changes in mRNA levels precede the greening and cell growth induced by cytokinin in this system. Treatment with cycloheximide before cytokinin addition enhances the accumulation of these messages. In addition, the abundance of these mRNAs is influenced by auxin. We demonstrate that the mRNAs corresponding to the cDNAs in this collection display a variety of patterns of accumulation in the tissues of an intact plant. We also show that different cytokinins induce similar increases in message levels. However, analogs of cytokinin that are much less active in bioassays induce lower levels of message accumulation. Sequence analyses indicate that two of the cDNAs correspond to ribosomal protein genes. The remaining cDNAs are likely to represent previously undiscovered genes that function early in the initiation of plant cell growth and/or chloroplast development.
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Claims 9
Claim · model-written summary
Twenty cDNA clones corresponded to mRNAs that accumulated 2- to 20-fold within four hours of cytokinin addition.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- soybean suspension culturese0
- Treatment
- cytokinin additione0
- Outcome
- corresponding mRNAs accumulatee0
- Value
- 2- to 20-folde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have isolated 20 cDNA clones for which the corresponding mRNAs accumulate 2- to 20-fold within 4 hr of cytokinin addition to cytokinin-starved soybean suspension cultures.”
t0“soybean”
Claim · model-written summary
Cytokinin-induced mRNA accumulation preceded greening and cell growth.
- Taxon
- soybeanGlycine maxt0
- Treatment
- cytokinine0
- Outcome
- changes in mRNA levels precede the greening and cell growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These changes in mRNA levels precede the greening and cell growth induced by cytokinin in this system.”
t0“soybean”
Claim · model-written summary
Cycloheximide pretreatment enhanced cytokinin-induced message accumulation.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Treatment with cycloheximide before cytokinin additione0
- Outcome
- accumulation of these messagese0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with cycloheximide before cytokinin addition enhances the accumulation of these messages.”
t0“soybean”
Claim · model-written summary
Auxin influenced abundance of the cytokinin-induced mRNAs.
- Taxon
- soybeanGlycine maxt0
- Treatment
- auxine0
- Outcome
- abundance of these mRNAse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the abundance of these mRNAs is influenced by auxin.”
t0“soybean”
Claim · model-written summary
The mRNAs showed varied accumulation patterns across tissues of intact plants.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- tissues of an intact plante0
- Outcome
- variety of patterns of accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mRNAs corresponding to the cDNAs in this collection display a variety of patterns of accumulation in the tissues of an intact plant.”
t0“soybean”
Claim · model-written summary
Different cytokinins induced similar increases in message levels.
- Taxon
- soybeanGlycine maxt0
- Treatment
- different cytokininse0
- Outcome
- message levelse0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“different cytokinins induce similar increases in message levels.”
t0“soybean”
Claim · model-written summary
Less active cytokinin analogs induced lower message accumulation.
- Taxon
- soybeanGlycine maxt0
- Treatment
- analogs of cytokinine0
- Outcome
- levels of message accumulatione0
- Direction
- lowere0
- Qualifier
- much less active in bioassayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“analogs of cytokinin that are much less active in bioassays induce lower levels of message accumulation.”
t0“soybean”
Claim · model-written summary
Two cDNAs corresponded to ribosomal protein genes.
- Taxon
- soybeanGlycine maxt0
- Gene
- ribosomal protein genese0
- Outcome
- two of the cDNAs correspond to ribosomal protein genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequence analyses indicate that two of the cDNAs correspond to ribosomal protein genes.”
t0“soybean”
Claim · model-written summary
The remaining cDNAs likely represent previously undiscovered genes involved early in cell growth or chloroplast development.
- Taxon
- soybeanGlycine maxt0
- Gene
- previously undiscovered genese0
- Outcome
- initiation of plant cell growth and/or chloroplast developmente0
- Qualifier
- likelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The remaining cDNAs are likely to represent previously undiscovered genes that function early in the initiation of plant cell growth and/or chloroplast development.”
t0“soybean”
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.