Abstract
Ethylene-forming enzyme (EFE) was isolated from apple (Malus domestica Borkh. cv Golden Delicious) fruit tissue. The enzyme activity in the homogenate is associated with the pellet fraction and can be solubilized with Triton X-100 or polyvinylpolypyrrolidone. The solubilized enzyme system resembles the in vivo system in that it exhibits a low K(m) (17 micromolar) for its substrate 1-aminocyclopropane-1-carboxylic acid (ACC), is stereospecific toward 2-ethyl-ACC stereoisomers for 1-butene production, and is inhibited by cobalt ions and alpha-aminoisobutyric acid. Intact preclimacteric fruits treated with exogenous ethylene showed a marked increase in in vivo EFE activity and this increase was accompanied by a parallel increase in in vitro EFE activity. These results support the notion that the isolated EFE represents the authentic in vivo activity.
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Claims 9
Claim · model-written summary
Ethylene-forming enzyme activity in apple homogenate was associated with the pellet fraction.
- Taxon
- apple
Malus domesticat0 - Organ / stage
- fruitt0
- Gene
- Ethylene-forming enzymet0
- Outcome
- enzyme activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme activity in the homogenate is associated with the pellet fraction”
t0“Ethylene-Forming Enzyme from Apple Fruit”
Claim · model-written summary
Triton X-100 solubilized the apple ethylene-forming enzyme.
- Taxon
- appleMalus domesticat0
- Treatment
- Triton X-100e0
- Gene
- Ethylene-forming enzymet0
- Outcome
- solubilizede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“can be solubilized with Triton X-100 or polyvinylpolypyrrolidone.”
t0“Ethylene-Forming Enzyme from Apple Fruit”
Claim · model-written summary
Polyvinylpolypyrrolidone solubilized the apple ethylene-forming enzyme.
- Taxon
- appleMalus domesticat0
- Treatment
- polyvinylpolypyrrolidonee0
- Gene
- Ethylene-forming enzymet0
- Outcome
- solubilizede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“can be solubilized with Triton X-100 or polyvinylpolypyrrolidone.”
t0“Ethylene-Forming Enzyme from Apple Fruit”
Claim · model-written summary
The solubilized enzyme had a low K(m) of 17 micromolar for ACC.
- Taxon
- appleMalus domesticat0
- Gene
- Ethylene-forming enzymet0
- Compound
- 1-aminocyclopropane-1-carboxylic acide0
- Outcome
- K(m)e0
- Direction
- lowe0
- Value
- 17 micromolare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it exhibits a low K(m) (17 micromolar) for its substrate 1-aminocyclopropane-1-carboxylic acid (ACC)”
t0“Ethylene-Forming Enzyme from Apple Fruit”
Claim · model-written summary
The solubilized enzyme was stereospecific toward 2-ethyl-ACC stereoisomers for 1-butene production.
- Taxon
- appleMalus domesticat0
- Gene
- Ethylene-forming enzymet0
- Outcome
- stereospecific toward 2-ethyl-ACC stereoisomers for 1-butene productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is stereospecific toward 2-ethyl-ACC stereoisomers for 1-butene production”
t0“Ethylene-Forming Enzyme from Apple Fruit”
Claim · model-written summary
Cobalt ions inhibited solubilized apple ethylene-forming enzyme.
- Taxon
- appleMalus domesticat0
- Treatment
- cobalt ionse0
- Gene
- Ethylene-forming enzymet0
- Outcome
- inhibitede0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is inhibited by cobalt ions and alpha-aminoisobutyric acid.”
t0“Ethylene-Forming Enzyme from Apple Fruit”
Claim · model-written summary
Alpha-aminoisobutyric acid inhibited solubilized apple ethylene-forming enzyme.
- Taxon
- appleMalus domesticat0
- Treatment
- alpha-aminoisobutyric acide0
- Gene
- Ethylene-forming enzymet0
- Outcome
- inhibitede0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is inhibited by cobalt ions and alpha-aminoisobutyric acid.”
t0“Ethylene-Forming Enzyme from Apple Fruit”
Claim · model-written summary
Exogenous ethylene markedly increased in vivo EFE activity in preclimacteric apple fruit.
- Taxon
- appleMalus domesticat0
- Organ / stage
- preclimacteric fruitse0
- Treatment
- exogenous ethylenee0
- Gene
- EFEe0
- Outcome
- in vivo EFE activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Intact preclimacteric fruits treated with exogenous ethylene showed a marked increase in in vivo EFE activity”
t0“Ethylene-Forming Enzyme from Apple Fruit”
Claim · model-written summary
Exogenous ethylene increased in vitro EFE activity in parallel with the in vivo increase.
- Taxon
- appleMalus domesticat0
- Treatment
- exogenous ethylenee0
- Gene
- EFEe0
- Outcome
- in vitro EFE activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Intact preclimacteric fruits treated with exogenous ethylene showed a marked increase in in vivo EFE activity and this increase was accompanied by a parallel increase in in vitro EFE activity.”
t0“Ethylene-Forming Enzyme from Apple Fruit”
The same questions in other papers
ethene · physiology: hormones · Malus domestica (apple)Results differ
4 papers in the same direction as this paper · 6 in the opposite direction
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.