Abstract
In plants gibberellins (GAs) are responsible for triggering stem or internodal elongation. To comprehend the molecular basis of internodal elongation in rice, a proteomics approach using differentially displayed proteins on two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was carried out to identify the proteins expressed during the GA controlled leaf-sheath elongation response. Out of 352 protein spots detected on 2-D PAGE, 32 proteins showed modulation in the expression levels in GA3-treated leaf-sheath for 48 h as compared to control. These proteins were analyzed using protein sequencer and/or mass spectrometry in conjunction with the protein database to assign putative identities. The twin protein spots (LS079 and LS083), identified as calreticulin, showed different isoelectric points and expression level in GA3-treated leaf-sheath. The expression level of LS083 (pI 4.0) was down-regulated as compared to the up-regulation of LS079 (pI 4.3). In the presence of GA3 and growth inhibitor, uniconazole and abscisic acid, respectively, no elongation in leaf-sheath occurred and calreticulin did not shift from LS083 to LS079. Over-expression of calreticulin in rice inhibited the callus regeneration and seedling growth. These results suggest that calreticulin is an important component in the GA signaling pathway that regulates rice seedling leaf-sheath elongation.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
After 48 h of GA3, 32 of 352 protein spots changed expression relative to control.
- Taxon
- Rice (Oryza sativa L.)
Oryza sativat0 - Organ / stage
- leaf-sheathe0
- Treatment
- GA3-treatede0
- Outcome
- modulation in the expression levelse0
- Value
- 32 proteinse0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Out of 352 protein spots detected on 2-D PAGE, 32 proteins showed modulation in the expression levels in GA3-treated leaf-sheath for 48 h as compared to control.”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
Calreticulin spots LS079 and LS083 differed in isoelectric point and expression under GA3.
- Taxon
- Rice (Oryza sativa L.)Oryza sativat0
- Organ / stage
- leaf-sheathe0
- Treatment
- GA3-treatede0
- Gene
- calreticuline0
- Outcome
- different isoelectric points and expression levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The twin protein spots (LS079 and LS083), identified as calreticulin, showed different isoelectric points and expression level in GA3-treated leaf-sheath.”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
LS083 expression was down-regulated under GA3.
- Taxon
- Rice (Oryza sativa L.)Oryza sativat0
- Gene
- LS083e0
- Outcome
- expression level of LS083e0
- Direction
- down-regulatede0
- Value
- pI 4.0e0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression level of LS083 (pI 4.0) was down-regulated”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
LS079 expression was up-regulated relative to LS083.
- Taxon
- Rice (Oryza sativa L.)Oryza sativat0
- Gene
- LS079e0
- Outcome
- LS079e0
- Direction
- up-regulatione0
- Value
- pI 4.3e0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the up-regulation of LS079 (pI 4.3)”
e1“as compared to the up-regulation of LS079 (pI 4.3)”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
With GA3 and growth inhibitors, leaf-sheath elongation did not occur.
- Taxon
- Rice (Oryza sativa L.)Oryza sativat0
- Organ / stage
- leaf-sheathe0
- Treatment
- GA3 and growth inhibitor, uniconazole and abscisic acide0
- Outcome
- no elongation in leaf-sheathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the presence of GA3 and growth inhibitor, uniconazole and abscisic acid, respectively, no elongation in leaf-sheath occurred”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
With GA3 and growth inhibitors, calreticulin did not shift from LS083 to LS079.
- Taxon
- Rice (Oryza sativa L.)Oryza sativat0
- Treatment
- GA3 and growth inhibitor, uniconazole and abscisic acide1
- Gene
- calreticuline0
- Outcome
- calreticulin did not shift from LS083 to LS079e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“calreticulin did not shift from LS083 to LS079”
e1“In the presence of GA3 and growth inhibitor, uniconazole and abscisic acid, respectively”
t0“Rice (Oryza sativa L.)”
Claim · model-written summary
Calreticulin over-expression inhibited rice callus regeneration.
- Taxon
- riceOryza sativae0
- Treatment
- Over-expression of calreticuline0
- Gene
- calreticuline0
- Outcome
- callus regeneratione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Over-expression of calreticulin in rice inhibited the callus regeneration and seedling growth.”
Claim · model-written summary
Calreticulin over-expression inhibited rice seedling growth.
- Taxon
- riceOryza sativae0
- Treatment
- Over-expression of calreticuline0
- Gene
- calreticuline0
- Outcome
- seedling growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Over-expression of calreticulin in rice inhibited the callus regeneration and seedling growth.”
Claim · model-written summary
The authors suggest calreticulin participates in GA signaling regulating sheath elongation.
- Taxon
- riceOryza sativae0
- Gene
- calreticuline0
- Outcome
- seedling leaf-sheath elongatione0
- Qualifier
- GA signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“calreticulin is an important component in the GA signaling pathway that regulates rice seedling leaf-sheath elongation”
The same questions in other papers
Gibberellic acid · growth · Oryza sativa (rice)Results differ
17 papers in the same direction as this paper · 86 in the opposite direction
- positive
- positive
- positive
100 papers more in this group
See all 104 papers →uniconazole · growth · Oryza sativa (rice)Positive and negative results
6 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.