Abstract
Plants possess two types of phosphofructokinase proteins for phosphorylation of fructose-6-phosphate, the ATP-dependent phosphofructokinase (PFK) and the pyrophosphate-(PPi) dependent pyrophosphate-fructose-6-phosphate-phosphotransferase (PFP). During oxygen deficiency ATP levels in rice seedlings are severely reduced, and it is hypothesized that PPi is used as an alternative energy source for the phosphorylation of fructose-6-phosphate during glycolysis. In this study, we analyzed the expression of 15 phosphofructokinase-encoding genes in roots and aerial tissues of anoxia-tolerant rice seedlings in response to anoxic stress and compared our data with transcript profiles obtained from microarray analyses. Furthermore, the intracellular localization of rice PFK proteins was determined, and the PFK and PFP isoforms were grouped in a phylogenetic tree. Two PFK and two PFP transcripts accumulated during anoxic stress, whereas mRNA levels of four PFK and three PFP genes were decreased. The total specific activity of both PFK and PFP changed only slightly during a 24-h anoxia treatment. It is assumed that expression of different isoforms and their catalytic properties differ during normoxic and anoxic conditions and contribute to balanced glycolytic activity during the low-oxygen stress. These characterizations of phosphofructokinase genes and the comparison to other plant species allowed us to suggest candidate rice genes for adaptation to anoxic stress.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Two PFK transcripts accumulated during anoxic stress in rice seedlings.
- Taxon
- rice
Oryza sativae1 - Organ / stage
- roots and aerial tissuese1
- Treatment
- anoxic stresse2
- Gene
- PFKe2
- Outcome
- transcriptse2
- Direction
- accumulatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we analyzed the expression of 15 phosphofructokinase-encoding genes in roots and aerial tissues of anoxia-tolerant rice seedlings in response to anoxic stress”
e2“Two PFK and two PFP transcripts accumulated during anoxic stress”
Claim · model-written summary
Two PFP transcripts accumulated during anoxic stress in rice seedlings.
- Taxon
- riceOryza sativae1
- Organ / stage
- roots and aerial tissuese1
- Treatment
- anoxic stresse2
- Gene
- PFPe2
- Outcome
- transcriptse2
- Direction
- accumulatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we analyzed the expression of 15 phosphofructokinase-encoding genes in roots and aerial tissues of anoxia-tolerant rice seedlings in response to anoxic stress”
e2“Two PFK and two PFP transcripts accumulated during anoxic stress”
Claim · model-written summary
mRNA levels of four PFK genes decreased under anoxic stress in rice seedlings.
- Taxon
- riceOryza sativae1
- Organ / stage
- roots and aerial tissuese1
- Treatment
- anoxic stresse1
- Gene
- PFKe3
- Outcome
- mRNA levelse3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we analyzed the expression of 15 phosphofructokinase-encoding genes in roots and aerial tissues of anoxia-tolerant rice seedlings in response to anoxic stress”
e3“whereas mRNA levels of four PFK and three PFP genes were decreased”
Claim · model-written summary
mRNA levels of three PFP genes decreased under anoxic stress in rice seedlings.
- Taxon
- riceOryza sativae1
- Organ / stage
- roots and aerial tissuese1
- Treatment
- anoxic stresse1
- Gene
- PFPe3
- Outcome
- mRNA levelse3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we analyzed the expression of 15 phosphofructokinase-encoding genes in roots and aerial tissues of anoxia-tolerant rice seedlings in response to anoxic stress”
e3“whereas mRNA levels of four PFK and three PFP genes were decreased”
Claim · model-written summary
Total specific activity of PFK and PFP changed only slightly during a 24-h anoxia treatment.
- Taxon
- riceOryza sativae1
- Treatment
- 24-h anoxia treatmente4
- Outcome
- total specific activity of both PFK and PFPe4
- Direction
- changed only slightlye4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we analyzed the expression of 15 phosphofructokinase-encoding genes in roots and aerial tissues of anoxia-tolerant rice seedlings in response to anoxic stress”
e4“The total specific activity of both PFK and PFP changed only slightly during a 24-h anoxia treatment”
Claim · model-written summary
Differential expression of PFK and PFP isoforms may contribute to balanced glycolytic activity during low-oxygen stress (assumed).
- Taxon
- riceOryza sativae1
- Outcome
- balanced glycolytic activitye5
- Qualifier
- expression of different isoforms and their catalytic properties differ during normoxic and anoxic conditionse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we analyzed the expression of 15 phosphofructokinase-encoding genes in roots and aerial tissues of anoxia-tolerant rice seedlings in response to anoxic stress”
e5“It is assumed that expression of different isoforms and their catalytic properties differ during normoxic and anoxic conditions and contribute to balanced glycolytic activity during the low-oxygen stress”
The same questions in other papers
flooding · nutrient: efficiency · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.