Abstract
A fragment of the first exon of the proline dehydrogenase gene of arabidopsis ( Arabidopsis thaliana L.) in antisense orientation was transferred to maize ( Zea mays L.) genome by agrobacterial transformation in planta to elevate the content of free proline in maize cells. The presence of genetic structure carrying an antisense sequence of the fragment of the proline dehydrogenase gene (ASPG) from the genome of diploid maize seedlings was corroborated by PCR method. T-DNA insertions comprising ASPG were found in the genomes of 30 plants (1.2% of 2409 examined seedlings) belonging to the generation T 0. A reliable 4.6-fold increase in proline content was registered in the leaves of transformed maize plants.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 2
Claim · model-written summary
ASPG T-DNA insertions were detected in 30 T0 maize plants, 1.2% of 2409 seedlings.
- Organ / stage
- seedlingse0
- Gene
- ASPGe0
- Outcome
- T-DNA insertionse0
- Value
- 30 plants (1.2% of 2409 examined seedlings)e0
Zea maysNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“T-DNA insertions comprising ASPG were found in the genomes of 30 plants (1.2% of 2409 examined seedlings) belonging to the generation T 0.”
t0“maize”
Claim · model-written summary
Transformed maize leaves had a reliable 4.6-fold increase in proline content.
- Material
- transformed maize plantse0
- Organ / stage
- leavese0
- Treatment
- antisense orientationt0
- Outcome
- proline contente0
- Direction
- increasee0
- Value
- 4.6-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A reliable 4.6-fold increase in proline content was registered in the leaves of transformed maize plants.”
t0“antisense orientation”
The same questions in other papers
loss of function · physiology: osmolytes · Zea mays (maize)Positive and negative results
2 in the opposite direction
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.