Abstract
We investigated the effect of total irradiation dose and dose rate on flower color mutation and nuclear DNA content as an index of radiation damage in chrysanthemum. Chrysanthemum morifolium cv. ‘Taihei’ plants grown by in vitro culture were gamma-irradiated with a total dose of 15, 30 and 60 Gy at a rate of 0.5, 1, 2 and 5 Gy/h. Leaf explants cut from the irradiated plants were tissue cultured, and the regeneration rates and frequency of flower color mutation were investigated. Nuclear DNA content was measured by flow cytometric analysis. The regeneration rate decreased with increase in the total dose and dose rate of irradiation. Mutation frequency did not differ significantly among dose rates, indicating that mutation frequency was independent of dose rate and was dependent mainly on total dose. Comparison of the average of the nuclear DNA content with each treatment revealed that it was influenced by both dose rate and total dose, and that the reduction in nuclear DNA content was less at low dose rates, even when total doses were high. It appears that same mutation frequencies were obtained without large reduction in nuclear DNA content by 0.5 Gy/h, when compared with 2 Gy/h. Consequently, we conclude that gamma ray irradiations of high total doses at low dose rates efficiently induce mutations with less radiation damage in chrysanthemum.
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Claims 7
Claim · model-written summary
Regeneration rate decreased as irradiation dose and dose rate increased.
- Taxon
- chrysanthemum
.jpeg?width=60)
Chrysanthemumt0 - Treatment
- total dose and dose rate of irradiatione0
- Outcome
- regeneration ratee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e0“The regeneration rate decreased with increase in the total dose and dose rate of irradiation.”
t0“chrysanthemum”
Claim · model-written summary
Mutation frequency did not differ significantly among dose rates.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- dose ratese0
- Outcome
- Mutation frequencye0
- Direction
- did not differe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation frequency did not differ significantly among dose rates”
t0“chrysanthemum”
Claim · model-written summary
Mutation frequency depended mainly on total dose.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- total dosee0
- Outcome
- mutation frequencye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutation frequency was independent of dose rate and was dependent mainly on total dose”
t0“chrysanthemum”
Claim · model-written summary
Nuclear DNA content was influenced by both dose rate and total dose.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- dose rate and total dosee0
- Outcome
- nuclear DNA contente1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was influenced by both dose rate and total dose”
e1“reduction in nuclear DNA content was less”
t0“chrysanthemum”
Claim · model-written summary
Reduction in nuclear DNA content was less at low dose rates even with high total doses.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- low dose ratese0
- Outcome
- nuclear DNA contente0
- Direction
- lesse0
- Qualifier
- even when total doses were highe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the reduction in nuclear DNA content was less at low dose rates, even when total doses were high”
t0“chrysanthemum”
Claim · model-written summary
At 0.5 Gy/h, mutation frequencies appeared similar to 2 Gy/h with less loss of nuclear DNA content.
- Taxon
- chrysanthemumChrysanthemumt0
- Treatment
- 0.5 Gy/he0
- Outcome
- mutation frequenciese0
- Direction
- samee0
- Comparator
- 2 Gy/he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“same mutation frequencies were obtained without large reduction in nuclear DNA content by 0.5 Gy/h, when compared with 2 Gy/h”
t0“chrysanthemum”
Claim · model-written summary
Low dose rate irradiation at high total doses was recommended to induce mutations with less damage.
- Taxon
- chrysanthemumChrysanthemume0
- Treatment
- gamma ray irradiations of high total doses at low dose ratese0
- Outcome
- efficiently induce mutations with less radiation damagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gamma ray irradiations of high total doses at low dose rates efficiently induce mutations with less radiation damage in chrysanthemum”
The same questions in other papers
radiation · growth · ChrysanthemumPositive and negative results
9 papers in the same direction as this paper · 10 in the opposite direction
- positive
- negative
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See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.