Abstract
A tonoplast-enriched membrane fraction was obtained from roots of barley (Hordeum vulgare cv CM72) seedlings grown in half-strength nutrient solution with or without 100 mM NaCl. Proton transport by the tonoplast ATPase was measured using acridine orange, quinacrine or uptake of ({sup 14}C)-methylamine. The Vmax for proton transport was 2 to 4-fold higher for the ATPase from salt-grown compared to control roots. However, the rate of ATP hydrolysis was not significantly different for the two treatments. The percent inhibition of ATP hydrolysis by DCCD, DIDS and KNO{sub 3} was similar for the two treatments, as was the percent stimulation by Cl. The pH optimum for proton transport was more alkaline for the ATPase from salt-grown roots. No difference was observed between membranes from control and salt-grown roots when oxonol fluorescence was used to measure formation of a membrane potential by the ATPase. Immunoblots of SDS gels were reacted with the antibody to the 68-kD subunit from red beet to assess the relative amount of the 68-kD subunit of the tonoplast ATPase from control or salt-grown roots. The relative amount of the 16-kD DCCD-binding subunit was compared by binding of ({sup 14}C)-DCCD. The amounts of the two subunits were not significantlymore » different in membranes from control or salt-grown roots. The increase in rate of formation of the pH gradient was not accounted for by an increase in the amount of the ATPase.« less
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Claims 8
Claim · model-written summary
Salt-grown barley roots had a 2 to 4-fold higher maximum proton transport rate than controls.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- salt-growne0
- Gene
- ATPasee0
- Outcome
- Vmax for proton transporte0
- Direction
- highere0
- Value
- 2 to 4-folde0
- Comparator
- control rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The Vmax for proton transport was 2 to 4-fold higher for the ATPase from salt-grown compared to control roots.”
t0“A tonoplast-enriched membrane fraction was obtained from roots of barley (Hordeum vulgare cv CM72) seedlings grown in half-strength nutrient solution with or without 100 mM NaCl.”
Claim · model-written summary
ATP hydrolysis rate did not differ significantly between salt-grown and control roots.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- salt-growne0
- Outcome
- rate of ATP hydrolysise0
- Direction
- not significantly differente0
- Comparator
- control rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Vmax for proton transport was 2 to 4-fold higher for the ATPase from salt-grown compared to control roots. However, the rate of ATP hydrolysis was not significantly different for the two treatments.”
t0“A tonoplast-enriched membrane fraction was obtained from roots of barley (Hordeum vulgare cv CM72) seedlings grown in half-strength nutrient solution with or without 100 mM NaCl.”
Claim · model-written summary
The inhibition of ATP hydrolysis by DCCD, DIDS and KNO3 was similar in both treatments.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- DCCD, DIDS and KNO{sub 3}e0
- Outcome
- percent inhibition of ATP hydrolysise0
- Direction
- similare0
- Comparator
- two treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percent inhibition of ATP hydrolysis by DCCD, DIDS and KNO{sub 3} was similar for the two treatments”
t0“A tonoplast-enriched membrane fraction was obtained from roots of barley (Hordeum vulgare cv CM72) seedlings grown in half-strength nutrient solution with or without 100 mM NaCl.”
Claim · model-written summary
Chloride stimulation of ATP hydrolysis was similar in both treatments.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- Cle0
- Outcome
- percent stimulation by Cle0
- Direction
- similare0
- Comparator
- two treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percent inhibition of ATP hydrolysis by DCCD, DIDS and KNO{sub 3} was similar for the two treatments, as was the percent stimulation by Cl.”
t0“A tonoplast-enriched membrane fraction was obtained from roots of barley (Hordeum vulgare cv CM72) seedlings grown in half-strength nutrient solution with or without 100 mM NaCl.”
Claim · model-written summary
The pH optimum for proton transport shifted to a more alkaline value in salt-grown roots.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- salt-growne0
- Outcome
- pH optimum for proton transporte0
- Direction
- more alkalinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pH optimum for proton transport was more alkaline for the ATPase from salt-grown roots.”
t0“A tonoplast-enriched membrane fraction was obtained from roots of barley (Hordeum vulgare cv CM72) seedlings grown in half-strength nutrient solution with or without 100 mM NaCl.”
Claim · model-written summary
Membrane potential formation did not differ between control and salt-grown roots.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- salt-grown rootse0
- Outcome
- formation of a membrane potentiale0
- Direction
- No differencee0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No difference was observed between membranes from control and salt-grown roots when oxonol fluorescence was used to measure formation of a membrane potential by the ATPase.”
t0“A tonoplast-enriched membrane fraction was obtained from roots of barley (Hordeum vulgare cv CM72) seedlings grown in half-strength nutrient solution with or without 100 mM NaCl.”
Claim · model-written summary
The 68-kD and 16-kD ATPase subunit amounts did not differ significantly between salt-grown and control roots.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- salt-grown rootse0
- Outcome
- amounts of the two subunitse0
- Direction
- not significantlymore » differente0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amounts of the two subunits were not significantlymore » different in membranes from control or salt-grown roots.”
t0“A tonoplast-enriched membrane fraction was obtained from roots of barley (Hordeum vulgare cv CM72) seedlings grown in half-strength nutrient solution with or without 100 mM NaCl.”
Claim · model-written summary
The faster pH gradient formation was not explained by more ATPase.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- increase in rate of formation of the pH gradiente0
- Qualifier
- not accounted for by an increase in the amount of the ATPasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase in rate of formation of the pH gradient was not accounted for by an increase in the amount of the ATPase.”
t0“A tonoplast-enriched membrane fraction was obtained from roots of barley (Hordeum vulgare cv CM72) seedlings grown in half-strength nutrient solution with or without 100 mM NaCl.”
The same questions in other papers
salinity · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 20 in the opposite direction
- no effect
- negative
- no effect
19 papers more in this group
See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.