Paper · 1978 · PLANT PHYSIOLOGY

Within-day Changes in the Polyribosome Content and in Synthesis of Proteins in Leaves of Capsicum annuum L

Barrie T. Steer; Graeme C. Blackwood

5
claims
9
verbatim quotes
6
citations
1
research areas
✓
Open access

Abstract

Capsicum annuum cv. California Wonder was grown in controlled environment with a 12-hour photoperiod. The polyribosome content of leaves varied from 60 to 72% of total ribosomes with the highest level occurring in the middle of the photoperiod and the lowest in the middle of the dark period. The variation was accounted for by changes in the content of large polyribosomes (hexamers and larger). There was no indication of an immediate effect on polyribosome content of light-on or light-off.The synthesis of proteins at two times in the 24-hour cycle was compared using a dual isotope technique. Statistically significant results were obtained that suggested that protein(s) with molecular weights of 26,000 daltons were preferentially synthesized in the photoperiod compared to the dark period. No evidence was found for the differential synthesis of proteins within the photoperiod.

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Claims 5

Observationobservedoriginal resultmeasured

Claim · model-written summary

Leaf polyribosomes ranged from 60 to 72% of total ribosomes, peaking mid-photoperiod and reaching a minimum mid-dark period.

Taxon
Capsicum annuumCapsicum annuume0
Material
California Wondere0
Organ / stage
leavese0
Outcome
polyribosome content of leavese0
Value
60 to 72%e0
Qualifier
highest level occurring in the middle of the photoperiod and the lowest in the middle of the dark periode0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Capsicum annuum cv. California Wonder was grown in controlled environment with a 12-hour photoperiod. The polyribosome content of leaves varied from 60 to 72% of total ribosomes with the highest level occurring in the middle of the photoperiod and the lowest in the middle of the dark period.”

✓ located verbatim in the source · abstract, characters 0–292 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Daily variation in polyribosomes was accounted for by changes in large polyribosome content.

Taxon
Capsicum annuumCapsicum annuumt0
Outcome
variatione0
Qualifier
changes in the content of large polyribosomes (hexamers and larger)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The variation was accounted for by changes in the content of large polyribosomes (hexamers and larger).”

✓ located verbatim in the source · abstract, characters 293–396 · show in text ↑
t0

“Capsicum annuum L”

✓ located verbatim in the source · title, characters 89–106 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Light-on or light-off had no indicated immediate effect on polyribosome content.

Taxon
Capsicum annuumCapsicum annuumt0
Treatment
light-on or light-offe0
Outcome
polyribosome contente0
Direction
no indication of an immediate effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“There was no indication of an immediate effect on polyribosome content of light-on or light-off.”

✓ located verbatim in the source · abstract, characters 397–493 · show in text ↑
t0

“Capsicum annuum L”

✓ located verbatim in the source · title, characters 89–106 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Proteins of molecular weight 26,000 daltons were preferentially synthesized during the photoperiod versus the dark period.

Taxon
Capsicum annuumCapsicum annuumt0
Outcome
protein(s) with molecular weights of 26,000 daltonse0
Direction
preferentially synthesizede0
Comparator
dark periode0
Setting
photoperiode0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Statistically significant results were obtained that suggested that protein(s) with molecular weights of 26,000 daltons were preferentially synthesized in the photoperiod compared to the dark period.”

✓ located verbatim in the source · abstract, characters 598–797 · show in text ↑
t0

“Capsicum annuum L”

✓ located verbatim in the source · title, characters 89–106 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

No differential protein synthesis was detected within the photoperiod.

Taxon
Capsicum annuumCapsicum annuumt0
Outcome
differential synthesis of proteinse0
Qualifier
within the photoperiode0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“No evidence was found for the differential synthesis of proteins within the photoperiod.”

✓ located verbatim in the source · abstract, characters 798–886 · show in text ↑
t0

“Capsicum annuum L”

✓ located verbatim in the source · title, characters 89–106 · show in text ↑

light · molecular: metabolites · Capsicum annuum (sweet pepper)Positive and negative results

7 papers: 4 positive, 3 negative or no effect

2 papers in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.