Abstract
Low‐temperature fluorescence emission spectra of epicotyls of 6.5‐day‐old dark‐grown seedlings of pea ( Pisum sativum L.) showed the dominance of short‐wavelength protoch lorophyllide forms with emission maxima at 629 and 636 nm, respectively. The presence of long‐wavelength protochlorophyllide with emission maxima around 650 nm was just detectable. Accordingly, irradiation with millisecond flashes gave a minute formation of chlorophyllide. The chlorophyll(ide) formation varied along the epicotyl. Irradiation with continuous light for 1.5 h resulted in an evident accumulation of chlorophyll(ide) in the upper part of the epicotyl. Only small amounts accumulated in the middle section. The conversion of protochlorophyllide to chlorophyllide was temperature dependent and almost arrested at 0°C. The chlorophyll(ide) formed had one dominating fluorescence peak at 681 nm. Irradiation for 24 h gave almost 100 times more chlorophyll in the upper part of the epicotyl than in the lower part. Electron micrographs from the upper part of the epicotyl irradiated for 6 h showed plastids with several developing thylakoids, while the plastids in the lower part of the epicotyl had only a few thylakoids. The dominance of short‐wavelength protochlorophyllide forms indicated the presence of protochlorophyllide not bound to the active site of NADPH‐protochlorophyllide oxidoreductase (EC 1.3.1.33). The inability of the short‐wavelength form to transform into chlorophyllide with flash light denotes a dislocation from the active site. The time and temperature dependence of the chlorophyll(ide) formation in continuous light indicates that a relocation is required of the short‐wavelength protochlorophyllide before chlorophyllide formation can occur.
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Claims 11
Claim · model-written summary
Short-wavelength protochlorophyllide forms dominated in dark-grown pea epicotyls, with emission maxima at 629 and 636 nm
- Taxon
- Pisum sativum
Lathyrus oleraceuse0 - Outcome
- dominancee1
- Value
- 629 and 636 nme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“showed the dominance of short‐wavelength protoch lorophyllide forms with emission maxima at 629 and 636 nm”
Claim · model-written summary
Long-wavelength protochlorophyllide was just detectable, with emission maxima around 650 nm
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Outcome
- presencee1
- Value
- around 650 nme1
- Qualifier
- was just detectablee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“The presence of long‐wavelength protochlorophyllide with emission maxima around 650 nm was just detectable”
Claim · model-written summary
Millisecond flash irradiation gave only a minute formation of chlorophyllide
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Treatment
- millisecond flashese1
- Outcome
- formation of chlorophyllidee1
- Direction
- minutee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“Irradiation with millisecond flashes gave a minute formation of chlorophyllide”
Claim · model-written summary
Continuous light for 1.5 h increased chlorophyll(ide) accumulation in the upper part of the epicotyl
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Organ / stage
- upper part of the epicotyle1
- Treatment
- continuous light for 1.5 he1
- Outcome
- accumulation of chlorophyll(ide)e1
- Direction
- increaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“Irradiation with continuous light for 1.5 h resulted in an evident accumulation of chlorophyll(ide) in the upper part of the epicotyl”
Claim · model-written summary
Only small amounts of chlorophyll(ide) accumulated in the middle section of the epicotyl
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Organ / stage
- middle sectione1
- Outcome
- accumulatede1
- Qualifier
- small amountse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“Only small amounts accumulated in the middle section”
Claim · model-written summary
Temperature strongly slowed the conversion of protochlorophyllide to chlorophyllide, which was almost arrested at 0°C
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Treatment
- temperaturee1
- Outcome
- conversion of protochlorophyllide to chlorophyllidee1
- Direction
- almost arrestede1
- Setting
- 0°Ce1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“The conversion of protochlorophyllide to chlorophyllide was temperature dependent and almost arrested at 0°C”
Claim · model-written summary
Irradiation for 24 h gave almost 100 times more chlorophyll in the upper part of the epicotyl than in the lower part
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Organ / stage
- upper part of the epicotyle1
- Treatment
- Irradiation for 24 he1
- Outcome
- chlorophylle1
- Direction
- moree1
- Value
- almost 100 timese1
- Comparator
- lower parte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“Irradiation for 24 h gave almost 100 times more chlorophyll in the upper part of the epicotyl than in the lower part”
Claim · model-written summary
Irradiation for 6 h gave plastids with several developing thylakoids in the upper epicotyl, versus only a few in the lower part
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Organ / stage
- plastidse1
- Treatment
- irradiated for 6 he1
- Outcome
- developing thylakoidse1
- Direction
- severale1
- Comparator
- lower parte3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“Electron micrographs from the upper part of the epicotyl irradiated for 6 h showed plastids with several developing thylakoids”
e2“had only a few thylakoids”
e3“Irradiation for 24 h gave almost 100 times more chlorophyll in the upper part of the epicotyl than in the lower part”
Claim · model-written summary
The dominance of short-wavelength forms indicates protochlorophyllide not bound to the active site of the reductase
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Outcome
- presence of protochlorophyllidee1
- Qualifier
- not bound to the active sitee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“indicated the presence of protochlorophyllide not bound to the active site”
Claim · model-written summary
The inability to transform into chlorophyllide with flash light denotes a dislocation of protochlorophyllide from the active site
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Outcome
- to transform into chlorophyllide with flash lighte1
- Qualifier
- denotes a dislocation from the active sitee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“to transform into chlorophyllide with flash light denotes a dislocation from the active site”
Claim · model-written summary
Relocation of short-wavelength protochlorophyllide is required before chlorophyllide formation can occur
- Taxon
- Pisum sativumLathyrus oleraceuse0
- Outcome
- chlorophyllide formatione2
- Qualifier
- a relocation is requirede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea ( Pisum sativum L.)”
e1“a relocation is required”
e2“before chlorophyllide formation can occur”
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.