Paper · 1996 · Phytopathology

Interactive Effects of Broccoli Residue and Temperature onVerticillium dahliaeMicrosclerotia in Soil and on Wilt in Cauliflower

K. Subbarao

15
claims
22
verbatim quotes
73
citations
2
research areas
—
Not open access

Abstract

The effects of dry, fresh, and no broccoli amendments on Verticillium dahliae microsclerotia in soil were evaluated at 10, 15, 20, 25, 30, and 35°C. Aliquots of 25 g of field soil naturally infested with V. dahliae microsclerotia (80 to 100 per gram of soil) were placed in plastic bottles to which dry (1% wvwt), fresh (8.7% wt/wt), or no broccoli treatments were randomly assigned. One randomly chosen set of each treatment was assigned to the above temperatures and incubated for 45 days. The soil was subsequently assayed for microsclerotia using the Anderson sampler technique. The experiment was repeated nine times. At all temperatures, both dry and fresh broccoli significantly (P < 0.05) reduced the number of microsclerotia in soil compared with the unamended soil. At ≤30°C, fresh broccoli had a significantly (P < 0.05) greater remissive effect than dry broccoli. At 35°C, however, the number of V. dahliae microsclerotia were significantly (P < 0.05) reduced after 45 days in unamended soil; both fresh and dry broccoli almost completely eliminated the pathogen from soil. Temporal dynamics of the V. dahliae microsclerotia as affected by the treatments at the above temperatures were determined in nine experiments identical to the above by sampling and assaying soil three times at 15-day intervals. Regardless of the temperature, maximum reductions in the number of microsclerotia in treatments involving broccoli occurred within 15 days, with fresh broccoli providing significantly (P < 0.05) greater reductions. Further reductions in the number of microsclerotia were not significant in broccoli treatments at the subsequent sampling dates. In unamended soil, the number of microsclerotia at all temperatures except 35°C changed little throughout the experiment. The optimum temperatures for the broccoli-mediated reductions in V. dahliae microsclerotia in both experiments were 25 and 30°C. Four greenhouse experiments were conducted to test the effectiveness of broccoli treatments in reducing wilt incidence in cauliflower. Consistently, cauliflower plants in the fresh broccoli treatment were taller, had greater root and shoot weights, were significantly more robust, and had the least number of infected plants (number of microsclerotia was lower) than in other treatments. The number of infected plants in the dry broccoli treatment was intermediate, similar to the number of microsclerotia. For maximal reductions in soilborne V. dahliae microsclerotia and the subsequent lower wilt incidence in cauliflower, the broccoli residue incorporation should occur when the temperatures are at least 20°C.

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Claims 15

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Dry and fresh broccoli significantly reduced V. dahliae microsclerotia in soil compared with unamended soil at all temperatures.

Treatment
both dry and fresh broccolie1
Interactor
Verticillium dahliaeVerticillium dahliaee0pathogen
Outcome
number of microsclerotia in soile1
Direction
reducede1
Qualifier
At all temperaturese1
Comparator
unamended soile1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Verticillium dahliae
Verticillium dahliae · InteractorHoward F. Schwartz · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The effects of dry, fresh, and no broccoli amendments on Verticillium dahliae microsclerotia in soil were evaluated at 10, 15, 20, 25, 30, and 35°C”

✓ located verbatim in the source · abstract, characters 0–147 · show in text ↑
e1

“At all temperatures, both dry and fresh broccoli significantly (P < 0.05) reduced the number of microsclerotia in soil compared with the unamended soil”

✓ located verbatim in the source · abstract, characters 623–774 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Fresh broccoli had a greater remissive effect on V. dahliae microsclerotia than dry broccoli.

Treatment
fresh broccolie2
Interactor
Verticillium dahliaeVerticillium dahliaee0pathogen
Outcome
remissive effecte2
Direction
greatere2
Comparator
dry broccolie2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Verticillium dahliae
Verticillium dahliae · InteractorHoward F. Schwartz · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The effects of dry, fresh, and no broccoli amendments on Verticillium dahliae microsclerotia in soil were evaluated at 10, 15, 20, 25, 30, and 35°C”

✓ located verbatim in the source · abstract, characters 0–147 · show in text ↑
e2

“fresh broccoli had a significantly (P < 0.05) greater remissive effect than dry broccoli”

✓ located verbatim in the source · abstract, characters 786–874 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

At 35°C, V. dahliae microsclerotia were reduced after 45 days in unamended soil.

Treatment
unamended soile3
Interactor
V. dahliaeVerticillium dahliaee3pathogen
Outcome
number of V. dahliae microsclerotiae3
Direction
reducede3
Qualifier
after 45 dayse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Verticillium dahliae
Verticillium dahliae · InteractorHoward F. Schwartz · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The effects of dry, fresh, and no broccoli amendments on Verticillium dahliae microsclerotia in soil were evaluated at 10, 15, 20, 25, 30, and 35°C”

✓ located verbatim in the source · abstract, characters 0–147 · show in text ↑
e3

“At 35°C, however, the number of V. dahliae microsclerotia were significantly (P < 0.05) reduced after 45 days in unamended soil”

✓ located verbatim in the source · abstract, characters 876–1003 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 35°C, both fresh and dry broccoli almost completely eliminated V. dahliae from soil.

Treatment
both fresh and dry broccolie4
Interactor
Verticillium dahliaeVerticillium dahliaee0pathogen
Outcome
eliminated the pathogen from soile4
Direction
almost completely eliminatede4
Comparator
unamended soile3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Verticillium dahliae
Verticillium dahliae · InteractorHoward F. Schwartz · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“The effects of dry, fresh, and no broccoli amendments on Verticillium dahliae microsclerotia in soil were evaluated at 10, 15, 20, 25, 30, and 35°C”

✓ located verbatim in the source · abstract, characters 0–147 · show in text ↑
e3

“At 35°C, however, the number of V. dahliae microsclerotia were significantly (P < 0.05) reduced after 45 days in unamended soil”

✓ located verbatim in the source · abstract, characters 876–1003 · show in text ↑
e4

“both fresh and dry broccoli almost completely eliminated the pathogen from soil”

✓ located verbatim in the source · abstract, characters 1005–1084 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Maximum reductions in microsclerotia from broccoli treatments occurred within 15 days regardless of temperature.

Treatment
treatments involving broccolie5
Outcome
maximum reductions in the number of microsclerotiae5
Qualifier
within 15 dayse5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e5

“Regardless of the temperature, maximum reductions in the number of microsclerotia in treatments involving broccoli occurred within 15 days, with fresh broccoli providing significantly (P < 0.05) greater reductions”

✓ located verbatim in the source · abstract, characters 1315–1528 · show in text ↑
Associationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Fresh broccoli gave greater microsclerotia reductions than dry broccoli.

Treatment
fresh broccolie5
Outcome
reductionse5
Direction
greatere5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e5

“Regardless of the temperature, maximum reductions in the number of microsclerotia in treatments involving broccoli occurred within 15 days, with fresh broccoli providing significantly (P < 0.05) greater reductions”

✓ located verbatim in the source · abstract, characters 1315–1528 · show in text ↑
Observationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Further reductions in microsclerotia were not significant in broccoli treatments at later sampling dates.

Treatment
broccoli treatmentse6
Outcome
Further reductions in the number of microsclerotiae6
Qualifier
at the subsequent sampling datese6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e6

“Further reductions in the number of microsclerotia were not significant in broccoli treatments at the subsequent sampling dates”

✓ located verbatim in the source · abstract, characters 1530–1657 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

In unamended soil, microsclerotia numbers changed little at all temperatures except 35°C.

Treatment
unamended soile7
Outcome
number of microsclerotiae7
Qualifier
at all temperatures except 35°Ce7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The effects of dry, fresh, and no broccoli amendments on Verticillium dahliae microsclerotia in soil were evaluated at 10, 15, 20, 25, 30, and 35°C”

✓ located verbatim in the source · abstract, characters 0–147 · show in text ↑
e7

“In unamended soil, the number of microsclerotia at all temperatures except 35°C changed little throughout the experiment”

✓ located verbatim in the source · abstract, characters 1659–1779 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The optimum temperatures for broccoli-mediated reductions of V. dahliae microsclerotia were 25 and 30°C.

Interactor
V. dahliaeVerticillium dahliaee6pathogen
Outcome
broccoli-mediated reductions in V. dahliae microsclerotiae6
Value
25 and 30°Ce6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Verticillium dahliae
Verticillium dahliae · InteractorHoward F. Schwartz · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e6

“The optimum temperatures for the broccoli-mediated reductions in V. dahliae microsclerotia in both experiments were 25 and 30°C”

✓ located verbatim in the source · abstract, characters 1781–1908 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cauliflower plants in the fresh broccoli treatment were taller than in other treatments.

Treatment
fresh broccoli treatmente7
Outcome
tallere7
Direction
higherinferrede7
Comparator
other treatmentse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e7

“Consistently, cauliflower plants in the fresh broccoli treatment were taller, had greater root and shoot weights, were significantly more robust, and had the least number of infected plants (number of microsclerotia was lower) than in other treatments”

✓ located verbatim in the source · abstract, characters 2045–2296 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cauliflower in the fresh broccoli treatment had greater root and shoot weights than in other treatments.

Treatment
fresh broccoli treatmente7
Outcome
root and shoot weightse7
Direction
greatere7
Comparator
other treatmentse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e7

“Consistently, cauliflower plants in the fresh broccoli treatment were taller, had greater root and shoot weights, were significantly more robust, and had the least number of infected plants (number of microsclerotia was lower) than in other treatments”

✓ located verbatim in the source · abstract, characters 2045–2296 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Cauliflower in the fresh broccoli treatment was significantly more robust than in other treatments.

Treatment
fresh broccoli treatmente7
Outcome
robuste7
Direction
higherinferrede7
Comparator
other treatmentse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e7

“Consistently, cauliflower plants in the fresh broccoli treatment were taller, had greater root and shoot weights, were significantly more robust, and had the least number of infected plants (number of microsclerotia was lower) than in other treatments”

✓ located verbatim in the source · abstract, characters 2045–2296 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cauliflower in the fresh broccoli treatment had the least number of infected plants.

Treatment
fresh broccoli treatmente7
Outcome
number of infected plantse7
Direction
leaste7
Comparator
other treatmentse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e7

“Consistently, cauliflower plants in the fresh broccoli treatment were taller, had greater root and shoot weights, were significantly more robust, and had the least number of infected plants (number of microsclerotia was lower) than in other treatments”

✓ located verbatim in the source · abstract, characters 2045–2296 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The dry broccoli treatment gave an intermediate number of infected cauliflower plants.

Treatment
dry broccoli treatmente7
Outcome
number of infected plantse7
Qualifier
intermediatee7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e6

“Consistently, cauliflower plants in the fresh broccoli treatment were taller, had greater root and shoot weights, were significantly more robust, and had the least number of infected plants (number of microsclerotia was lower) than in other treatments”

✓ located verbatim in the source · abstract, characters 2045–2296 · show in text ↑
e7

“The number of infected plants in the dry broccoli treatment was intermediate, similar to the number of microsclerotia”

✓ located verbatim in the source · abstract, characters 2298–2415 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Broccoli residue should be incorporated when temperatures are at least 20°C to maximize reductions in V. dahliae microsclerotia and wilt in cauliflower.

Interactor
V. dahliaeVerticillium dahliaee0pathogen
Outcome
broccoli residue incorporation should occur when the temperatures are at least 20°Ce0
Qualifier
For maximal reductionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. botrytis
Brassica oleracea var. botrytis · TaxonSusan Slater · CC BY-SA 4.0 · Wikimedia Commons ↗
Verticillium dahliae
Verticillium dahliae · InteractorHoward F. Schwartz · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“For maximal reductions in soilborne V. dahliae microsclerotia and the subsequent lower wilt incidence in cauliflower, the broccoli residue incorporation should occur when the temperatures are at least 20°C”

✓ located verbatim in the source · abstract, characters 2417–2622 · show in text ↑

Brassica oleracea var. italica · disease · Brassica oleracea (wild cabbage)Positive and negative results

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.