Abstract
Multidrug and toxic compound extrusion (MATE) proteins comprise the most recently identified family of multidrug transporters. In plants, the numbers of MATE proteins has undergone a remarkable expansion, underscoring the importance of these transporters within this kingdom. Here, we describe the identification and characterization of Activated Disease Susceptibility 1 (ADS1) which encodes a putative MATE transport protein. An activation tagging screen uncovered the ads1-Dominant (ads1-D) mutant, which was subsequently characterized by molecular, genetic and biochemical approaches. The ads1-D mutant was compromised in both basal and nonhost resistance against microbial pathogens. Further, plant defence responses conferred by RPS4 were also disabled in ads1-D plants. By contrast, depletion of ADS1 transcripts by RNA-interference (RNAi) promoted basal disease resistance. Unexpectedly, ads1-D plants were found to constitutively accumulate reactive oxygen intermediates (ROIs). However, analysis of ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants indicated that an increase in ROIs did not impact ads1-D-mediated disease susceptibility. Our findings imply that ADS1 negatively regulates the accumulation of the plant immune activator salicylic acid (SA) and cognate Pathogenesis-Related 1 (PR1) gene expression. Collectively, these data highlight an important role for MATE proteins in the establishment of plant disease resistance.
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Claims 8
Claim · model-written summary
ADS1 encodes a putative MATE transport protein.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Gene
- Activated Disease Susceptibility 1 (ADS1)e0
- Outcome
- putative MATE transport proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Activated Disease Susceptibility 1 (ADS1) which encodes a putative MATE transport protein”
e1“ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants”
Claim · model-written summary
The ads1-D mutant had compromised basal and nonhost resistance against microbial pathogens.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- ads1-D mutante0
- Interactor
- microbial pathogense0pathogen
- Outcome
- basal and nonhost resistancee0
- Direction
- compromisede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ads1-D mutant was compromised in both basal and nonhost resistance against microbial pathogens.”
e1“ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants”
Claim · model-written summary
RPS4-conferred defence responses were disabled in ads1-D plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- ads1-De0
- Gene
- RPS4e0
- Outcome
- plant defence responsese0
- Direction
- disablede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plant defence responses conferred by RPS4 were also disabled in ads1-D plants”
e1“ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants”
Claim · model-written summary
ADS1 transcript depletion by RNA interference promoted basal disease resistance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- depletion of ADS1 transcripts by RNA-interference (RNAi)e0
- Gene
- ADS1e0
- Outcome
- basal disease resistancee0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“depletion of ADS1 transcripts by RNA-interference (RNAi) promoted basal disease resistance”
e1“ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants”
Claim · model-written summary
ads1-D plants constitutively accumulated reactive oxygen intermediates.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- ads1-De0
- Outcome
- reactive oxygen intermediates (ROIs)e0
- Direction
- accumulatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ads1-D plants were found to constitutively accumulate reactive oxygen intermediates (ROIs)”
e1“ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants”
Claim · model-written summary
In atrboh double and triple mutants, increased reactive oxygen intermediates did not affect ads1-D-mediated disease susceptibility.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- double and triple mutantse0
- Gene
- atrbohe0
- Compound
- ROIse0
- Outcome
- ads1-D-mediated disease susceptibilitye0
- Direction
- did not impacte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“analysis of ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants indicated that an increase in ROIs did not impact ads1-D-mediated disease susceptibility”
Claim · model-written summary
ADS1 appears to negatively regulate salicylic acid accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- ADS1e0
- Outcome
- accumulation of the plant immune activator salicylic acid (SA)e0
- Direction
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ADS1 negatively regulates the accumulation of the plant immune activator salicylic acid (SA) and cognate Pathogenesis-Related 1 (PR1) gene expression”
e1“ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants”
Claim · model-written summary
ADS1 appears to negatively regulate PR1 gene expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- ADS1e0
- Outcome
- Pathogenesis-Related 1 (PR1) gene expressione0
- Direction
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ADS1 negatively regulates the accumulation of the plant immune activator salicylic acid (SA) and cognate Pathogenesis-Related 1 (PR1) gene expression”
e1“ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants”
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