Abstract
UV-B radiation has previously been reported to reduce growth, flowering, and net photosynthesis. The present study examines the effect of UV-B radiation on isolated chloroplast of 7-10 day old pea seedlings. Amount of ({sup 3}H)-Leu incorporated into isolated chloroplasts was measured in the presence or absence of UV-B exposure. Preliminary experiments show a 30% inhibition of protein synthesis in isolated chloroplasts after only 20 mins of UV-B exposure (6.9 J/m{sup 2}/30 min). Percent inhibition of chloroplast translation is directly correlated with UV-B exposure over a 60 min time span. Preliminary studies also show no change in both cold and radiolabeled protein profiles as expressed on 1-D PAGE and autofluorography. Comparative studies on the sensitivity of e{sup {minus}} flow vs protein synthesis following UV-B exposure are underway. Further work on the role of oxygen free radicals and the specific site of action of UV-B damage to the translation machinery of chloroplasts will be discussed.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 3
Claim · model-written summary
UV-B exposure inhibited protein synthesis in isolated pea chloroplasts by 30% after 20 minutes.
- Taxon
- Pisum sativum
Lathyrus oleraceust0 - Organ / stage
- isolated chloroplastse0
- Treatment
- UV-B exposure (6.9 J/m{sup 2}/30 min)e0
- Outcome
- protein synthesise0
- Direction
- inhibitione0
- Value
- 30%e0
- Qualifier
- after only 20 minse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preliminary experiments show a 30% inhibition of protein synthesis in isolated chloroplasts after only 20 mins of UV-B exposure (6.9 J/m{sup 2}/30 min).”
t0“Pisum sativum”
Claim · model-written summary
Inhibition of chloroplast translation correlated directly with UV-B exposure over 60 minutes.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- UV-B exposuree0
- Outcome
- Percent inhibition of chloroplast translatione0
- Direction
- higherinferrede0
- Qualifier
- 60 min time spane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Percent inhibition of chloroplast translation is directly correlated with UV-B exposure over a 60 min time span.”
t0“Pisum sativum”
Claim · model-written summary
Preliminary studies found no change in cold and radiolabeled protein profiles on 1-D PAGE and autofluorography.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- UV-B exposuree1
- Outcome
- cold and radiolabeled protein profilese0
- Direction
- no changee0
- Comparator
- absence of UV-B exposuree1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preliminary studies also show no change in both cold and radiolabeled protein profiles as expressed on 1-D PAGE and autofluorography.”
e1“presence or absence of UV-B exposure”
t0“Pisum sativum”
The same questions in other papers
uv · quality: protein · Lathyrus oleraceus (pea)Results agree
2 papers in the same direction as this paper
- negative
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.