Abstract
Metals exert important functions in the chloroplast of plants, where they act as cofactors and catalysts in the photosynthetic electron transport chain. In particular, manganese (Mn) has a key function because of its indispensable role in the water-splitting reaction of photosystem II (PSII). More and better knowledge is required on how the various complexes of PSII are affected in response to, for example, nutritional disorders and other environmental stress conditions. We here present, to our knowledge, a new method that allows the analysis of metal binding in intact photosynthetic complexes of barley (Hordeum vulgare) thylakoids. The method is based on size exclusion chromatography coupled to inductively coupled plasma triple-quadrupole mass spectrometry. Proper fractionation of PSII super- and subcomplexes was achieved by critical selection of elution buffers, detergents for protein solubilization, and stabilizers to maintain complex integrity. The applicability of the method was shown by quantification of Mn binding in PSII from thylakoids of two barley genotypes with contrasting Mn efficiency exposed to increasing levels of Mn deficiency. The amount of PSII supercomplexes was drastically reduced in response to Mn deficiency. The Mn efficient genotype bound significantly more Mn per unit of PSII under control and mild Mn deficiency conditions than the inefficient genotype, despite having lower or similar total leaf Mn concentrations. It is concluded that the new method facilitates studies of the internal use of Mn and other biometals in various PSII complexes as well as their relative dynamics according to changes in environmental conditions.
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Claims 4
Claim · model-written summary
A size exclusion and ICP-MS method was shown to quantify Mn binding in PSII of barley thylakoids from two genotypes under increasing Mn deficiency.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- increasing levels of Mn deficiencye1
- Outcome
- quantification of Mn binding in PSIIe1
- Comparator
- two barley genotypes with contrasting Mn efficiencye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“analysis of metal binding in intact photosynthetic complexes of barley (Hordeum vulgare) thylakoids”
e1“The applicability of the method was shown by quantification of Mn binding in PSII from thylakoids of two barley genotypes with contrasting Mn efficiency exposed to increasing levels of Mn deficiency”
Claim · model-written summary
Mn deficiency drastically reduced the amount of PSII supercomplexes in barley.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- Mn deficiencye2
- Outcome
- amount of PSII supercomplexese2
- Direction
- reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“analysis of metal binding in intact photosynthetic complexes of barley (Hordeum vulgare) thylakoids”
e2“The amount of PSII supercomplexes was drastically reduced in response to Mn deficiency”
Claim · model-written summary
The Mn efficient barley genotype bound significantly more Mn per unit of PSII than the inefficient genotype under control and mild Mn deficiency.
- Taxon
- barleyHordeum vulgaree0
- Material
- Mn efficient genotypee3
- Treatment
- control and mild Mn deficiency conditionse3
- Outcome
- Mn per unit of PSIIe3
- Direction
- moree3
- Comparator
- inefficient genotypee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“analysis of metal binding in intact photosynthetic complexes of barley (Hordeum vulgare) thylakoids”
e3“The Mn efficient genotype bound significantly more Mn per unit of PSII under control and mild Mn deficiency conditions than the inefficient genotype, despite having lower or similar total leaf Mn concentrations”
Claim · model-written summary
The Mn efficient barley genotype had lower or similar total leaf Mn concentrations than the inefficient genotype.
- Taxon
- barleyHordeum vulgaree0
- Material
- Mn efficient genotypee3
- Organ / stage
- leafe3
- Outcome
- total leaf Mn concentrationse3
- Direction
- lower or similare3
- Comparator
- inefficient genotypee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“analysis of metal binding in intact photosynthetic complexes of barley (Hordeum vulgare) thylakoids”
e3“The Mn efficient genotype bound significantly more Mn per unit of PSII under control and mild Mn deficiency conditions than the inefficient genotype, despite having lower or similar total leaf Mn concentrations”
The same questions in other papers
manganese atom · physiology: photosynthesis · Hordeum vulgare (barley)Results agree
4 papers in the same direction as this paper
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- positive
1 paper more in this group
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