Abstract
Phosphatase activity in cultured tobacco cells XD-6 increased remarkably under phosphate-deficient culture conditions. Such an increase did not occur in the activities of α-amylase, β-galactosidase, succinate dehydrogenase and catalase under the same culture conditions. By replenishment with Pi, the increase in total phosphatase activity was suppressed and the specific activity was reduced to a low level. The suppression of increase in the activity resulted form a repression of de novo synthesis of the enzyme. Added Pi also suppressed the release of phosphatase into the culture medium. The effect of Pi was found to be greater than the effect on the increase in the intracellular activity. At least three phosphatases were extracted from XD-6 cells. One of the enzymes increased when the phosphatase synthesis was increased by phosphate deficiency.
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Claims 9
Claim · model-written summary
Phosphate deficiency markedly increased phosphatase activity.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- XD-6e0
- Treatment
- phosphate-deficient culture conditionse0
- Outcome
- Phosphatase activitye0
- Direction
- increased remarkablye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phosphatase activity in cultured tobacco cells XD-6 increased remarkably under phosphate-deficient culture conditions.”
Claim · model-written summary
Phosphate deficiency did not increase α-amylase, β-galactosidase, succinate dehydrogenase or catalase activities.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- same culture conditionse0
- Outcome
- activities of α-amylase, β-galactosidase, succinate dehydrogenase and catalasee0
- Direction
- did not occure0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Such an increase did not occur in the activities of α-amylase, β-galactosidase, succinate dehydrogenase and catalase under the same culture conditions.”
t0“Regulation of phosphatase synthesis by orthophosphate in cultured tobacco cells”
Claim · model-written summary
Pi replenishment suppressed the total phosphatase activity increase.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- replenishment with Pie0
- Outcome
- increase in total phosphatase activitye0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By replenishment with Pi, the increase in total phosphatase activity was suppressed and the specific activity was reduced to a low level.”
t0“Regulation of phosphatase synthesis by orthophosphate in cultured tobacco cells”
Claim · model-written summary
Pi replenishment reduced specific phosphatase activity to a low level.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- replenishment with Pie0
- Outcome
- specific activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By replenishment with Pi, the increase in total phosphatase activity was suppressed and the specific activity was reduced to a low level.”
t0“Regulation of phosphatase synthesis by orthophosphate in cultured tobacco cells”
Claim · model-written summary
The suppression was attributed to repression of new enzyme synthesis.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- suppression of increase in the activitye0
- Qualifier
- repression of de novo synthesis of the enzymee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The suppression of increase in the activity resulted form a repression of de novo synthesis of the enzyme.”
t0“Regulation of phosphatase synthesis by orthophosphate in cultured tobacco cells”
Claim · model-written summary
Added Pi suppressed phosphatase release into culture medium.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Added Pie0
- Outcome
- release of phosphatase into the culture mediume0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Added Pi also suppressed the release of phosphatase into the culture medium.”
t0“Regulation of phosphatase synthesis by orthophosphate in cultured tobacco cells”
Claim · model-written summary
Pi had a greater effect on phosphatase release than on intracellular activity increase.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- effect of Pie0
- Direction
- greatere0
- Comparator
- increase in the intracellular activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of Pi was found to be greater than the effect on the increase in the intracellular activity.”
t0“Regulation of phosphatase synthesis by orthophosphate in cultured tobacco cells”
Claim · model-written summary
At least three phosphatases were extracted from XD-6 cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- XD-6e0
- Outcome
- At least three phosphatasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At least three phosphatases were extracted from XD-6 cells.”
t0“Regulation of phosphatase synthesis by orthophosphate in cultured tobacco cells”
Claim · model-written summary
One extracted phosphatase increased under phosphate deficiency.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- phosphate deficiencye0
- Outcome
- One of the enzymese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the enzymes increased when the phosphatase synthesis was increased by phosphate deficiency.”
t0“Regulation of phosphatase synthesis by orthophosphate in cultured tobacco cells”
The same questions in other papers
in vitro · physiology: antioxidant enzymes · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.