Abstract
Bacillus subtilis B47 is an endophytic bacterium of tomato,and produces a substance to inhibit some plant pathogens.The antimicrobial substance was extracted and purified by hydrochloric acid precipitation,methanol extraction,silica gel column chromatography,gel filtration chromatography and reversed phase high performance liquid chromatography.The purified substance was stable to alkaline,heat,protease and UV.It could dissolve in methanol.The substance showed positive reaction to biuret and negative reaction to ninhydrin,but showed positive reaction to ninhydrin when it was hydrolyzed with acid.All the data above indicated that the substance could be ring peptide.The growth of 14 plant pathogens could be inhibit by the substance,among which,the pathogens of the northern corn leaf blight,southern corn leaf blight and banana leaf spot were inhibited strongly,while no inhibition to the tested bacterial pathogens.Control efficacy of antimicrobial substance produced by Bacillus subtilis B47 at 0.3 g·L-1 on the southern corn leaf blight in field was 53.05%.
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Claims 12
Claim · model-written summary
Bacillus subtilis B47 antimicrobial substance was stable to alkaline,heat,protease and UV.
- Taxon
- Corn
Zea mays subsp. mayst0 - Interactor
- Bacillus subtilis B47
Bacillus subtilist1biocontrol_agent - Outcome
- stable to alkaline,heat,protease and UVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purified substance was stable to alkaline,heat,protease and UV.It could dissolve in methanol.The substance showed positive reaction to biuret and negative reaction to ninhydrin,but showed positive reaction to ninhydrin when it was hydrolyzed with acid.All the data above indicated that the substance could be ring peptide.”
t0“Corn”
t1“Bacillus subtilis B47”
Claim · model-written summary
Bacillus subtilis B47 antimicrobial substance was dissolve in methanol.
- Taxon
- CornZea mays subsp. mayst0
- Interactor
- Bacillus subtilis B47Bacillus subtilist1biocontrol_agent
- Outcome
- dissolve in methanole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purified substance was stable to alkaline,heat,protease and UV.It could dissolve in methanol.The substance showed positive reaction to biuret and negative reaction to ninhydrin,but showed positive reaction to ninhydrin when it was hydrolyzed with acid.All the data above indicated that the substance could be ring peptide.”
t0“Corn”
t1“Bacillus subtilis B47”
Claim · model-written summary
Bacillus subtilis B47 antimicrobial substance was positive reaction to biuret.
- Taxon
- CornZea mays subsp. mayst0
- Interactor
- Bacillus subtilis B47Bacillus subtilist1biocontrol_agent
- Outcome
- positive reaction to biurete0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purified substance was stable to alkaline,heat,protease and UV.It could dissolve in methanol.The substance showed positive reaction to biuret and negative reaction to ninhydrin,but showed positive reaction to ninhydrin when it was hydrolyzed with acid.All the data above indicated that the substance could be ring peptide.”
t0“Corn”
t1“Bacillus subtilis B47”
Claim · model-written summary
Bacillus subtilis B47 antimicrobial substance was negative reaction to ninhydrin.
- Taxon
- CornZea mays subsp. mayst0
- Interactor
- Bacillus subtilis B47Bacillus subtilist1biocontrol_agent
- Outcome
- negative reaction to ninhydrine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purified substance was stable to alkaline,heat,protease and UV.It could dissolve in methanol.The substance showed positive reaction to biuret and negative reaction to ninhydrin,but showed positive reaction to ninhydrin when it was hydrolyzed with acid.All the data above indicated that the substance could be ring peptide.”
t0“Corn”
t1“Bacillus subtilis B47”
Claim · model-written summary
Bacillus subtilis B47 antimicrobial substance was positive reaction to ninhydrin when it was hydrolyzed with acid.
- Taxon
- CornZea mays subsp. mayst0
- Interactor
- Bacillus subtilis B47Bacillus subtilist1biocontrol_agent
- Outcome
- positive reaction to ninhydrin when it was hydrolyzed with acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purified substance was stable to alkaline,heat,protease and UV.It could dissolve in methanol.The substance showed positive reaction to biuret and negative reaction to ninhydrin,but showed positive reaction to ninhydrin when it was hydrolyzed with acid.All the data above indicated that the substance could be ring peptide.”
t0“Corn”
t1“Bacillus subtilis B47”
Claim · model-written summary
The antimicrobial substance was inferred to be a ring peptide.
- Taxon
- CornZea mays subsp. mayst0
- Outcome
- ring peptidee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purified substance was stable to alkaline,heat,protease and UV.It could dissolve in methanol.The substance showed positive reaction to biuret and negative reaction to ninhydrin,but showed positive reaction to ninhydrin when it was hydrolyzed with acid.All the data above indicated that the substance could be ring peptide.”
t0“Corn”
Claim · model-written summary
The substance inhibit 14 plant pathogens.
- Taxon
- CornZea mays subsp. mayst0
- Interactor
- 14 plant pathogense0pathogen
- Outcome
- 14 plant pathogense0
- Direction
- inhibite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of 14 plant pathogens could be inhibit by the substance,among which,the pathogens of the northern corn leaf blight,southern corn leaf blight and banana leaf spot were inhibited strongly,while no inhibition to the tested bacterial pathogens.”
t0“Corn”
Claim · model-written summary
The substance inhibited strongly northern corn leaf blight.
- Taxon
- CornZea mays subsp. mayst0
- Interactor
- northern corn leaf blight
Exserohilum turcicume0pathogen - Outcome
- northern corn leaf blighte0
- Direction
- inhibited stronglye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of 14 plant pathogens could be inhibit by the substance,among which,the pathogens of the northern corn leaf blight,southern corn leaf blight and banana leaf spot were inhibited strongly,while no inhibition to the tested bacterial pathogens.”
t0“Corn”
Claim · model-written summary
The substance inhibited strongly southern corn leaf blight.
- Taxon
- CornZea mays subsp. mayst0
- Interactor
- southern corn leaf blighte0pathogen
- Outcome
- southern corn leaf blighte0
- Direction
- inhibited stronglye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of 14 plant pathogens could be inhibit by the substance,among which,the pathogens of the northern corn leaf blight,southern corn leaf blight and banana leaf spot were inhibited strongly,while no inhibition to the tested bacterial pathogens.”
t0“Corn”
Claim · model-written summary
The substance inhibited strongly banana leaf spot.
Verbatim
e0“The growth of 14 plant pathogens could be inhibit by the substance,among which,the pathogens of the northern corn leaf blight,southern corn leaf blight and banana leaf spot were inhibited strongly,while no inhibition to the tested bacterial pathogens.”
Claim · model-written summary
The substance no inhibition tested bacterial pathogens.
- Taxon
- CornZea mays subsp. mayst0
- Interactor
- tested bacterial pathogense0pathogen
- Outcome
- tested bacterial pathogense0
- Direction
- no inhibitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of 14 plant pathogens could be inhibit by the substance,among which,the pathogens of the northern corn leaf blight,southern corn leaf blight and banana leaf spot were inhibited strongly,while no inhibition to the tested bacterial pathogens.”
t0“Corn”
Claim · model-written summary
B. subtilis B47 antimicrobial substance controlled southern corn leaf blight by 53.05% in the field.
- Taxon
- CornZea mays subsp. mayst0
- Treatment
- antimicrobial substance produced by Bacillus subtilis B47 at 0.3 g·L-1e0
- Interactor
- southern corn leaf blighte0pathogen
- Second interactor
- Bacillus subtilis B47Bacillus subtilise0biocontrol_agent
- Outcome
- Control efficacye0
- Value
- 53.05%e0
- Setting
- fielde0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Control efficacy of antimicrobial substance produced by Bacillus subtilis B47 at 0.3 g·L-1 on the southern corn leaf blight in field was 53.05%.”
t0“Corn”
The same questions in other papers
antimicrobial agent · control · Zea mays (maize)Positive and negative results
1 in the opposite direction
- negative
Bacillus subtilis · control · Zea mays (maize)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.