Abstract
In this study, we characterized the semi-dominant mutant nls1-1D (necrotic leaf sheath 1) of rice, which displays spontaneous lesions, specifically on leaf sheaths, with a developmental pattern. nls1-1D plants also exhibited constitutively activated defense responses, including extensive cell death, excess hydrogen peroxide and salicylic acid (SA) accumulation, up-regulated expressions of pathogenesis-related genes, and enhanced resistance to bacterial pathogens. Map-based cloning revealed that NLS1 encodes a typical CC-NB-LRR-type protein in rice. The nls1-1D mutation causes a S367N substitution in the non-conserved region close to the GLPL motif of the NB domain. An adjacent S366T substitution was found in another semi-dominant mutant, nls1-2D, which exhibited the same phenotypes as nls1-1D. Combined analyses of wild-type plants transformed with the mutant NLS1 gene (nls1-1D), NLS1 RNAi and over-expression transgenic lines showed that nls1-2D is allelic to nls1-1D, and both mutations may cause constitutive auto-activation of the NLS1 R protein. Further real-time PCR analysis revealed that NLS1 is expressed constitutively in an age-dependent manner. In addition, because the morphology and constitutive defense responses of nls1-1D were not suppressed by blocking SA or NPR1 transcript accumulation, we suggest that NLS1 mediates both SA and NPR1-independent defense signaling pathways in rice.
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Claims 15
Claim · model-written summary
The rice nls1-1D mutant develops spontaneous lesions on leaf sheaths in a developmental pattern.
- Taxon
- rice
Oryza sativae0 - Material
- nls1-1De0
- Organ / stage
- leaf sheathse0
- Outcome
- spontaneous lesionse0
- Qualifier
- with a developmental patterne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“semi-dominant mutant nls1-1D (necrotic leaf sheath 1) of rice, which displays spontaneous lesions, specifically on leaf sheaths, with a developmental pattern”
Claim · model-written summary
The rice nls1-1D mutant exhibited extensive cell death.
- Taxon
- riceOryza sativat0
- Material
- nls1-1De0
- Outcome
- extensive cell deathe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nls1-1D plants also exhibited constitutively activated defense responses, including extensive cell death”
t0“rice”
Claim · model-written summary
The rice nls1-1D mutant accumulated excess hydrogen peroxide and salicylic acid.
- Taxon
- riceOryza sativat0
- Material
- nls1-1De0
- Outcome
- excess hydrogen peroxide and salicylic acid (SA) accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nls1-1D plants also exhibited constitutively activated defense responses, including extensive cell death, excess hydrogen peroxide and salicylic acid (SA) accumulation”
t0“rice”
Claim · model-written summary
The rice nls1-1D mutant had increased expression of pathogenesis-related genes.
- Taxon
- riceOryza sativat0
- Material
- nls1-1De0
- Gene
- pathogenesis-related genese0
- Outcome
- expressions of pathogenesis-related genese0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nls1-1D plants also exhibited constitutively activated defense responses, including extensive cell death, excess hydrogen peroxide and salicylic acid (SA) accumulation, up-regulated expressions of pathogenesis-related genes”
t0“rice”
Claim · model-written summary
The rice nls1-1D mutant had enhanced resistance to bacterial pathogens.
- Taxon
- riceOryza sativat0
- Material
- nls1-1De0
- Interactor
- bacterial pathogense0pathogen
- Outcome
- resistance to bacterial pathogense0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nls1-1D plants also exhibited constitutively activated defense responses, including extensive cell death, excess hydrogen peroxide and salicylic acid (SA) accumulation, up-regulated expressions of pathogenesis-related genes, and enhanced resistance to bacterial pathogens”
t0“rice”
Claim · model-written summary
NLS1 encodes a typical CC-NB-LRR-type protein in rice.
- Taxon
- riceOryza sativae0
- Gene
- NLS1e0
- Outcome
- encodes a typical CC-NB-LRR-type proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NLS1 encodes a typical CC-NB-LRR-type protein in rice”
Claim · model-written summary
The nls1-1D mutation causes an S367N substitution near the GLPL motif.
- Taxon
- riceOryza sativat0
- Gene
- nls1-1De0
- Outcome
- S367N substitutione0
- Qualifier
- in the non-conserved region close to the GLPL motif of the NB domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nls1-1D mutation causes a S367N substitution in the non-conserved region close to the GLPL motif of the NB domain”
t0“rice”
Claim · model-written summary
The nls1-2D mutant carries an adjacent S366T substitution.
- Taxon
- riceOryza sativat0
- Material
- nls1-2De0
- Outcome
- S366T substitutione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An adjacent S366T substitution was found in another semi-dominant mutant, nls1-2D”
t0“rice”
Claim · model-written summary
The nls1-2D mutant had the same phenotypes as nls1-1D.
- Taxon
- riceOryza sativat0
- Material
- nls1-2De0
- Outcome
- same phenotypese0
- Comparator
- nls1-1De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nls1-2D, which exhibited the same phenotypes as nls1-1D”
t0“rice”
Claim · model-written summary
nls1-2D is allelic to nls1-1D.
- Taxon
- riceOryza sativat0
- Gene
- nls1-2De0
- Outcome
- allelic to nls1-1De0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nls1-2D is allelic to nls1-1D”
t0“rice”
Claim · model-written summary
Both NLS1 mutations may cause constitutive auto-activation of the NLS1 R protein.
- Taxon
- riceOryza sativat0
- Gene
- NLS1 R proteine0
- Outcome
- constitutive auto-activatione0
- Qualifier
- may causee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both mutations may cause constitutive auto-activation of the NLS1 R protein”
t0“rice”
Claim · model-written summary
NLS1 expression is constitutive and age-dependent.
- Taxon
- riceOryza sativat0
- Gene
- NLS1e0
- Outcome
- expressed constitutively in an age-dependent mannere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NLS1 is expressed constitutively in an age-dependent manner”
t0“rice”
Claim · model-written summary
Blocking SA did not suppress nls1-1D morphology or constitutive defense responses.
- Taxon
- riceOryza sativat0
- Material
- nls1-1De0
- Treatment
- blocking SAe0
- Outcome
- morphology and constitutive defense responsese0
- Direction
- not suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the morphology and constitutive defense responses of nls1-1D were not suppressed by blocking SA”
t0“rice”
Claim · model-written summary
Blocking NPR1 transcript accumulation did not suppress nls1-1D morphology or constitutive defense responses.
- Taxon
- riceOryza sativat0
- Material
- nls1-1De0
- Treatment
- blocking SA or NPR1 transcript accumulatione0
- Gene
- NPR1e0
- Outcome
- morphology and constitutive defense responsese0
- Direction
- not suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the morphology and constitutive defense responses of nls1-1D were not suppressed by blocking SA or NPR1 transcript accumulation”
t0“rice”
Claim · model-written summary
NLS1 may mediate SA- and NPR1-independent defense signaling pathways in rice.
- Taxon
- riceOryza sativae0
- Gene
- NLS1e0
- Outcome
- defense signaling pathwayse0
- Qualifier
- SA and NPR1-independente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NLS1 mediates both SA and NPR1-independent defense signaling pathways in rice”
The same questions in other papers
salicylic acid · disease · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 26 in the opposite direction
- positive
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See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.