Abstract
To dissect the cellular response to water stress and compare changes induced as a generalized response with those involved in tolerance/acclimation mechanisms, we analyzed changes in two-dimensional electrophoretic patterns of in vivo [35S]methionine-labeled polypeptides of cultured potato (Solanum tuberosum) cells after gradual and long exposure to polyethylene glycol (PEG)- mediated low water potential versus those induced in cells abruptly exposed to the same stress intensity. Protein synthesis was not inhibited by gradual stress imposition, and the expression of 17 proteins was induced in adapted cells. Some polypeptides were inducible under mild stress conditions (5% PEG) and accumulated further when cells were exposed to a higher stress intensity (10 and 20% PEG). The synthesis of another set of polypeptides was up-regulated only when more severe water-stress conditions were applied, suggesting that plant cells were able to monitor different levels of stress intensity and modulate gene expression accordingly. In contrast, in potato cells abruptly exposed to 20% PEG, protein synthesis was strongly inhibited. Nevertheless, a large set of polypeptides was identified whose expression was increased. Most of these polypeptides were not induced in adapted cells, but many of them were common to those observed in abscisic acid (ABA)-treated cells. These data, along with the finding that cellular ABA content increased in PEG-shocked cells but not in PEG-adapted cells, suggested that this hormone is mainly involved in the rapid response to stress rather than long-term adaptation. A further group of proteins included those induced after long exposure to both water stress and shock. Western blot analysis revealed that osmotin was one protein belonging to this common group. This class may represent induced proteins that accumulate specifically in response to low water potential and that are putatively involved in the maintenance of cellular homeostasis under prolonged stress.
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Claims 12
Claim · model-written summary
Gradual water stress did not inhibit protein synthesis in potato cells.
- Taxon
- Potato
Solanum tuberosumt0 - Treatment
- gradual stress impositione0
- Outcome
- Protein synthesise0
- Direction
- not inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein synthesis was not inhibited by gradual stress imposition”
t0“Potato (Solanum tuberosum)”
Claim · model-written summary
Seventeen proteins were induced in adapted potato cells.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- expression of 17 proteinse0
- Direction
- inducede0
- Value
- 17 proteinse0
- Qualifier
- adapted cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum)”
e0“the expression of 17 proteins was induced in adapted cells”
Claim · model-written summary
Some polypeptides were induced under 5% PEG and accumulated further at 10 and 20% PEG.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- 5% PEGe0
- Outcome
- Some polypeptidese0
- Direction
- accumulated furthere0
- Comparator
- 10 and 20% PEGe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum)”
e0“Some polypeptides were inducible under mild stress conditions (5% PEG) and accumulated further when cells were exposed to a higher stress intensity (10 and 20% PEG)”
Claim · model-written summary
Another group of polypeptides was up-regulated only under more severe water stress.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- more severe water-stress conditionse0
- Outcome
- synthesis of another set of polypeptidese0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum)”
e0“The synthesis of another set of polypeptides was up-regulated only when more severe water-stress conditions were applied”
Claim · model-written summary
Abrupt exposure to 20% PEG strongly inhibited protein synthesis in potato cells.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- 20% PEGe0
- Outcome
- protein synthesise0
- Direction
- strongly inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in potato cells abruptly exposed to 20% PEG, protein synthesis was strongly inhibited”
Claim · model-written summary
Despite abrupt PEG exposure, expression increased for a large group of polypeptides.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- expressione0
- Direction
- increasede0
- Qualifier
- a large set of polypeptidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum)”
e0“a large set of polypeptides was identified whose expression was increased”
Claim · model-written summary
Most polypeptides increased by shock were not induced in adapted cells.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- polypeptidese0
- Direction
- not inducede0
- Qualifier
- adapted cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum)”
e0“Most of these polypeptides were not induced in adapted cells”
Claim · model-written summary
Many shock-induced polypeptides also occurred in ABA-treated cells.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- abscisic acid (ABA)-treatede0
- Outcome
- common to those observed in abscisic acid (ABA)-treated cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum)”
e0“many of them were common to those observed in abscisic acid (ABA)-treated cells”
Claim · model-written summary
Cellular ABA content increased after PEG shock but not after PEG adaptation.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- PEG-shockede0
- Outcome
- cellular ABA contente0
- Direction
- increasede0
- Comparator
- PEG-adapted cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum)”
e0“cellular ABA content increased in PEG-shocked cells but not in PEG-adapted cells”
Claim · model-written summary
ABA was interpreted as chiefly involved in the rapid stress response rather than long-term adaptation.
- Taxon
- PotatoSolanum tuberosumt0
- Compound
- abscisic acid (ABA)e1
- Outcome
- rapid response to stresse0
- Qualifier
- rather than long-term adaptatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum)”
e0“this hormone is mainly involved in the rapid response to stress rather than long-term adaptation”
e1“abscisic acid (ABA)-treated cells”
Claim · model-written summary
A group of proteins was induced after prolonged water stress and after shock.
- Taxon
- PotatoSolanum tuberosumt0
- Outcome
- proteins included those inducede0
- Qualifier
- long exposure to both water stress and shocke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum)”
e0“A further group of proteins included those induced after long exposure to both water stress and shock”
Claim · model-written summary
Osmotin belonged to the protein group common to prolonged stress and shock.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- osmotine0
- Outcome
- one protein belonging to this common groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Potato (Solanum tuberosum)”
e0“Western blot analysis revealed that osmotin was one protein belonging to this common group”
The same questions in other papers
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drought · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
7 papers in the same direction as this paper · 8 in the opposite direction
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12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.