Abstract
Wheat (Triticum aestivum L.) occupies an important place among cereals and it serves as a staple food for about 35% of the world population. Crop plants experience various stresses during their life cycle under high or low temperature, drought, and salinity conditions. Among these stresses, heat stress is getting alarming as a result of global warming. Therefore, research on the mechanism of high temperature stress in plants has the importance for future generations. The present investigation was aimed at studying the effect of heat stress and revival on antioxidative system and polypeptide pattern in the leaves of wheat seedlings. Four wheat genotypes, i.e., two tolerant (HW-2045 and WH-1021) and two susceptible (HS-277 and WH-147) were selected and the enzymes of antioxidant system, levels of antioxidant metabolites and pattern of polypeptide were observed. The production of H2O2 was higher in the leaves of susceptible and tolerant genotypes under heat stress conditions. However, its level was significantly higher in susceptible as compared to tolerant genotypes. There was enhancement in the activities of antioxidative enzymes, viz. CAT, POX, GR and APX in the leaves of tolerant and susceptible genotypes under heat stress. However, higher per cent increase was observed in tolerant genotypes. Heat stress increased the SOD activity in tolerant genotypes but activity declined in susceptible genotypes. On revival, the activities of the CAT, POX and GR declined in comparison to stressed seedlings but remained higher as compared to control. Accumulation of H2O2, which is a strong oxidant, led to disruption of cellular membrane integrity as obvious from increase in MDA content and an indicator of lipid peroxidation. Higher increase of H2O2 and MDA content was observed in susceptible genotypes which indicate more oxidative stress in the heat susceptible genotypes as compared to heat tolerant genotypes. On revival, accumulation of H2O2 and MDA content was reduced in all the genotypes but their level remained higher than their respective controls. A large number of studies have revealed a positive correlation between induction of HSPs and acquisition of thermotolerance. The most studied effect of heat shock treatment was on the induction of protective heat shock proteins which disappeared on removal of heat stress. In tolerant genotype WH-1021 under stressed conditions five polypeptide bands of MW 103.7, 92.2, 83, 65 and 26 kDa appeared in comparison to unstressed seedlings, while HW-2045 showed five bands of MW 103.7, 98.5, 79, 62 and 26 kDa under heat stress. Both the susceptible genotypes showed similar polypeptide bands of MW 80, 57.9, 45.7 and 34.4 kDa in response to high temperature. The better tolerance character of HW-2045 and WH-1021 during present investigation might be due to the induction of protective heat shock proteins and much higher activities of the enzymes involved in scavenging active oxygen species.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
Heat stress increased leaf H2O2 production in both tolerant and susceptible wheat genotypes.
- Taxon
- wheat
Triticum aestivumt0 - Organ / stage
- leavese0
- Treatment
- heat stress conditionse0
- Outcome
- production of H2O2e0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The production of H2O2 was higher in the leaves of susceptible and tolerant genotypes under heat stress conditions.”
t0“wheat cultivars”
Claim · model-written summary
Under heat stress, susceptible wheat genotypes had significantly more H2O2 than tolerant genotypes.
- Taxon
- wheatTriticum aestivumt0
- Material
- susceptiblee0
- Outcome
- its levele0
- Direction
- highere0
- Comparator
- tolerant genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, its level was significantly higher in susceptible as compared to tolerant genotypes.”
t0“wheat cultivars”
Claim · model-written summary
Heat stress enhanced CAT, POX, GR and APX activity in leaves of both wheat genotype classes.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- leavese0
- Treatment
- heat stresse0
- Outcome
- activities of antioxidative enzymes, viz. CAT, POX, GR and APXe0
- Direction
- enhancemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was enhancement in the activities of antioxidative enzymes, viz. CAT, POX, GR and APX in the leaves of tolerant and susceptible genotypes under heat stress.”
t0“wheat cultivars”
Claim · model-written summary
The percentage increase in antioxidative enzyme activity under heat stress was higher in tolerant wheat genotypes.
- Taxon
- wheatTriticum aestivumt0
- Material
- tolerant genotypese0
- Outcome
- per cent increasee0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, higher per cent increase was observed in tolerant genotypes.”
t0“wheat cultivars”
Claim · model-written summary
Heat stress increased SOD activity in tolerant wheat genotypes.
- Taxon
- wheatTriticum aestivumt0
- Material
- tolerant genotypese0
- Treatment
- Heat stresse0
- Outcome
- SOD activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heat stress increased the SOD activity in tolerant genotypes but activity declined in susceptible genotypes.”
t0“wheat cultivars”
Claim · model-written summary
Heat stress decreased SOD activity in susceptible wheat genotypes.
- Taxon
- wheatTriticum aestivumt0
- Material
- susceptible genotypese0
- Treatment
- Heat stresse0
- Outcome
- SOD activitye0
- Direction
- declinede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heat stress increased the SOD activity in tolerant genotypes but activity declined in susceptible genotypes.”
t0“wheat cultivars”
Claim · model-written summary
After revival, CAT, POX and GR activities declined relative to stressed wheat seedlings but remained above controls.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- revivale0
- Outcome
- activities of the CAT, POX and GRe0
- Direction
- declinede0
- Qualifier
- remained higher as compared to controle0
- Comparator
- stressed seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On revival, the activities of the CAT, POX and GR declined in comparison to stressed seedlings but remained higher as compared to control.”
t0“wheat cultivars”
Claim · model-written summary
Higher H2O2 accumulation in wheat was linked by the authors to disrupted membranes and greater MDA content.
- Taxon
- wheatTriticum aestivumt0
- Compound
- H2O2e0
- Outcome
- disruption of cellular membrane integritye0
- Qualifier
- increase in MDA contente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of H2O2, which is a strong oxidant, led to disruption of cellular membrane integrity as obvious from increase in MDA content and an indicator of lipid peroxidation.”
t0“wheat cultivars”
Claim · model-written summary
Susceptible wheat genotypes showed a greater increase in H2O2 and MDA content than tolerant genotypes.
- Taxon
- wheatTriticum aestivumt0
- Material
- susceptible genotypese0
- Outcome
- H2O2 and MDA contente0
- Direction
- Higher increasee0
- Comparator
- heat tolerant genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher increase of H2O2 and MDA content was observed in susceptible genotypes which indicate more oxidative stress in the heat susceptible genotypes as compared to heat tolerant genotypes.”
t0“wheat cultivars”
Claim · model-written summary
After revival, H2O2 and MDA content fell in all wheat genotypes but stayed above controls.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- revivale0
- Outcome
- accumulation of H2O2 and MDA contente0
- Direction
- reducede0
- Comparator
- their respective controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On revival, accumulation of H2O2 and MDA content was reduced in all the genotypes but their level remained higher than their respective controls.”
t0“wheat cultivars”
Claim · model-written summary
Heat stressed WH-1021 seedlings displayed five new polypeptide bands.
- Taxon
- wheatTriticum aestivumt0
- Material
- WH-1021e0
- Treatment
- stressed conditionse0
- Outcome
- polypeptide bandse0
- Value
- 103.7, 92.2, 83, 65 and 26 kDae0
- Comparator
- unstressed seedlingse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In tolerant genotype WH-1021 under stressed conditions five polypeptide bands of MW 103.7, 92.2, 83, 65 and 26 kDa appeared in comparison to unstressed seedlings”
t0“wheat cultivars”
Claim · model-written summary
Heat stressed HW-2045 seedlings displayed five polypeptide bands.
- Taxon
- wheatTriticum aestivumt0
- Material
- HW-2045e0
- Treatment
- heat stresse0
- Outcome
- five bandse0
- Value
- 103.7, 98.5, 79, 62 and 26 kDae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while HW-2045 showed five bands of MW 103.7, 98.5, 79, 62 and 26 kDa under heat stress.”
t0“wheat cultivars”
Claim · model-written summary
The two susceptible wheat genotypes showed similar high temperature induced polypeptide bands.
- Taxon
- wheatTriticum aestivumt0
- Material
- susceptible genotypese0
- Treatment
- high temperaturee0
- Outcome
- similar polypeptide bandse0
- Value
- 80, 57.9, 45.7 and 34.4 kDae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the susceptible genotypes showed similar polypeptide bands of MW 80, 57.9, 45.7 and 34.4 kDa in response to high temperature.”
t0“wheat cultivars”
Claim · model-written summary
Authors suggest greater heat tolerance of HW-2045 and WH-1021 may reflect heat shock proteins and oxygen scavenging enzymes.
- Taxon
- wheatTriticum aestivumt0
- Material
- HW-2045 and WH-1021e0
- Outcome
- better tolerance charactere0
- Qualifier
- induction of protective heat shock proteins and much higher activities of the enzymes involved in scavenging active oxygen speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The better tolerance character of HW-2045 and WH-1021 during present investigation might be due to the induction of protective heat shock proteins and much higher activities of the enzymes involved in scavenging active oxygen species.”
t0“wheat cultivars”
The same questions in other papers
heat · physiology: antioxidant enzymes · Triticum aestivum (wheat)Results differ
16 papers in the same direction as this paper · 11 in the opposite direction
- negative
- negative
- negative
24 papers more in this group
See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.