Abstract
기내에서 배양되고 있는 감자 (Solanum tuberosum L.)로부터 액아와 완전히 전개된 잎이 부착된 약 1 cm 크기의 절간을 채취하였다. 절간의 잎은 제거한 후 온도, 일장, sucrose, CCC의 처리가 소괴경의 형성과 비대에 미치는 영향에 관하여 확인하기 위해 MS배지에서 배양을 하였다. 기내 소괴경의 형성에 있어 $20^{\circ}C$와 $28^{\circ}C$의 두 처리간에 있어 배양 80일 후에 형성된 괴경의 크기나 생체중 모두 $28^{\circ}C$에 비해 $20^{\circ}C$에서 유의성 있는 효과를 보였으며, 배지에 첨가되는 sucrose의 영향에 있어서도 농도에 따른 차이가 뚜렷하여 8% sucrose가 3% 보다 괴경의 비대 발육을 촉진하였다. 일장의 영향은 배지의 sucrose의 농도의 영향을 많이 받았다. $20^{\circ}C$에서는 sucrose가 8%일 때 일장에 관계없이 괴경의 형성이 양호하였지만 sucrose의 농도가 3%일 경우에는 일장과 관계없이 액아는 신초로 발달하였다. 그러나 $28^{\circ}C$의 고온에서는 8% sucrose와 8시간의 단일에서만 괴경의 비대에 효과가 있었다. CCC의 영향에 있어서 괴경의 구중은 sucrose 농도가 8%의 배지에서는 모든 일장에서 괴경의 형성이 양호하였으며 일장이 짧을 수록 효과는 더 양호하였다. 배양온도에 따른 감자의 액아부위 세포의 조직학적 변화에 있어서는 저온조건에서 괴경이 형성이 촉진되어 세포의 조직은 신장생장보다 비후생장을 함을 관찰할 수 있었다. 일장조건에 따른 변화에 있어서는 암조건에서 조직의 비후 생장이 뚜렷함을 관찰할 수 있었으며, CCC의 첨가 유무에 따른 변화에 있어서는 CCC를 첨가한 조건에서 양호한 현상을 관찰 할 수 있었다. 또한 조직의 변화에 있어서 배양한지 9일이 되었을 때 pith, cortex 및 perimedullary tissue에 있는 parenchyma 세포의 수와 크기가 증가된 것으로 확인할 수 있었다. 특히 조직내 위치별 세포분열의 가시적 비율에서는 pith부위 보다 cortex부위에서 세포비후의 비율이 큰 것으로 보였다. Sucrose, CCC 및 일장차이에 따른 AGPase 활성의 변화에 있어서 암처리에서는 sucrose 농도 3%보다 8%에서 양호하였고 sucrose 3%, 8%에서도 각각 CCC의 효과를 볼 수 있었다. 8시간 처리에서는 sucrose 농도 3%보다 8%에서 양호하였고 sucrose 3%, 8%에서는 CCC의 효과가 나타나지 않았다. 16시간 처리 sucrose 농도 3%보다는 8%에서 양호하였고 sucrose 3%에서는 CCC의 효과가 나타나지 않았으나 8%에서는 CCC의 효과를 볼 수 있었다. 결과적으로 sucrose의 농도가 높고, CCC와 암처리의 괴경형성의 촉진조건에서 AGPase의 활성이 양호함을 볼 때, AGPase가 starch 합성에 중요한 물질이라는 사실이 확인되었다. One-node stem pieces ca. 1 cm in length containing a axillary bud and a fully expanded leaf were obtained from it in vitro plants of potato (Solanum tuberosum L.). Leaves were removed and the nodes were cultured on the MS medium to investigate the effects of temperature, day length, sucrose, and CCC in microtuber formation and development. The fresh weight of microtubers after 80 days increased significantly at 8% sucrose and $20^{\circ}C$ compared with $28^{\circ}C$. The tuberization and development were reduced at $28^{\circ}C$ except short-day treatment of 8 hours at 8% sucrose. The fresh weight and diameter were increased on the culture medium added CCC 500 mg/L. The potato tuberization was promoted under short daylength, and it showed great effect by treatment with the CCC. Though the tuberization was promoted at low temperature of $20^{\circ}C$ in a histologic change of an axillary bud part cell of a potato, the cells were able to observe the swelling growth. Swelling growth of tissue was stimulated in the darkness and was more remarkable by addition of CCC. In particular, in the visual ratio of cell division for each position in the tissue, the cortex part showed larger ratio of cell expansion than that of the pith part. The effect of CCC was identified at 8% sucrose in the darkness. The effect of CCC was not showed in sucrose 3% under long daylength of 16 hours. As a result, the fact of a substance with AGPase important for starch composition was certified by the result with the inclose of AGPase activity on high concentration of sucrose, CCC, and dark treatment by which tuber formation and development are promoted.
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Claims 11
Claim · model-written summary
Microtuber fresh weight increased significantly at 8% sucrose and 20C versus 28C.
- Taxon
- potato
Solanum tuberosumt0 - Treatment
- 8% sucrose and $20^{\circ}C$e0
- Outcome
- fresh weight of microtuberse0
- Direction
- increasede0
- Comparator
- $28^{\circ}C$e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fresh weight of microtubers after 80 days increased significantly at 8% sucrose and $20^{\circ}C$ compared with $28^{\circ}C$”
t0“Potato”
Claim · model-written summary
Tuberization and development were reduced at 28C except under an eight hour day with 8% sucrose.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- $28^{\circ}C$e0
- Outcome
- tuberization and developmente0
- Direction
- reducede0
- Qualifier
- except short-day treatment of 8 hours at 8% sucrosee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tuberization and development were reduced at $28^{\circ}C$ except short-day treatment of 8 hours at 8% sucrose”
t0“Potato”
Claim · model-written summary
CCC increased microtuber fresh weight.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- CCC 500 mg/Le0
- Outcome
- fresh weighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fresh weight and diameter were increased on the culture medium added CCC 500 mg/L”
t0“Potato”
Claim · model-written summary
CCC increased microtuber diameter.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- CCC 500 mg/Le0
- Outcome
- diametere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fresh weight and diameter were increased on the culture medium added CCC 500 mg/L”
t0“Potato”
Claim · model-written summary
Short daylength promoted potato tuberization.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- short daylengthe0
- Outcome
- tuberizatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The potato tuberization was promoted under short daylength”
Claim · model-written summary
Low temperature promoted tuberization and cell swelling.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- low temperature of $20^{\circ}C$e0
- Outcome
- tuberizatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tuberization was promoted at low temperature of $20^{\circ}C$ in a histologic change of an axillary bud part cell of a potato, the cells were able to observe the swelling growth”
Claim · model-written summary
Darkness stimulated tissue swelling growth.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- darknesse0
- Outcome
- Swelling growth of tissuee0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Swelling growth of tissue was stimulated in the darkness”
t0“Potato”
Claim · model-written summary
CCC made tissue swelling more remarkable.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- addition of CCCe0
- Outcome
- Swelling growth of tissuee0
- Direction
- more remarkablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Swelling growth of tissue was stimulated in the darkness and was more remarkable by addition of CCC”
t0“Potato”
Claim · model-written summary
Cortex had a larger ratio of cell expansion than pith.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- cortex parte0
- Outcome
- ratio of cell expansione0
- Direction
- largere0
- Comparator
- pith parte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cortex part showed larger ratio of cell expansion than that of the pith part”
t0“Potato”
Claim · model-written summary
CCC had an effect at 8% sucrose in darkness.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- CCCe0
- Outcome
- effect of CCCe0
- Qualifier
- 8% sucrose in the darknesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of CCC was identified at 8% sucrose in the darkness”
t0“Potato”
Claim · model-written summary
CCC showed no effect at 3% sucrose under 16 hour long days.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- CCCe0
- Outcome
- effect of CCCe0
- Direction
- not showede0
- Qualifier
- sucrose 3% under long daylength of 16 hourse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of CCC was not showed in sucrose 3% under long daylength of 16 hours”
t0“Potato”
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See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.