Paper · 1997 · Biologia Plantarum

Nutrient-encapsulation of potato nodal segments for germplasm exchange and distribution

D. Sarkar; P.S. Naik

6
claims
10
verbatim quotes
7
citations
2
research areas
—
Not open access

Abstract

Nutrient-encapsulation technique using in vitro grown nodal segments was developed as an alternative method for distribution of potato germplasm. The nodal cuttings of two potato genotypes, Kufri Jyoti and Kufri Lauvkar, were encapsulated in calcium-free Murashige and Skoog's (MS) medium containing either 2 or 3 % sodium alginate and 0, 1, 2 or 3 % saccharose. The encapsulated segments were stored in tubes with or without semisolid MS medium, and incubated in the dark at 25 ± °C for 3 to 6 weeks. Presence of saccharose in the beads was found detrimental for regrowth of new shoots. In absence of saccharose, about 82 % and 53 % encapsulated segments initiated regrowth after 3 and 6 weeks of dark storage, respectively, in tubes containing MS medium. Storage in empty tubes deteriorated the encapsulated segments, and depressed shoot formation. For potato germplasm distribution, the encapsulated segments can be transported in small tubes containing semisolid MS medium.

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Claims 6

Method / resourceobservedoriginal resultpositivebasis n/a

Claim · model-written summary

Nutrient encapsulation of in vitro nodal segments was developed for distributing potato germplasm.

Organ / stage
nodal segmentse0
Outcome
distribution of potato germplasme0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Nutrient-encapsulation technique using in vitro grown nodal segments was developed as an alternative method for distribution of potato germplasm.”

✓ located verbatim in the source · abstract, characters 0–145 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Saccharose in beads was detrimental to new shoot regrowth.

Treatment
Presence of saccharose in the beadse0
Outcome
regrowth of new shootse0
Direction
detrimentale0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Presence of saccharose in the beads was found detrimental for regrowth of new shoots.”

✓ located verbatim in the source · abstract, characters 502–587 · show in text ↑
t0

“potato”

✓ located verbatim in the source · title, characters 26–32 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Without saccharose, about 82 % of encapsulated segments regrew after three weeks of dark storage in MS-medium tubes.

Treatment
absence of saccharosee0
Outcome
initiated regrowthe0
Value
82 %e0
Qualifier
3e0
Setting
tubes containing MS mediume0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“In absence of saccharose, about 82 % and 53 % encapsulated segments initiated regrowth after 3 and 6 weeks of dark storage, respectively, in tubes containing MS medium.”

✓ located verbatim in the source · abstract, characters 588–756 · show in text ↑
t0

“potato”

✓ located verbatim in the source · title, characters 26–32 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Without saccharose, 53 % of encapsulated segments regrew after six weeks of dark storage in MS-medium tubes.

Treatment
absence of saccharosee0
Outcome
initiated regrowthe0
Value
53 %e0
Qualifier
6 weeks of dark storagee0
Setting
tubes containing MS mediume0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“In absence of saccharose, about 82 % and 53 % encapsulated segments initiated regrowth after 3 and 6 weeks of dark storage, respectively, in tubes containing MS medium.”

✓ located verbatim in the source · abstract, characters 588–756 · show in text ↑
t0

“potato”

✓ located verbatim in the source · title, characters 26–32 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Empty-tube storage deteriorated encapsulated segments and depressed shoot formation.

Treatment
Storage in empty tubese0
Outcome
shoot formatione0
Direction
depressede0
Qualifier
deteriorated the encapsulated segmentse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Storage in empty tubes deteriorated the encapsulated segments, and depressed shoot formation.”

✓ located verbatim in the source · abstract, characters 757–850 · show in text ↑
t0

“potato”

✓ located verbatim in the source · title, characters 26–32 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Encapsulated potato segments can be transported in small tubes containing semisolid MS medium.

Outcome
transported in small tubes containing semisolid MS mediume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum tuberosum
Solanum tuberosum · TaxonScott Bauer, USDA ARS · Public domain · Wikimedia Commons ↗

Verbatim

e0

“For potato germplasm distribution, the encapsulated segments can be transported in small tubes containing semisolid MS medium.”

✓ located verbatim in the source · abstract, characters 851–977 · show in text ↑

sucrose · growth · Solanum tuberosum (potato)Positive and negative results

17 papers: 13 positive, 4 negative or no effect

3 papers in the same direction as this paper · 13 in the opposite direction

  1. positive
  2. positive
  3. positive

13 papers more in this group

See all 17 papers →

storage · growth · Solanum tuberosum (potato)Positive and negative results

18 papers: 9 positive, 9 negative or no effect

8 papers in the same direction as this paper · 9 in the opposite direction

  1. positive
  2. positive
  3. positive

14 papers more in this group

See all 18 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.