Abstract
The present study aims at developing optimal media for different cultivars of Citrus namely, 'Rangpur' lime, 'C 35' citrange, 'Troyer' citrange, 'Swingle' citrumelo and 'Alemow' macrophylla. Cultures from adult field-grown trees were established from nodal stem segments for axillary proliferation. The single node segments were surface-sterilized and cultured on Murashige and Skoog (MS medium; 1962) medium. The shoot cultures obtained were used as explants to investigate the effects of various media constituents necessary for growth. Among the media constituents, cytokinins played a crucial role in shoot proliferation and thus were a prerequisite for the shoot multiplication media in all the cultivars. In case of 'Alemow' macrophylla, a synergistic effect of auxins and cytokinins was observed. The effect of sucrose concentration was also variety-dependent and significantly affected the production of axillary shoots, with either 3% or 4% being the optimal concentration depending upon the rootstock. Moreover, the influence of gelling agents also varied with each cultivar. For 'Rangpur' lime, gelrite (0.2%) was the optimum gelling agent whereas agar-gel (0.4%) was the best for the remainder four varieties. Among the five rootstocks tested, shoot proliferation rate was highest in 'Rangpur' lime and lowest in 'Alemow' macrophylla. The results demonstrate that genotypic differences played a significant role in the induction and growth of axillary shoots. Thus, in this study we were able to identify and optimize the most effective multiplication media for each cultivar, which is a necessary step to develop an efficient micropropagation protocol.
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Claims 8
Claim · model-written summary
Cytokinins were prerequisite for shoot proliferation in all cultivars.
CitrusNot reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytokinins played a crucial role in shoot proliferation and thus were a prerequisite for the shoot multiplication media in all the cultivars”
t0“CITRUS”
Claim · model-written summary
Auxins and cytokinins had a synergistic effect in Alemow macrophylla.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In case of 'Alemow' macrophylla, a synergistic effect of auxins and cytokinins was observed”
t0“CITRUS”
Claim · model-written summary
Sucrose concentration significantly affected axillary shoot production, with 3% or 4% optimal depending on rootstock.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose concentration was also variety-dependent and significantly affected the production of axillary shoots, with either 3% or 4% being the optimal concentration depending upon the rootstock”
t0“CITRUS”
Claim · model-written summary
The influence of gelling agents varied by cultivar.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the influence of gelling agents also varied with each cultivar”
t0“CITRUS”
Claim · model-written summary
Gelrite at 0.2% was optimal for Rangpur lime.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For 'Rangpur' lime, gelrite (0.2%) was the optimum gelling agent”
t0“CITRUS”
Claim · model-written summary
Agar-gel at 0.4% was best for the other four varieties.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“agar-gel (0.4%) was the best for the remainder four varieties”
t0“CITRUS”
Claim · model-written summary
Rangpur lime had the highest shoot proliferation rate among five rootstocks.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the five rootstocks tested, shoot proliferation rate was highest in 'Rangpur' lime”
t0“CITRUS”
Claim · model-written summary
Alemow macrophylla had the lowest shoot proliferation rate among five rootstocks.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot proliferation rate was highest in 'Rangpur' lime and lowest in 'Alemow' macrophylla”
t0“CITRUS”
The same questions in other papers
sucrose · yield · CitrusResults agree
2 papers in the same direction as this paper
- positive
- positive
class · growth · CitrusPositive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.