Search claims · PlantClaims

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

PDIL2-3 overexpression lines had reduced chalkiness, the opposite of the cri-line effect.

Taxon: RiceOryza sativae3
Treatment: PDIL2-3 overexpressione2
Outcome: chalky grain phenotypee1
Direction: decreaseinferrede2

Not reportedComparator

Verbatim

e1
“exhibited a chalky grain phenotype with altered storage substance accumulation and increased grain size and weight”
✓ located verbatim in the source · abstract, characters 486–600

e2
“whereas exactly opposite results were obtained for PDIL2-3 overexpression lines”
✓ located verbatim in the source · abstract, characters 602–681

e3
“Rice grain size and chalkiness are important traits that influence grain yield and quality”
✓ located verbatim in the source · abstract, characters 0–90

Title

PDIL2-3 encoding a protein disulfide isomerase-like enzyme is essential for grain yield and appearance quality in rice (Oryza sativa L.)

Xuelian Guo; Jiayi Song; Kun Xu; et al. The Crop Journal 2025. doi:10.1016/j.cj.2025.02.011

Other claims from this paper (9)

Observation PDIL2-3 was highly expressed in rice endosperm and spikelet hulls.
Intervention effect PDIL2-3-cri lines generated by CRISPR/Cas9 had a chalky grain phenotype.
Intervention effect PDIL2-3-cri lines had altered storage substance accumulation.
Intervention effect PDIL2-3-cri lines had increased grain size and weight.
Intervention effect PDIL2-3 overexpression lines had decreased grain size and weight, the opposite of the cri-line effect.
Intervention effect PDIL2-3-cri increased rice seed length mainly by increasing cell number.
Intervention effect PDIL2-3-cri increased rice seed width mainly by increasing cell size.
Association PDIL2-3 has a negative effect on cell proliferation in rice spikelet hulls, preventing DNA duplication and cell division.
Intervention effect Expression levels of cell cycle and storage synthesis genes changed significantly in PDIL2-3-cri transgenic lines.

Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Orthologs of these laccases were proposed as targets to improve grass biofuel feedstock.

Taxon: grasse0
Gene: Orthologs of these laccasese0
Outcome: improving grass feedstock for cellulosic biofuel productione0

Verbatim

e0
“Orthologs of these laccases are promising targets for improving grass feedstock for cellulosic biofuel production”
✓ located verbatim in the source · abstract, characters 1218–1331

Title

Inactivation of LACCASE8 and LACCASE5 genes in Brachypodium distachyon leads to severe decrease in lignin content and high increase in saccharification yield without impacting plant integrity

Philippe Le Bris; Yin Wang; Clément Barbereau; et al. Biotechnology for Biofuels 2019. doi:10.1186/s13068-019-1525-5

Other claims from this paper (8)

Intervention effect Double laccase inactivation reduced mature culm lignin content by 20–30% versus wild type.
Intervention effect Double laccase inactivation increased saccharification yield by 140% versus wild type.
Intervention effect Ferulic acid release after mild alkaline hydrolysis was 2.5-fold higher in the double mutant.
Observation Interfascicular fibers were primarily affected by the double mutation.
Observation Vascular bundle integrity was not impaired by the double mutation.
Observation The double mutation had no drastic impact on plant growth.
Mechanism Two Brachypodium laccases were implicated in lignification.
Observation Interfascicular fiber and metaxylem cell lignification were partly uncoupled.

Associationobservedoriginal resultmeasured

Claim · model-written summary

Rht8 was a major contributor to plant height.

Taxon: wheatTriticum aestivumt0
Gene: Rht8 locuse0
Outcome: plant heighte0

Not reportedDirection

Verbatim

e0
“A major contributor to plant height was the Rht8 locus and the reduced height allele was associated with significant increase in grains per spike (GPS) and FE, and decrease in number of spikes per square meter and test weight”
✓ located verbatim in the source · abstract, characters 881–1106

t0
“wheat quality and yield related traits”
✓ located verbatim in the source · title, characters 34–72

Title

Genome wide genetic dissection of wheat quality and yield related traits and their relationship with grain shape and size traits in an elite × non-adapted bread wheat cross

Ajay Kumar; Eder E. Mantovani; Senay Simsek; et al. PLoS ONE 2019. doi:10.1371/journal.pone.0221826

Other claims from this paper (14)

Observationobservedoriginal resultmeasured

Claim · model-written summary

The optimised multiplex PCR gave positive results for Lr19, Lr24 and Lr28.

Taxon: wheatTriticum aestivume0
Gene: Lr19, Lr24 and Lr28e3
Outcome: positive resultse3

Verbatim

e0
“Leaf rust is a fungal disease in wheat that causes significant decrease in yield around the world”
✓ located verbatim in the source · abstract, characters 0–97

e3
“we achieved positive results when running the optimised multiplex PCR tests on Lr19, Lr24 and Lr28”
✓ located verbatim in the source · abstract, characters 1653–1751

Title

Screening of Lr genes providing resistance to leaf rust in wheath using multiplex PCR method

Aybeke, Mehmet DOAJ (DOAJ: Directory of Open Access Journals) 2019

Other claims from this paper (2)

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The optimum annealing temperature for the four Lr genes in the multiplex PCR was 61°C.

Taxon: wheatTriticum aestivume0
Outcome: optimum annealing temperaturee1
Value: 61°Ce1

Verbatim

e0
“Leaf rust is a fungal disease in wheat that causes significant decrease in yield around the world”
✓ located verbatim in the source · abstract, characters 0–97

e1
“We found that the optimum annealing temperature for all four genes was 61°C”
✓ located verbatim in the source · abstract, characters 1197–1272

Title

Screening of Lr genes providing resistance to leaf rust in wheath using multiplex PCR method

Aybeke, Mehmet DOAJ (DOAJ: Directory of Open Access Journals) 2019

Other claims from this paper (2)

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Introgression of the NPB allele did not affect plant height.

Taxon: riceOryza sativat0
Treatment: introgression of the NPB allelee0
Outcome: plant heighte0
Direction: no effecte0

Not reportedComparator

Verbatim

e0
“introgression of the NPB allele resulted in a decrease in bending stress stem area and number of spikelets per panicle and an increase in tiller number but had no effect on plant height”
✓ located verbatim in the source · abstract, characters 2199–2384

t0
“rice”
✓ located verbatim in the source · title, characters 44–48

Title

Production of novel beneficial alleles of a rice yield‐related QTL by CRISPR/Cas9

Yongtao Cui; Xingming Hu; Guohua Liang; et al. Plant Biotechnology Journal 2020. doi:10.1111/pbi.13370

Other claims from this paper (19)

Association Three QTLs were associated with stem cross-section area.
Association Nipponbare alleles at qSCSA1-1 and qSCSA6-1 increased SCSA.
Association The 93-11 allele at qSCSA3-1 increased culm strength.
Observation qSCSA3-1 mapped to a 51-kb region containing OsTB1.
Observation OsTB1 expression in 93-11 inflorescences exceeded that in NPB and NIL-93-11.
Intervention effect Introgression of the NPB allele decreased bending stress.
Intervention effect Introgression of the NPB allele decreased stem area and spikelets per panicle.
Intervention effect Introgression of the NPB allele increased tiller number.
Intervention effect The OsTB1 null mutant had lower bending stress than rescued plants.
Intervention effect The OsTB1 null mutant had smaller panicles than rescued plants.
Intervention effect The OsTB1 null mutant had increased tiller number relative to rescued plants.
Observation Four large-SCSA varieties carried a TGTG insertion.
Method / resource Editing generated 23 rice plants with nine mutations.
Intervention effect Type 1 plants had more tillers and smaller culms and panicles than wild type.
Observation Type 2 plants resembled wild type phenotypically.
Intervention effect Type 3 plants had fewer tillers and larger culms and panicles than wild type.
Intervention effect Type 3 edits increased OsTB1 expression.
Intervention effect Type 1 edits reduced OsTB1 expression.
Intervention effect Type 2 edits left OsTB1 expression unchanged.

Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Smaller carpels and fewer stem water soluble carbohydrates at anthesis partly explained decreased grain weight.

Taxon: bread wheatTriticum aestivumt0
Organ / stage: carpelse0
Outcome: grain weighte0
Qualifier: pleiotropy or tight gene linkagese0

Verbatim

e0
“decrease in grain weight, partly because of smaller carpels and fewer stem water-soluble carbohydrates at anthesis caused by pleiotropy or tight gene linkages”
✓ located verbatim in the source · abstract, characters 1810–1968

t0
“bread wheat × spelt”
✓ located verbatim in the source · title, characters 75–94

Title

Optimizing tiller production and survival for grain yield improvement in a bread wheat × spelt mapping population

Quan Xie; Sean Mayes; Debbie L. Sparkes Annals of Botany 2015. doi:10.1093/aob/mcv147

Other claims from this paper (9)

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Low parent seed nitrogen in the OsNAR2.1 overexpression line decreased OsNAR2.1 expression by 55% relative to high parent seed nitrogen under high external nitrogen.

Taxon: riceOryza sativat0
Material: overexpression linee0
Treatment: low parent seed nitrogen (LPSN)e0
Gene: OsNAR2.1e0
Outcome: OsNAR2.1 expressione0
Direction: decreasee0
Value: 55%e0
Comparator: high parent seed nitrogen (HPSN)e1

Verbatim

t0
“Plant DNA methylation is sensitive to parent seed N content and influences the growth of rice”
✓ located verbatim in the source · title, characters 0–93

e0
“the low parent seed nitrogen (LPSN) in the overexpression line could lead to a decrease in the filial seed nitrogen content, total plant nitrogen content, yield, and OsNAR2.1 expression (28, 35, 23, and 55%, respectively)”
✓ located verbatim in the source · abstract, characters 581–802

e1
“compared with high parent seed nitrogen (HPSN) in high nitrogen external supply”
✓ located verbatim in the source · abstract, characters 803–882

Title

Plant DNA methylation is sensitive to parent seed N content and influences the growth of rice

Xiaoru Fan; Xiaoru Fan; Laihua Liu; et al. BMC Plant Biology 2021. doi:10.1186/s12870-021-02953-3

Other claims from this paper (7)

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The method increased the percentage of nuclear genome reads.

Taxon: soybeanGlycine maxe1
Outcome: percentage of the nuclear genomee0
Direction: increasee0
Comparator: seeds using the IMP Boom methode0

Verbatim

e0
“DNA extracted from seeds using the IMP Boom method showed an increase in the percentage of the nuclear genome with a decrease in the relative ratio of chloroplast DNA”
✓ located verbatim in the source · abstract, characters 1390–1556

e1
“soybean seeds”
✓ located verbatim in the source · abstract, characters 675–688

Title

Development of a High-Quality/Yield Long-Read Sequencing-Adaptable DNA Extraction Method for Crop Seeds

Naohiro Shioya; Eri Ogiso-Tanaka; Masanori Watanabe; et al. Plants 2023. doi:10.3390/plants12162971

Other claims from this paper (9)

Method / resource The method yielded approximately 8 µg DNA per gram of soybean seed.
Method / resource The method gave an average soybean seed DNA concentration of 48.3 ng/µL, about 40-fold above a common kit.
Method / resource The extracted DNA had an A260/280 value of 1.90 above the quality threshold.
Method / resource The extracted DNA had an A260/230 value of 2.43 above the quality threshold.
Method / resource The DNA integrity number was 8.1, above the kit and CTAB methods.
Method / resource Long-read sequencing gave a read length N50 of 20.96 kbp, above other methods.
Method / resource Long-read sequencing gave a maximum read length of 127.8 kbp, above other methods.
Method / resource The method decreased the relative ratio of chloroplast DNA.
Method / resource The method could also be adapted to pea, okra, maize and sunflower seeds.

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Loss of tsh4 enhanced the double-mutant tassel branch phenotype.

Taxon: MaizeZea mayst0
Treatment: loss of a related gene called tasselsheath4 (tsh4)e0
Outcome: tassel branch numbere1
Direction: enhancede0

Not reportedComparator

Verbatim

t0
“Maize”
✓ located verbatim in the source · title, characters 0–5

e0
“both of which are enhanced by loss of a related gene called tasselsheath4 (tsh4)”
✓ located verbatim in the source · abstract, characters 497–577

e1
“a decrease in tassel branch number”
✓ located verbatim in the source · abstract, characters 427–461

Title

Maize SBP-box transcription factors unbranched2 and unbranched3 affect yield traits by regulating the rate of lateral primordia initiation

George S. Chuck; Patrick J. Brown; Robert Meeley; et al. Proceedings of the National Academy of Sciences 2014. doi:10.1073/pnas.1407401112

Other claims from this paper (9)

“yield decrease”

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