Paper · 1976 · OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)

Ozone pretreatment protects soybean foliage against a bacteria-induced hypersensitive response

F. L. Lukezic; Eva J. Pell; R.G. Levine

6
claims
17
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Glycine max (L) Morrill, were grown in the greenhouse until the first trifoliate leaf was 1/3 expanded and exposed to 598 ..mu..g/m/sup 3/ (0.30 ppm) ozone for 3 or 4 h at 20/sup 0/C and 70% RH. Ozone at this concentration induced a low level of injury to primary leaves. At 24, 48, 96, 144 and 168 h after ozone exposure, bacteria were infiltrated into one primary leaf of a set of two plants/pot with the use of a vacuum desiccator. The opposite leaf and leaves of companion plants served as controls. Inoculum was a water suspension of 3 x 10/sup 7/ cells/ml of a Pseudomonas sp. that induced a hypersensitive response (HR). Inoculum at this concentration induced the typical rapid tissue collapse associated with HR. Plants were placed in plastic bags for 24 h after inoculation and maintained in the greenhouse. Ozone injury and HR were evaluated on the basis of surface area and intensity of symptoms. There were no significant differences in ozone injury levels in foliage when bacteria were subsequently infiltrated. Inhibition of HR was observed when leaves were inoculated 24 and 48 h after ozone exposure in each of 4 experiments. Inhibition of HR was observedmore » when leaves were inoculated 24 and 48 h after ozone exposure in each of 4 experiments. Inhibition of HR was observed in 2 of 3 experiments 96 h after ozonation. Inhibition of HR was apparent in 1 of 2 experiments conducted 144 h after inoculation and not at all 168 h after ozone exposure.« less

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Claims 6

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Ozone exposure induced a low level of injury to soybean primary leaves.

Taxon
soybeanGlycine maxt0
Treatment
Ozone at this concentratione1
Outcome
injury to primary leavese1
Qualifier
low levele1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Ozone pretreatment protects soybean foliage against a bacteria-induced hypersensitive response”

✓ located verbatim in the source · title, characters 0–94 · show in text ↑
e1

“Ozone at this concentration induced a low level of injury to primary leaves”

✓ located verbatim in the source · abstract, characters 195–270 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Bacterial infiltration after ozone exposure did not change ozone injury levels in soybean foliage.

Taxon
soybeanGlycine maxt0
Treatment
bacteriae1
Interactor
bacteriaBacteriae1pathogen
Outcome
ozone injury levelse1
Direction
no significant differencese1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Ozone pretreatment protects soybean foliage against a bacteria-induced hypersensitive response”

✓ located verbatim in the source · title, characters 0–94 · show in text ↑
e1

“There were no significant differences in ozone injury levels in foliage when bacteria were subsequently infiltrated”

✓ located verbatim in the source · abstract, characters 908–1023 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ozone exposure inhibited the Pseudomonas-induced HR in soybean when leaves were inoculated 24 and 48 h later, in each of 4 experiments.

Taxon
soybeanGlycine maxt0
Treatment
ozone exposuree1
Interactor
Pseudomonas sp.Pseudomonase2pathogen
Outcome
HRe1
Direction
Inhibitione1
Qualifier
in each of 4 experimentse1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗
Pseudomonas
Pseudomonas · Interactor · picture of the genus (one of its species)CDC · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Ozone pretreatment protects soybean foliage against a bacteria-induced hypersensitive response”

✓ located verbatim in the source · title, characters 0–94 · show in text ↑
e1

“Inhibition of HR was observed when leaves were inoculated 24 and 48 h after ozone exposure in each of 4 experiments”

✓ located verbatim in the source · abstract, characters 1025–1140 · show in text ↑
e2

“of a Pseudomonas sp. that induced a hypersensitive response (HR)”

✓ located verbatim in the source · abstract, characters 562–626 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ozone exposure inhibited HR in soybean in 2 of 3 experiments when inoculated 96 h after ozonation.

Taxon
soybeanGlycine maxt0
Treatment
ozonatione1
Interactor
Pseudomonas sp.Pseudomonase2pathogen
Outcome
HRe1
Direction
Inhibitione1
Qualifier
2 of 3 experimentse1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗
Pseudomonas
Pseudomonas · Interactor · picture of the genus (one of its species)CDC · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Ozone pretreatment protects soybean foliage against a bacteria-induced hypersensitive response”

✓ located verbatim in the source · title, characters 0–94 · show in text ↑
e1

“Inhibition of HR was observed in 2 of 3 experiments 96 h after ozonation”

✓ located verbatim in the source · abstract, characters 1265–1337 · show in text ↑
e2

“of a Pseudomonas sp. that induced a hypersensitive response (HR)”

✓ located verbatim in the source · abstract, characters 562–626 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ozone pretreatment inhibited HR in soybean in 1 of 2 experiments inoculated 144 h after ozone exposure.

Taxon
soybeanGlycine maxt0
Treatment
ozone exposuree2
Interactor
Pseudomonas sp.Pseudomonase3pathogen
Outcome
HRe1
Direction
Inhibitione1
Qualifier
1 of 2 experimentse1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗
Pseudomonas
Pseudomonas · Interactor · picture of the genus (one of its species)CDC · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Ozone pretreatment protects soybean foliage against a bacteria-induced hypersensitive response”

✓ located verbatim in the source · title, characters 0–94 · show in text ↑
e1

“Inhibition of HR was apparent in 1 of 2 experiments conducted 144 h after inoculation”

✓ located verbatim in the source · abstract, characters 1339–1424 · show in text ↑
e2

“Inhibition of HR was apparent in 1 of 2 experiments conducted 144 h after inoculation and not at all 168 h after ozone exposure”

✓ located verbatim in the source · abstract, characters 1339–1466 · show in text ↑
e3

“of a Pseudomonas sp. that induced a hypersensitive response (HR)”

✓ located verbatim in the source · abstract, characters 562–626 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Ozone exposure did not inhibit HR in soybean when leaves were inoculated 168 h after ozone exposure.

Taxon
soybeanGlycine maxt0
Treatment
ozone exposuree1
Interactor
Pseudomonas sp.Pseudomonase2pathogen
Outcome
HRe1
Direction
not at alle1
Qualifier
168 he1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗
Pseudomonas
Pseudomonas · Interactor · picture of the genus (one of its species)CDC · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Ozone pretreatment protects soybean foliage against a bacteria-induced hypersensitive response”

✓ located verbatim in the source · title, characters 0–94 · show in text ↑
e1

“Inhibition of HR was apparent in 1 of 2 experiments conducted 144 h after inoculation and not at all 168 h after ozone exposure”

✓ located verbatim in the source · abstract, characters 1339–1466 · show in text ↑
e2

“of a Pseudomonas sp. that induced a hypersensitive response (HR)”

✓ located verbatim in the source · abstract, characters 562–626 · show in text ↑

inoculation · Bacteria · Glycine max (soybean)Positive and negative results

10 papers: 8 positive, 2 negative or no effect

1 paper in the same direction as this paper · 8 in the opposite direction

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

ozone · crop injury · Glycine max (soybean)Results agree

7 papers: 0 positive, 7 negative or no effect

6 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.