Abstract
Abstract Juice sac granulation, a globally pervasive physiological disorder in citrus (Citrus spp.), leads to substantial economic losses annually. The disorder is characterized by the marked accumulation of cell wall components, yet the molecular mechanisms coordinating this process remain poorly understood. Here, we found that high temperature acts as a strong inducer of citrus granulation, triggering a rapid and pronounced accumulation of cellulose and hemicellulose in juice sacs—an early hallmark of the disorder. Through integrated transcriptomic, biochemical, and genetic analyses, we identified Cellulose synthase A 7/8 (CitCesA7/8) and IRREGULAR XYLEM 9/9H/14H (CitIRX9/9H/14H) as essential biosynthetic genes underlying this process. We further revealed that the NAM/ATAF/CUC (NAC) transcription factor CitNAC76 directly responds to high temperature and activates CitCesA7/8 and CitIRX9/9H/14H, acting as a central regulatory hub of cellulose and hemicellulose accumulation during granulation process. Notably, we uncovered a molecular ‘brake’, CitNAC71, which suppresses CitNAC76 expression and compromises its transactivation of CitCesA7/8 and CitIRX9/9H/14H, forming a dual-layered feedback module that fine-tunes cellulose and hemicellulose biosynthesis under heat stress. Together, our study unveiled the CitNAC71-CitNAC76 regulatory circuit that orchestrates stress-induced cell wall remodeling in citrus fruit, providing potential genetic targets for mitigating juice sac granulation and improving postharvest performance in a warming climate.
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Claims 4
Claim · model-written summary
High temperature induced citrus juice-sac granulation and rapid cellulose and hemicellulose accumulation.
- Organ / stage
- juice sacse0
- Treatment
- high temperaturee0
- Outcome
- cellulose and hemicellulose in juice sacse0
- Direction
- accumulatione0
CitrusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high temperature acts as a strong inducer of citrus granulation, triggering a rapid and pronounced accumulation of cellulose and hemicellulose in juice sacs”
Claim · model-written summary
CitCesA7/8 and CitIRX9/9H/14H were identified as essential biosynthetic genes for granulation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“citrus granulation”
e1“identified Cellulose synthase A 7/8 (CitCesA7/8) and IRREGULAR XYLEM 9/9H/14H (CitIRX9/9H/14H) as essential biosynthetic genes underlying this process”
Claim · model-written summary
CitNAC76 responds to high temperature and activates CitCesA7/8 and CitIRX9/9H/14H.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“citrus granulation”
e1“CitNAC76 directly responds to high temperature and activates CitCesA7/8 and CitIRX9/9H/14H”
Claim · model-written summary
CitNAC71 suppresses CitNAC76 expression and compromises activation of the biosynthetic genes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“citrus fruit”
e1“CitNAC71, which suppresses CitNAC76 expression and compromises its transactivation of CitCesA7/8 and CitIRX9/9H/14H”
The same questions in other papers
heat · molecular: metabolites · CitrusPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.