Abstract
Potato (Solanum tuberosum L.) is the world’s fourth-largest staple crop. Alkaline salt stress is a major abiotic stress factor that severely limits the growth, yield, and quality of potatoes; however, little is known about the molecular basis of potatoes’ response to alkaline salt stress or the stress-alleviation mechanism mediated by 24-epibrassinoside. In this study, we conducted a genome-wide identification of the potato miR482 family and analyzed its response patterns under alkaline salt stress and 24-epibrassinoside-mediated stress relief. We identified a total of 9 mature stu-miR482 sequences and 5 precursor sequences; all precursors form typical stable hairpin structures and exhibit high evolutionary conservation among Solanaceae plants. Promoter analysis revealed multiple cis-acting elements in the promoter region associated with light signaling, plant hormones, and stress signaling. A total of 64 potential target genes were predicted, encompassing transcription factors, disease resistance, and signal transduction-related genes, forming a complex regulatory network. Phenotypic analysis confirmed that EBR significantly alleviates the growth inhibition in potatoes induced by alkaline salt stress. qRT-PCR analysis indicated that stu-miR482a-5p is the primary stress-responsive member in leaves; stu-miR482d-3p/5p exhibited the strongest regulatory response to EBR in roots; in potato stolons, all members of the miR482 family were significantly upregulated under alkaline salt stress, with stu-miR482d-5p showing extremely significant upregulation across all treatment groups. In summary, this study represents the first systematic characterization of the potato miR482 family, revealing its tissue differential functions in alkaline salt stress and EBR-mediated stress relief.
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Claims 9
Claim · model-written summary
The potato miR482 family contains nine mature and five precursor sequences.
- Taxon
- potato
Solanum tuberosumt0 - Gene
- stu-miR482e0
- Outcome
- mature stu-miR482 sequences and 5 precursor sequencese0
- Value
- 9 mature stu-miR482 sequences and 5 precursor sequencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified a total of 9 mature stu-miR482 sequences and 5 precursor sequences”
t0“Potato”
Claim · model-written summary
All miR482 precursors have stable hairpin structures and high conservation among Solanaceae.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- typical stable hairpin structures and exhibit high evolutionary conservatione0
- Qualifier
- among Solanaceae plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all precursors form typical stable hairpin structures and exhibit high evolutionary conservation among Solanaceae plants”
t0“Potato”
Claim · model-written summary
The promoters contain cis-acting elements associated with light, hormone, and stress signaling.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- cis-acting elements in the promoter regione0
- Qualifier
- associated with light signaling, plant hormones, and stress signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promoter analysis revealed multiple cis-acting elements in the promoter region associated with light signaling, plant hormones, and stress signaling”
t0“Potato”
Claim · model-written summary
Sixty-four potential miR482 target genes were predicted.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- potential target genese0
- Value
- 64e0
- Qualifier
- transcription factors, disease resistance, and signal transduction-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 64 potential target genes were predicted, encompassing transcription factors, disease resistance, and signal transduction-related genes”
t0“Potato”
Claim · model-written summary
EBR significantly alleviated alkaline salt induced growth inhibition in potato.
- Taxon
- potatoesSolanum tuberosume0
- Treatment
- EBRe0
- Outcome
- growth inhibitione0
- Direction
- alleviatese0
- Qualifier
- alkaline salt stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EBR significantly alleviates the growth inhibition in potatoes induced by alkaline salt stress”
Claim · model-written summary
stu-miR482a-5p is the primary stress-responsive member in leaves.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- leavese0
- Gene
- stu-miR482a-5pe0
- Outcome
- primary stress-responsive membere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stu-miR482a-5p is the primary stress-responsive member in leaves”
t0“Potato”
Claim · model-written summary
stu-miR482d-3p/5p had the strongest EBR response in roots.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- rootse0
- Gene
- stu-miR482d-3p/5pe0
- Outcome
- regulatory response to EBRe0
- Direction
- strongeste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stu-miR482d-3p/5p exhibited the strongest regulatory response to EBR in roots”
t0“Potato”
Claim · model-written summary
All miR482 members were significantly upregulated in potato stolons under alkaline salt stress.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- stolonse0
- Treatment
- alkaline salt stresse0
- Gene
- miR482 familye0
- Outcome
- members of the miR482 familye0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in potato stolons, all members of the miR482 family were significantly upregulated under alkaline salt stress”
Claim · model-written summary
stu-miR482d-5p was extremely significantly upregulated across treatment groups.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- stu-miR482d-5pe0
- Outcome
- upregulatione0
- Direction
- upregulatione0
- Qualifier
- across all treatment groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stu-miR482d-5p showing extremely significant upregulation across all treatment groups”
t0“Potato”
The same questions in other papers
24-epi-brassinolide · disease · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.