Abstract
Pseudomonas protegens, a member of the P. fluorescens complex, is a key biocontrol bacterium with well-documented potential to protect plants against diverse pathogens. Although P. protegens strains have been widely exami-ned globally, those originating from Japan have not been well described. In this study, we isolated and characterized a new P. protegens strain, GSF-73, from the rhizosphere of Allium fistulosum in Gifu, Japan, and assessed its performance against several major plant diseases. Draft genome sequencing produced a 7.13-Mbp assembly, and average nucleotide identity values of 97.81-98.26% with reference strains (Cab75, CHA0, and Pf-5) confirmed its species identity. A comparative genomic anal-ysis showed that GSF-73 possessed a larger genome than the reference strains, containing eight conserved biosynthetic gene clusters for antimicrobial compounds and an expanded set of strain-specific genes related to metabolism, regulation, mobilome functions, and secretion. GSF-73 exhibited broad-spectrum antagonistic activity in vitro against fungal, oomycete, and bacterial pathogens. In biocontrol assays, GSF-73 significantly suppressed spinach Fusarium wilt, cucumber anthracnose on detached cotyledons, tomato bacterial wilt, and cucumber downy mildew. In contrast, root and/or seed treatments enhanced Pythium root rot in spinach and anthracnose in pot-grown cucumbers, indicating pathosystem-dependent efficacy. Despite these contrasting outcomes, GSF-73 shows strong biocontrol potential and merits further study to elucidate the mechanisms underlying both beneficial and adverse effects for its optimized use as a locally adapted biocontrol agent for Japanese agriculture.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
GSF-73 had an assembled genome of 7.13 Mbp.
- Taxon
- Allium fistulosum
Allium fistulosume7 - Outcome
- assemblye0
- Value
- 7.13-Mbpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Draft genome sequencing produced a 7.13-Mbp assembly”
e7“Allium fistulosum”
Claim · model-written summary
Genome identity values confirmed GSF-73 as P. protegens.
- Taxon
- Pseudomonas protegensPseudomonas protegenst0
- Outcome
- species identitye0
- Value
- 97.81-98.26%e0
Verbatim
e0“average nucleotide identity values of 97.81-98.26% with reference strains (Cab75, CHA0, and Pf-5) confirmed its species identity”
t0“Pseudomonas protegens”
Claim · model-written summary
GSF-73 had a larger genome than reference strains.
- Taxon
- Allium fistulosumAllium fistulosume7
- Outcome
- genomee0
- Direction
- largere0
- Comparator
- reference strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GSF-73 possessed a larger genome than the reference strains”
e7“Allium fistulosum”
Claim · model-written summary
GSF-73 contained eight conserved antimicrobial biosynthetic gene clusters.
- Taxon
- Allium fistulosumAllium fistulosume7
- Outcome
- biosynthetic gene clusters for antimicrobial compoundse0
- Value
- eighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“containing eight conserved biosynthetic gene clusters for antimicrobial compounds”
e7“Allium fistulosum”
Claim · model-written summary
GSF-73 had expanded strain-specific genes related to metabolism, regulation, mobilome functions and secretion.
- Taxon
- Allium fistulosumAllium fistulosume7
- Outcome
- strain-specific genese0
- Direction
- expandede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an expanded set of strain-specific genes related to metabolism, regulation, mobilome functions, and secretion”
e7“Allium fistulosum”
Claim · model-written summary
GSF-73 showed broad antagonism against plant pathogens in vitro.
- Taxon
- Allium fistulosumAllium fistulosume7
- Outcome
- antagonistic activitye0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GSF-73 exhibited broad-spectrum antagonistic activity in vitro against fungal, oomycete, and bacterial pathogens”
e7“Allium fistulosum”
Claim · model-written summary
GSF-73 significantly suppressed spinach Fusarium wilt.
- Taxon
- spinach
Spinacia oleraceae0 - Treatment
- GSF-73e0
- Second interactor
- GSF-73e0biocontrol_agent
- Outcome
- Fusarium wilte0
- Direction
- suppressede0
FusariumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GSF-73 significantly suppressed spinach Fusarium wilt”
e1“In biocontrol assays, GSF-73 significantly suppressed”
Claim · model-written summary
GSF-73 significantly suppressed cucumber anthracnose on detached cotyledons.
- Taxon
- cucumber
Cucumis sativuse0 - Organ / stage
- detached cotyledonse0
- Treatment
- GSF-73e1
- Interactor
- GSF-73e1biocontrol_agent
- Outcome
- anthracnosee0
- Direction
- suppressede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cucumber anthracnose on detached cotyledons”
e1“In biocontrol assays, GSF-73 significantly suppressed spinach Fusarium wilt, cucumber anthracnose on detached cotyledons”
Claim · model-written summary
GSF-73 significantly suppressed tomato bacterial wilt.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- GSF-73e0
- Interactor
- GSF-73e0biocontrol_agent
- Outcome
- bacterial wilte0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In biocontrol assays, GSF-73 significantly suppressed spinach Fusarium wilt, cucumber anthracnose on detached cotyledons, tomato bacterial wilt”
Claim · model-written summary
GSF-73 significantly suppressed cucumber downy mildew.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- GSF-73e0
- Interactor
- GSF-73e0biocontrol_agent
- Outcome
- downy mildewe0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In biocontrol assays, GSF-73 significantly suppressed spinach Fusarium wilt, cucumber anthracnose on detached cotyledons, tomato bacterial wilt, and cucumber downy mildew”
Claim · model-written summary
Root and/or seed treatments enhanced Pythium root rot in spinach.
- Taxon
- spinachSpinacia oleraceae0
- Treatment
- root and/or seed treatmentse0
- Outcome
- Pythium root rote0
- Direction
- enhancede0
.jpg?width=60){kind=small}
PythiumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“root and/or seed treatments enhanced Pythium root rot in spinach”
Claim · model-written summary
Root and/or seed treatments enhanced anthracnose in pot-grown cucumbers.
- Taxon
- cucumbersCucumis sativuse0
- Treatment
- root and/or seed treatmentse0
- Outcome
- anthracnosee0
- Direction
- enhancede0
- Setting
- pot-growne0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root and/or seed treatments enhanced Pythium root rot in spinach and anthracnose in pot-grown cucumbers”
The same questions in other papers
seed treatment · disease · Cucumis sativus (cucumber)Positive and negative results
1 paper in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.