Paper · 2026 · Agronomy

Biomass Production and Nitrogen Accumulation of Summer Cover Crop Mixtures Under Two Sowing Dates in a Cool Temperate Climate

Kristo Tikk; Liina Talgre; Karli Sepp; et al.

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verbatim quotes
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citations
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Open access · cc-by

Abstract

Summer cover crops can improve soil fertility and contribute to nitrogen (N) supply in temperate cropping systems, yet the effects of mixture composition and sowing timing remain insufficiently documented. This study evaluated biomass production and N accumulation of five multispecies cover crop mixtures grown in Estonia during 2024–2025 under two sowing dates per year. Aboveground biomass, botanical composition, and carbon (C) and nitrogen concentrations were measured to assess productivity, species contributions, and residue quality. Earlier sowing was generally associated with higher biomass and N accumulation, with first-sown mixtures producing, on average, 38.7% more biomass than later-sown mixtures. Mixture performance was strongly shaped by species composition and competitive hierarchies. Total N accumulation of the cover crop mixtures ranged from 42 to 275 kg N ha−1 depending on mixture composition and sowing time, with mixtures dominated by common vetch (Vicia sativa L.) achieving the highest values. Oat (Avena sativa) dominated and contributed substantially to biomass in mixtures lacking competitive legumes, whereas sunflower (Helianthus annuus) and maize (Zea mays) performed less well under delayed sowing. Low-growing species such as Persian clover (Trifolium resupinatum) produced little biomass when grown with highly competitive species. Legumes exhibited lower C:N ratios than non-legumes, while mixture-level values remained moderate, suggesting residue quality with potential for favourable decomposition and nutrient release in summer cover crop systems under temperate conditions.

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Claims 12

Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Earlier sowing was associated with greater cover crop biomass, averaging 38.7% more.

Taxon
mixturese0
Treatment
Earlier sowinge0
Outcome
biomasse0
Direction
highere0
Value
38.7%e0
Comparator
later-sown mixturese0

Verbatim

e0

“Earlier sowing was generally associated with higher biomass and N accumulation, with first-sown mixtures producing, on average, 38.7% more biomass than later-sown mixtures.”

✓ located verbatim in the source · abstract, characters 542–714 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Earlier sowing was associated with higher nitrogen accumulation.

Taxon
mixturese0
Treatment
Earlier sowinge0
Outcome
N accumulatione0
Direction
highere0

Verbatim

e0

“Earlier sowing was generally associated with higher biomass and N accumulation, with first-sown mixtures producing, on average, 38.7% more biomass than later-sown mixtures.”

✓ located verbatim in the source · abstract, characters 542–714 · show in text ↑
Observationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Species composition and competitive hierarchies strongly shaped mixture performance.

Taxon
Mixturee0
Outcome
Mixture performancee0
Qualifier
species composition and competitive hierarchiese0

Not reportedDirection

Verbatim

e0

“Mixture performance was strongly shaped by species composition and competitive hierarchies.”

✓ located verbatim in the source · abstract, characters 715–806 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Total nitrogen accumulation ranged from 42 to 275 kg N ha−1 across composition and sowing time.

Taxon
cover crop mixturese0
Outcome
Total N accumulatione0
Value
42 to 275 kg N ha−1e0
Qualifier
depending on mixture composition and sowing timee0

Verbatim

e0

“Total N accumulation of the cover crop mixtures ranged from 42 to 275 kg N ha−1 depending on mixture composition and sowing time”

✓ located verbatim in the source · abstract, characters 807–935 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Common vetch dominated mixtures had the highest nitrogen accumulation.

Taxon
mixturese0
Material
common vetch (Vicia sativa L.)e0
Outcome
highest valuese0
Direction
higheste0

Verbatim

e0

“mixtures dominated by common vetch (Vicia sativa L.) achieving the highest values.”

✓ located verbatim in the source · abstract, characters 942–1024 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Oat dominated and substantially contributed to biomass without competitive legumes.

Taxon
Oat (Avena sativa)Avena sativae0
Outcome
biomasse0
Direction
dominatede0
Qualifier
mixtures lacking competitive legumese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Avena sativa
Avena sativa · TaxonSendelbach ( talk ) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Oat (Avena sativa) dominated and contributed substantially to biomass in mixtures lacking competitive legumes”

✓ located verbatim in the source · abstract, characters 1025–1134 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Sunflower performed less well under delayed sowing.

Taxon
sunflower (Helianthus annuus)Helianthus annuuse0
Outcome
performed less welle0
Direction
less welle0
Qualifier
delayed sowinge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Helianthus annuus
Helianthus annuus · TaxonJon Sullivan · Public domain · Wikimedia Commons ↗

Verbatim

e0

“sunflower (Helianthus annuus) and maize (Zea mays) performed less well under delayed sowing.”

✓ located verbatim in the source · abstract, characters 1144–1236 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Maize performed less well under delayed sowing.

Taxon
maize (Zea mays)Zea mayse0
Outcome
performed less welle0
Direction
less welle0
Qualifier
delayed sowinge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“sunflower (Helianthus annuus) and maize (Zea mays) performed less well under delayed sowing.”

✓ located verbatim in the source · abstract, characters 1144–1236 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Persian clover produced little biomass with highly competitive species.

Taxon
Persian clover (Trifolium resupinatum)Trifolium resupinatume0
Outcome
biomasse0
Direction
littlee0
Qualifier
when grown with highly competitive speciese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium resupinatum
Trifolium resupinatum · Taxon · picture from its Wikimedia Commons categoryMacleay Grass Man · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Persian clover (Trifolium resupinatum) produced little biomass when grown with highly competitive species.”

✓ located verbatim in the source · abstract, characters 1265–1371 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Legumes had lower carbon-to-nitrogen ratios than non-legumes.

Taxon
Legumese0
Outcome
C:N ratiose0
Direction
lowere0
Comparator
non-legumese0

Verbatim

e0

“Legumes exhibited lower C:N ratios than non-legumes”

✓ located verbatim in the source · abstract, characters 1372–1423 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Mixture carbon-to-nitrogen ratios remained moderate.

Taxon
mixturee0
Outcome
mixture-level valuese0
Direction
remained moderatee0

Verbatim

e0

“mixture-level values remained moderate, suggesting residue quality with potential for favourable decomposition and nutrient release in summer cover crop systems under temperate conditions.”

✓ located verbatim in the source · abstract, characters 1431–1619 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Moderate mixture carbon-to-nitrogen ratios suggested favorable decomposition and nutrient release potential.

Taxon
summer cover crop systemse0
Outcome
favourable decomposition and nutrient releasee0
Qualifier
residue qualitye0

Verbatim

e0

“suggesting residue quality with potential for favourable decomposition and nutrient release in summer cover crop systems under temperate conditions.”

✓ located verbatim in the source · abstract, characters 1471–1619 · show in text ↑