Paper · 2026 · Scientific Reports

Interactions of nitrogen sources and foliar biostimulants differentially regulate photosynthetic efficiency and seed yield of cluster bean (Cyamopsis tetragonoloba L.) under semi-humid and arid climates

Sajjad Rahimi-Moghaddam; Zahra Rashidi; Seyyed Jalil Davarpanah; et al.

10
claims
20
verbatim quotes
0
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

Biostimulants such as humic acid (HA), salicylic acid (SA), and ascorbic acid (ASA) have gained increasing attention for enhancing crop resilience and productivity. However, their effectiveness is strongly influenced by environmental and nutritional contexts. A field experiment was conducted in two contrasting climates of Iran—semi-humid (Khorramabad) and arid (Mashhad)—to evaluate the combined effects of nitrogen sources and foliar biostimulants on physiological, biochemical, and yield traits of cluster bean (Cyamopsis tetragonoloba L.). The experiment was conducted as a factorial arrangement within a randomized complete block design with three replications. The first factor included five nitrogen treatments: control (no N), urea (69 kg N ha−1), ammonium nitrate (115 kg N ha−1), vermicompost (8 t ha−1; equivalent to 120 kg N ha−1), and biological N fertilizer containing Azotobacter spp. (Azetobarvar). The second factor comprised four foliar spray treatments: distilled water (control), HA (0.2 g L−1), ASA (200 mg L−1), and SA (200 µM). Significant three-way interactions among location, fertilizer, and biostimulant were observed for photosynthesis rate, pigments, biochemical compositions, and yield. Urea + HA consistently produced the highest photosynthetic rate and seed yield (up to 3526 kg ha−1), whereas SA enhanced stress tolerance and protein content under arid conditions. Correlation analysis showed that seed yield was positively associated with photosynthesis rate (r = 0.35**) and biological yield (r = 0.89**). Overall, HA maximized nutrient use efficiency in favorable environments, while SA and ASA enhanced biochemical performance. These findings underscore the potential of integrated nitrogen–biostimulant management for sustainable legume production across diverse climates.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 10

Associationobservedoriginal resultmixedsignificant (as stated)measured

Claim · model-written summary

Location, fertilizer, and biostimulant had a significant three-way interaction for photosynthesis rate, pigments, biochemical composition, and yield.

Taxon
cluster bean (Cyamopsis tetragonoloba L.)Cyamopsis tetragonolobat0
Treatment
fertilizer, and biostimulante0
Outcome
photosynthesis rate, pigments, biochemical compositions, and yielde0
Setting
locatione0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyamopsis tetragonoloba
Cyamopsis tetragonoloba · Taxon · picture from its Wikimedia Commons categoryTon Rulkens · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Significant three-way interactions among location, fertilizer, and biostimulant were observed for photosynthesis rate, pigments, biochemical compositions, and yield.”

✓ located verbatim in the source · abstract, characters 1052–1217 · show in text ↑
t0

“cluster bean (Cyamopsis tetragonoloba L.)”

✓ located verbatim in the source · title, characters 126–167 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Urea plus humic acid produced the highest photosynthetic rate in cluster bean.

Taxon
cluster bean (Cyamopsis tetragonoloba L.)Cyamopsis tetragonolobat0
Treatment
Urea + HAe0
Outcome
photosynthetic ratee0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyamopsis tetragonoloba
Cyamopsis tetragonoloba · Taxon · picture from its Wikimedia Commons categoryTon Rulkens · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Urea + HA consistently produced the highest photosynthetic rate and seed yield (up to 3526 kg ha−1)”

✓ located verbatim in the source · abstract, characters 1218–1317 · show in text ↑
t0

“cluster bean (Cyamopsis tetragonoloba L.)”

✓ located verbatim in the source · title, characters 126–167 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Urea plus humic acid produced the highest cluster bean seed yield, up to 3526 kg ha−1.

Taxon
cluster bean (Cyamopsis tetragonoloba L.)Cyamopsis tetragonolobat0
Treatment
Urea + HAe0
Outcome
seed yielde0
Direction
higheste0
Value
3526 kg ha−1e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyamopsis tetragonoloba
Cyamopsis tetragonoloba · Taxon · picture from its Wikimedia Commons categoryTon Rulkens · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“Urea + HA consistently produced the highest photosynthetic rate and seed yield (up to 3526 kg ha−1)”

✓ located verbatim in the source · abstract, characters 1218–1317 · show in text ↑
t0

“cluster bean (Cyamopsis tetragonoloba L.)”

✓ located verbatim in the source · title, characters 126–167 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Salicylic acid enhanced cluster bean stress tolerance under arid conditions.

Taxon
cluster bean (Cyamopsis tetragonoloba L.)Cyamopsis tetragonolobat0
Treatment
SAe0
Outcome
stress tolerancee0
Direction
enhancede0
Setting
arid conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyamopsis tetragonoloba
Cyamopsis tetragonoloba · Taxon · picture from its Wikimedia Commons categoryTon Rulkens · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“SA enhanced stress tolerance and protein content under arid conditions.”

✓ located verbatim in the source · abstract, characters 1327–1398 · show in text ↑
t0

“cluster bean (Cyamopsis tetragonoloba L.)”

✓ located verbatim in the source · title, characters 126–167 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Salicylic acid enhanced cluster bean protein content under arid conditions.

Taxon
cluster bean (Cyamopsis tetragonoloba L.)Cyamopsis tetragonolobat0
Treatment
SAe0
Outcome
protein contente0
Direction
enhancede0
Setting
arid conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyamopsis tetragonoloba
Cyamopsis tetragonoloba · Taxon · picture from its Wikimedia Commons categoryTon Rulkens · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“SA enhanced stress tolerance and protein content under arid conditions.”

✓ located verbatim in the source · abstract, characters 1327–1398 · show in text ↑
t0

“cluster bean (Cyamopsis tetragonoloba L.)”

✓ located verbatim in the source · title, characters 126–167 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Cluster bean seed yield was positively associated with photosynthesis rate (r = 0.35**).

Taxon
cluster bean (Cyamopsis tetragonoloba L.)Cyamopsis tetragonolobat0
Outcome
seed yielde0
Direction
positively associatede0
Value
r = 0.35**e0
Qualifier
photosynthesis ratee0

Not reportedCompound

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyamopsis tetragonoloba
Cyamopsis tetragonoloba · Taxon · picture from its Wikimedia Commons categoryTon Rulkens · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“seed yield was positively associated with photosynthesis rate (r = 0.35**)”

✓ located verbatim in the source · abstract, characters 1432–1506 · show in text ↑
t0

“cluster bean (Cyamopsis tetragonoloba L.)”

✓ located verbatim in the source · title, characters 126–167 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Cluster bean seed yield was positively associated with biological yield (r = 0.89**).

Taxon
cluster bean (Cyamopsis tetragonoloba L.)Cyamopsis tetragonolobat0
Outcome
seed yielde0
Direction
positively associatede0
Value
r = 0.89**e0
Qualifier
biological yielde0

Not reportedSetting

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyamopsis tetragonoloba
Cyamopsis tetragonoloba · Taxon · picture from its Wikimedia Commons categoryTon Rulkens · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“seed yield was positively associated with photosynthesis rate (r = 0.35**) and biological yield (r = 0.89**)”

✓ located verbatim in the source · abstract, characters 1432–1540 · show in text ↑
t0

“cluster bean (Cyamopsis tetragonoloba L.)”

✓ located verbatim in the source · title, characters 126–167 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Humic acid maximized nutrient use efficiency in favorable environments.

Taxon
cluster bean (Cyamopsis tetragonoloba L.)Cyamopsis tetragonolobat0
Treatment
HAe0
Outcome
nutrient use efficiencye0
Direction
maximizede0
Setting
favorable environmentse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyamopsis tetragonoloba
Cyamopsis tetragonoloba · Taxon · picture from its Wikimedia Commons categoryTon Rulkens · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“HA maximized nutrient use efficiency in favorable environments”

✓ located verbatim in the source · abstract, characters 1551–1613 · show in text ↑
t0

“cluster bean (Cyamopsis tetragonoloba L.)”

✓ located verbatim in the source · title, characters 126–167 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Salicylic acid enhanced biochemical performance in cluster bean.

Taxon
cluster bean (Cyamopsis tetragonoloba L.)Cyamopsis tetragonolobat0
Treatment
SAe0
Outcome
biochemical performancee0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyamopsis tetragonoloba
Cyamopsis tetragonoloba · Taxon · picture from its Wikimedia Commons categoryTon Rulkens · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“SA and ASA enhanced biochemical performance.”

✓ located verbatim in the source · abstract, characters 1621–1665 · show in text ↑
t0

“cluster bean (Cyamopsis tetragonoloba L.)”

✓ located verbatim in the source · title, characters 126–167 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Ascorbic acid enhanced biochemical performance in cluster bean.

Taxon
cluster bean (Cyamopsis tetragonoloba L.)Cyamopsis tetragonolobat0
Treatment
ASAe0
Outcome
biochemical performancee0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyamopsis tetragonoloba
Cyamopsis tetragonoloba · Taxon · picture from its Wikimedia Commons categoryTon Rulkens · CC BY-SA 2.0 · Wikimedia Commons ↗

Verbatim

e0

“SA and ASA enhanced biochemical performance.”

✓ located verbatim in the source · abstract, characters 1621–1665 · show in text ↑
t0

“cluster bean (Cyamopsis tetragonoloba L.)”

✓ located verbatim in the source · title, characters 126–167 · show in text ↑

salicylic acid · quality: protein · Cyamopsis tetragonoloba (cluster bean)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

nitrogen · yield · Cyamopsis tetragonoloba (cluster bean)Positive and negative results

29 papers: 28 positive, 1 negative or no effect

27 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

25 papers more in this group

See all 29 papers →

other · physiology: photosynthesis · Cyamopsis tetragonoloba (cluster bean)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

nitrogen · physiology: photosynthesis · Cyamopsis tetragonoloba (cluster bean)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

other · yield · Cyamopsis tetragonoloba (cluster bean)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.