Abstract
The rice asura mutant, which showed an increased number of stigmas, was found to contain a T-DNA insertion in one of the two alleles of the Os01g0833500 gene, encoding a serine carboxy peptidase II-like protein, SCP5. In the mutant allele, T-DNA was inserted into the region between the chain A and B domains, resulting in the generation of an aberrant transcript with a stop codon downstream of the chain A domain region. In the asura mutant, the transcripts corresponding to the chain A domain were more abundant than those corresponding to the chain B domain. From the mutant gene, an additional protein consisting only of the chain A domain was expected to be produced. This was predicted to recognize the substrates but did not exhibit catalytic activity. The phenotype of increased stigma numbers was detected in both the transformants overexpressing the chain A domain region in SCP5 and in genome-edited mutants in which the downstream region of the chain A domain was disrupted. SCP5 interacted with several regulatory proteins involved in flower morphogenesis. Furthermore, a protein consisting only of the chain A domain interacted with these proteins. These findings imply that excess production of the chain A domain protein in the asura mutant would impair the function of these regulatory proteins, resulting in insufficient control of stigma number and an increase in stigmas within spikelets.
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Claims 10
Claim · model-written summary
The rice asura mutant had increased stigma numbers and a T-DNA insertion in one Os01g0833500 allele.
- Taxon
- rice
Oryza sativae0 - Material
- asura mutante0
- Gene
- Os01g0833500e0
- Outcome
- increased number of stigmase0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rice asura mutant, which showed an increased number of stigmas, was found to contain a T-DNA insertion in one of the two alleles of the Os01g0833500 gene”
Claim · model-written summary
Insertion between the chain A and B domains generated an aberrant transcript with a downstream stop codon.
- Taxon
- riceOryza sativat0
- Gene
- T-DNAe0
- Outcome
- aberrant transcript with a stop codon downstream of the chain A domain regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“T-DNA was inserted into the region between the chain A and B domains, resulting in the generation of an aberrant transcript with a stop codon downstream of the chain A domain region”
t0“rice”
Claim · model-written summary
Chain A transcripts were more abundant than chain B transcripts in the asura mutant.
- Taxon
- riceOryza sativat0
- Material
- asura mutante0
- Outcome
- transcripts corresponding to the chain A domaine0
- Direction
- more abundante0
- Comparator
- those corresponding to the chain B domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the asura mutant, the transcripts corresponding to the chain A domain were more abundant than those corresponding to the chain B domain”
t0“rice”
Claim · model-written summary
The mutant gene was expected to produce an additional chain A only protein.
- Taxon
- riceOryza sativat0
- Outcome
- additional protein consisting only of the chain A domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From the mutant gene, an additional protein consisting only of the chain A domain was expected to be produced”
t0“rice”
Claim · model-written summary
The predicted chain A only protein would recognize substrates without catalytic activity.
- Taxon
- riceOryza sativat0
- Gene
- protein consisting only of the chain A domaine1
- Outcome
- recognize the substrates but did not exhibit catalytic activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This was predicted to recognize the substrates but did not exhibit catalytic activity”
t0“rice”
e1“additional protein consisting only of the chain A domain”
Claim · model-written summary
Transformants overexpressing the chain A domain had increased stigma numbers.
- Taxon
- riceOryza sativat0
- Treatment
- overexpressing the chain A domain region in SCP5e0
- Gene
- SCP5e0
- Outcome
- stigma numberse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phenotype of increased stigma numbers was detected in both the transformants overexpressing the chain A domain region in SCP5”
t0“rice”
Claim · model-written summary
Genome-edited mutants with disruption downstream of the chain A domain had increased stigma numbers.
- Taxon
- riceOryza sativat0
- Treatment
- downstream region of the chain A domain was disruptede0
- Outcome
- stigma numberse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in genome-edited mutants in which the downstream region of the chain A domain was disrupted”
e1“The phenotype of increased stigma numbers was detected”
t0“rice”
Claim · model-written summary
SCP5 interacted with regulatory proteins involved in flower morphogenesis.
- Taxon
- riceOryza sativat0
- Gene
- SCP5e0
- Outcome
- interacted with several regulatory proteins involved in flower morphogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SCP5 interacted with several regulatory proteins involved in flower morphogenesis”
t0“rice”
Claim · model-written summary
The chain A only protein also interacted with the flower morphogenesis regulatory proteins.
- Taxon
- riceOryza sativat0
- Gene
- protein consisting only of the chain A domaine0
- Outcome
- interacted with these proteinse0
- Qualifier
- regulatory proteins involved in flower morphogenesise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a protein consisting only of the chain A domain interacted with these proteins”
e1“several regulatory proteins involved in flower morphogenesis”
t0“rice”
Claim · model-written summary
The authors infer that excess chain A protein impairs regulatory proteins and leads to excess stigmas.
- Taxon
- riceOryza sativat0
- Material
- asura mutante0
- Gene
- chain A domain proteine0
- Outcome
- an increase in stigmas within spikeletse0
- Qualifier
- impair the function of these regulatory proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“excess production of the chain A domain protein in the asura mutant would impair the function of these regulatory proteins, resulting in insufficient control of stigma number and an increase in stigmas within spikelets”
t0“rice”